2009 Fiscal Year Final Research Report
Analysis of a novel mechanism for the p53 response to radiation by the MDM2-MDMX E3 ubiquitin ligase complex
| Project/Area Number |
20710044
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Hiroshima University |
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Principal Investigator |
KAWAI Hidehiko Hiroshima University, 原爆放射線医科学研究所, 助教 (30379846)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 放射線 / がん / P53 / MDM2 / ユビキチン化修飾 |
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| Research Abstract |
To investigate the molecular mechanisms of p53 ubiquitination, a novel in vitro assay system has been established. Using this assay system, we have found out more about how P53 is ubiquitinated by MDM2 and MDMX. Furthermore, alteration of the amount of cellular MDMX significantly affects on the response of p53 to genotoxic stress. Taken together, these results indicate that MDMX is indispensable for the regulation of the P53 response to genotoxic stress.
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