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2009 Fiscal Year Final Research Report

Development of a molecule-dependent protein expression system that is different from natural riboswitches and its application to biosensors

Research Project

  • PDF
Project/Area Number 20750145
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionEhime University

Principal Investigator

OGAWA Atsushi  Ehime University, 上級研究員センター, 上級研究員 (30442940)

Project Period (FY) 2008 – 2009
Keywords生体機能関連化学
Research Abstract

Two novel gene regulation systems that are controlled by specific molecules have been constructed. One works in a prokaryotic translation system and the other does in a eukaryotic one. These systems are biological ones and target molecules can be changed with simple adjustments, so that they are expected to be used as versatile biosensors both in vitro and in vivo.

  • Research Products

    (6 results)

All 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results)

  • [Journal Article] Biofunction-Assisted Sensors Based on a New Method for Converting Aptazyme Activity into Reporter Protein Expression with High Efficiency in Wheat Germ Extract2009

    • Author(s)
      Atsushi Ogawa
    • Journal Title

      ChemBioChem 10

      Pages: 2465-2468

    • Peer Reviewed
  • [Journal Article] A Novel Label-Free Biosensor Using an Aptazyme-Suppressor-tRNA Conjugate and an Amber Mutated Reporter Gene2008

    • Author(s)
      Atsushi Ogawa、Mizuo Maeda
    • Journal Title

      ChemBioChem 9

      Pages: 2204-2208

    • Peer Reviewed
  • [Presentation] 真核生物の無細胞翻訳システムを利用したアプタザイム基盤バイオセンサー2010

    • Author(s)
      小川敦司
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      東大阪
    • Year and Date
      2010-03-28
  • [Presentation] Aptazyme-Based Biosensors Using a Eukaryotic Cell-Free Translation System2009

    • Author(s)
      Atsushi Ogawa
    • Organizer
      The 6th International symposium on Nucleic Acid Chemistry
    • Place of Presentation
      高山
    • Year and Date
      2009-09-29
  • [Presentation] 分子応答性タンパク質発現システムの開発2009

    • Author(s)
      小川敦司
    • Organizer
      第3回無細胞生命科学研究会
    • Place of Presentation
      弘前
    • Year and Date
      2009-03-17
  • [Presentation] サプレッサーtRNA基盤リボスイッチシステムの開発2008

    • Author(s)
      小川敦司
    • Organizer
      第3回バイオ関連化学合同シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2008-09-18

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Published: 2011-06-18   Modified: 2016-04-21  

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