2009 Fiscal Year Final Research Report
Determination of paternally methylated imprinted regions in mouse genome
| Project/Area Number |
20780201
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Saga University |
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Principal Investigator |
KAWAHARA Manabu Saga University, 農学部, 准教授 (70468700)
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| Project Period (FY) |
2008 – 2009
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| Keywords | ゲノムインプリンティング / 核移植 / バイオインフォマティクス / 胎盤形成 |
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| Research Abstract |
Parental genome functions in ontogeny are determined by interactions among transcripts from the maternal and paternal genomes, which contain many genes whose expression is strictly dependent on their parental origin as a result of genomic imprinting. Comprehensive recognition of the interactions between parental genomes is important for understanding genomic imprinting in mammalian development. The bi-maternal embryos were produced by the construction of oocytes from fully grown oocytes and nongrowing oocytes that contain double deletions in the H19 differentially methylated region (DMR) and the Dlk1-Dio3 intergenic germline-derived DMR. Through a sequence of studies, we demonstrated that analyses of ng/fg bi-maternal conceptuses helped in elucidating the roles of paternally methylated imprinted genes in mouse ontogeny.
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Research Products
(10 results)
