2009 Fiscal Year Final Research Report
Analysis of repopulating dynamics of human hematopoietic stem cells
| Project/Area Number |
20790238
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Tokai University |
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Principal Investigator |
YAHATA Takashi Tokai University, 医学部, 講師 (10398753)
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| Research Collaborator |
NAKAYAMA Yukari (六車 ゆかり) 東海大学, 医学部, 研究員 (80398750)
ANDO Kiyoshi 東海大学, 医学部, 教授 (70176014)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 造血幹細胞 / ニッチ / クローン解析 / ヒト化モデルマウス / 間葉系幹細胞 |
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| Research Abstract |
Hematopoiesis is a dynamic and strictly regulated process orchestrated by self-renewing hematopoietic stem cells (HSCs) and the supporting microenvironment. However, the exact mechanisms in which individual human HSCs involved to sustain hematopoietic homeostasis remain to be clarified. To understand how the long-term repopulating cell (LTRC) activity of individual human HSCs and the hematopoietic hierarchy are maintained in the BM microenvironment, we traced the repopulating dynamics of individual human HSC clones using viral integration site analysis. Our study provides a novel insight into repopulation dynamics, turnover, hierarchical structure and the cell cycle status of human HSCs in the recipient BM microenvironment.
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