2009 Fiscal Year Final Research Report
Analysis of gene silencing mechanism by bacterial small RNA
| Project/Area Number |
20770137
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
MORITA Teppei Nagoya University, 薬学部・薬学科, 助手 (10444366)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 小分子RNA / mRNA抑制 / 転写後制御 / 大腸菌 |
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| Research Abstract |
SgrS sRNA acts by baseparing with ptsG mRNA, resulting in translation inhibition and degradation of ptsG mRNA. We showed that the base pairing between SgrS and ptsG mRNA is directly responsible for translational silencing mediated by SgrS. We also identified a minimal base-pairing region of SgrS required for translational repression of ptsG mRNA. These results contribute to understanding the molecular mechanism by which Hfq-dependent sRNA act in bacteria.
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