2009 Fiscal Year Final Research Report

Morphological and biochemical characterization of the Ebola virus NP-RNA complexes

Research Project

Project/Area Number	20780217
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Applied veterinary science
Research Institution	The University of Tokyo
Principal Investigator	NODA Takeshi The University of Tokyo, 医科学研究所, 特任助教 (00422410)
Project Period (FY)	2008 – 2009
Keywords	人獣共通感染症
Research Abstract	When Ebola virus nucleoprotein (NP) is expressed in mammalian cells, it assembles into helical structures. Here, the recombinant NP helix purified from cells expressing NP was characterized biochemically and morphologically. We found that the recombinant NP helix is associated with non-viral RNA which is not protected from RNase digestion and that the morphology of the helix changes depending on the environmental salt concentration. The N-terminal 450 amino acid residues of NP are sufficient for these properties. However, digestion of the NP-associated RNA eliminates the plasticity of the helix, suggesting that this RNA is an essential structural component of the helix, binding to individual NP molecules via the N-terminal 450 amino acids. These findings enhance our knowledge of Ebola virus assembly and understanding of the Ebola virus life cycle.

(1 results)