2009 Fiscal Year Final Research Report
Molecular basis for the inhibition of hypoxia-induced apoptosis by thymidine phosphorylase
Project/Area Number |
20790139
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Medical pharmacy
|
Research Institution | Kagoshima University |
Principal Investigator |
IKEDA Ryuji Kagoshima University, 医学部・歯学部附属病院, 薬剤師 (50398278)
|
Project Period (FY) |
2008 – 2009
|
Keywords | Thymidine Phosphorylase / 2-Deoxy-D-ribose / Hypoxia-inducible factor -1 |
Research Abstract |
An angiogenic factor, platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP), stimulates the chemotaxis of endothelial cells and confers resistance to apoptosis induced by hypoxia. TP and 2-deoxy-D-ribode, a degradation product of thymidine generated by TP enzymatic activity, inhibited the upregulation of hypoxia-inducible factor (HIF) 1α induced by hypoxia. We investigated the molecular basis for the suppressive effect of TP and 2-deoxy-D-ribose on the upregulation of HIF-1α. 2-Deoxy-D-ribose enhanced interaction of HIF-1α and pVHL under hypoxic condition. 2-Deoxy-D-ribose did not affect the expression of HIF-1α, prolyl hydroxylase (PHD)1/2/3 and VHL mRNA. 2-Deoxy-D-ribose also enhanced interaction of HIF-1α and PHD2 under hypoxic condition. Further study is needed to elucidate the mechanism about the augmented interaction of HIF-1α with pVHL and PHD2.
|
Research Products
(28 results)
-
[Journal Article] SUVmax of FDG-PET correlates with the effects of neoadjuvant chemoradiotherapy for oral squamous cell carcinoma2010
Author(s)
Miyawaki, A., Ikeda, R., Hijioka, H., Ishida, T., Ushiyama, M., Nozoe, E., Nakamura, N.
-
Journal Title
Oncol. Rep. 23
Pages: 1205-1212
-
[Journal Article] The role of thymidine phosphorylase in the induction of early growth response protein-1 and thrombospondin-1 by 5-fluorouracil in human cancer carcinoma cells2010
Author(s)
Matsushita, S., Ikeda, R., Nishizawa Y., Che X.F., Furukawa T., Miyadera K., Tabata S., Ushiyama M., Tajitsu Y., Yamamoto M., Takeda Y., Minami K., Mataki H., Kanzaki, T., Yamada K., Kanekura T., Akiyama S.
-
Journal Title
Int. J. Oncol. 36
Pages: 1193-1200
-
-
[Journal Article] Adverse effects of superselective intra-arterial infusion chemotherapy in patients with oral cancer2009
Author(s)
Ushiyama, M., Ikeda, R., Yamaguchi, H., Miyawaki, A., Nitta, T., Yamaguchi, T., Matsumoto, K., Shimodouzono, Y., Furukawa, T., Akiyama, S., Nakamura N., Yamada, K.
-
Journal Title
Journal of Applied Therapeutic Research 7
Pages: 58-64
-
[Journal Article] Stability of hospital preparations of Azunol Water Gargles for pain relief in oral cancer patients with oral mucositis2009
Author(s)
Ushiyama, M., Ikeda, R., Nitta, T., Tazitsu, Y., Miyawaki, A., Nishizawa, Y., Yamaguchi, T., Yamaguchi, H., Akatsuka, C., Shimodouzono, Y., Ushinohama, K., Sugawara, H., Sugihara, K., Nakamura, N., Takeda, Y., Yamada, K.
-
Journal Title
Cancer Therapy 7
Pages: 277-281
-
[Journal Article] Enhanced platelet responsiveness due to chilling and its relation to CD40 ligand level and platelet-leukocyte aggregate formation2009
Author(s)
Ayukawa, O., Nakamura, K., Kariyazono, H., Ikeda, R., Arima, J., Shinkawa, T., Iwase, H., Sakata, R., Yamada, K.
-
Journal Title
Blood Coagul. Fibrinolysis 20
Pages: 176-184
-
[Journal Article] Contactin-associated protein (Caspr) 2 interacts with carboxypeptidase E in the CNS2009
Author(s)
Oiso, S., Takeda, Y., Futagawa, T., Miura, T., Kuchiiwa, S., Nishida, K., Ikeda, R., Kariyazono, H., Watanabe, K., Yamada, K.
-
Journal Title
J. Neurochem. 109
Pages: 158-167
-
-
[Journal Article] Down regulation of c-Myc and induction of an angiogenesis inhibitor, thrombospondin-1, by 5-FU in human colon cancer KM12C cells2008
Author(s)
Zhao, H.Y., Ooyama, A., Yamamoto, M., Ikeda, R., Haraguchi, M., Tabata, S., Furukawa, T., Che, X.F., Iwashita, K., Oka, T., Fukushima, M., Nakagawa, M., Ono, M., Kuwano, M., Akiyama, S.
-
Journal Title
Cancer Lett.
Pages: 156-163
-
[Journal Article] Molecular basis for the induction of an angiogenesis inhibitor, thrombospondin-1, by 5-fluorouracil2008
Author(s)
Zhao, H.Y., Ooyama, A., Yamamoto, M., Ikeda, R., Haraguchi, M., Tabata, S., Furukawa, T., Che, X.F., Zhang, S., Oka, T., Fukushima, M., Nakagawa, M., Ono, M., Kuwano, M., Akiyama, S.
-
Journal Title
Cancer Res. 68
Pages: 7035-7041
-
[Journal Article] Hyperosmotic stress up-regulates the expression of major vault protein in SW620 human colon cancer cells2008
Author(s)
Ikeda, R., Iwashita, K., Sumizawa, T., Beppu, S., Tabata, S., Tajitsu, Y., Shimamoto, Y., Yoshida, K., Furukawa, T., Che, X.F., Yamaguchi, T., Ushiyama, M., Miyawaki, A., Takeda, Y., Yamamoto, M., Zhao, H.Y., Shibayama, Y., Yamada, K., Akiyama, S.
-
Journal Title
Exp. Cell Res. 314
Pages: 3017-3026
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-