2010 Fiscal Year Final Research Report
Study on telomeric instability caused by clustered DNA damage
| Project/Area Number |
20710045
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
URUSHIBARA Ayumi Japan Atomic Energy Agency, 先端基礎研究センター, 研究員 (80391275)
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| Project Period (FY) |
2008 – 2010
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| Keywords | クラスター損傷 / テロメア / 遺伝的不安定性 / 放射線 / 紫外線 / 酸化損傷 |
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| Research Abstract |
Ionizing radiation induces genomic instability in the progeny of irradiated cells. The evidence has been accumulated to suggest that the induction of DNA double-strand breaks (DSBs) and subsequent repair processes play a key role in inducing genomic instability. In the present study, we hypothesize that some DNA lesions remained after repair of DSBs might be related to the induction of genomic instability. To study a role of clustered damage in the induction of chromosomal instability, we transferred an UVA-irradiated human chromosome 21 into unirradiated mouse m5S cells derived from mouse fibroblasts, using a microcell-mediated chromosome transfer.
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