The novel mechanism of the phosphate transporter Npt2a dependent regulation of vitamin D homeostasis in kidney

2009 Fiscal Year Final Research Report

• Project/Area Number: 20790594
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokushima
• Principal Investigator: YAMAMOTO Hironori The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (60314861)
• Project Period (FY): 2008 – 2009
• Keywords: 水 / 電解質代謝学

Research Abstract

In this study, we analyzed the molecular mechanism of new regulation of vitamin D metabolism by thyroid hormone 13 in kidney. We demonstrated that thyroid hormone receptor betal and VDIR is important factor for the T3-dependent repression of the vitamin D synthesis enzyme Cyp27b1 gene. And, we found that the neither VDR nor Klotho was essential to the Cyp27bl gene control by T3, furthermore, we demonstrated that the phosphate transporter Npt2a gene play a critical role for the T3-dependent renal Cyp27bl gene repression using the Npt2a deficient mice.

Research Products

• [Journal Article] Thyroid hormones regulate phosphate homoeostasis through transcriptional control of the renal type IIa sodium-dependent phosphate co-transporter (Npt2a) gone (2010)
  • Author(s): Ishiguro M, Yamamoto H, Masuda Kozai M, Takei Y, Tanaka S, Sato T, Segawa H, Taketani Y, Arai H, Miyamoto K, Takeda E
  • Journal Title: Biochem J 427(1) Pages: 161-9
  • Peer Reviewed
• [Journal Article] Dietary phosphorus acutely impairs endothelial function (2009)
  • Author(s): Shuto F, Taketani Y, Tanaka R, Harada N, Isshiki M, Sate M, Nashiki K, Amo K, Yamamoto H, Higashi Y, Nakaya Y, Takeda E
  • Journal Title: J Am Soc Nephrol 20(7) Pages: 1504-12
  • Peer Reviewed
• [Journal Article] Receptor activator of nuclear factor-kappaB ligand-induced mouse osteoclast differentiation is associated with switching between NADPH oxidase homologues (2009)
  • Author(s): Sasaki H, Yamamoto H, Tominaga K, Masuda K, Kawai T, Teshima-Kondo S, Matsuno K, Yabe-Nishimura C, Rokutan K
  • Journal Title: Free Radic Biol Med 47(2) Pages: 189-99
  • Peer Reviewed
• [Journal Article] Hypocaloric high-protein diet improves fatty liver and hypertriglyceridemia in sucrose-fed obese rats via two pathways (2009)
  • Author(s): Cebanso T, Taketani Y, Fukaya M Sato K, Takei Y, Sato T, Sawada N, Ama K, Harada N, Arai IL Yamamoto H, Takeda E
  • Journal Title: Am J Physiol Endocrinol Metab 297(1) Pages: E76-84
  • Peer Reviewed
• [Journal Article] Dietary quercetin inhibits bone loss without effect on the uterus in ovariectomized mice (2009)
  • Author(s): Tsuji M, Yamamoto H, Sato T, Mizuha Y, Kawai Y, Taketani Y, Kato S, Terao J, Inakuma T, Takeda E
  • Journal Title: J Bone Miner Metab 27(6) Pages: 673-81
  • Peer Reviewed
• [Presentation] 食餌性リン制限および活性型ビタミンD3によるFibrobrast growth factor 15遺伝子の発現調節 (2009)
  • Author(s): 中橋乙起、山本浩範、田中更沙、菊地浩子、金子一郎、竹谷豊、宮本賢一、加藤茂明、武田英二
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] レチノイン酸受容体アイソフォーム(RARs)によるナトリウム依存性リントランスポーター遺伝子の異なる転写調節を介した小腸と腎臓でのリン恒常性維持機構 (2009)
  • Author(s): 増田真志、山本浩範、田中更沙、香西美奈、竹井悠一郎、竹谷豊、宮本賢一、武田英二
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Dual Role of Type III Sodium-Dependent Phosphate Transporter in the Pathogenesis of Arteriosclerosis Caused by Hyperphosphatemia (2009)
  • Author(s): Taketani Y, Shuto E, Shiota A, Tanimura A, Watari E, Kitamura T, Yamamoto H, Takeda E
  • Organizer: 31th Annual Meeting of the Ametican Society for Bone and Mineral Research (ASBMR)
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 20090911-20090915
• [Presentation] All-trans retinoic acid regulate phosphate homeostasis through transcriptional control of the renal type II sodium-dependent phosphate co-transporter (Npt2a and Npt2c) genes (2009)
  • Author(s): Masuda M, Yamamoto H, Tanaka S, Kozai M, Takei Y, Taketani Y, Miyamoto K, Takeda E
  • Organizer: 31th Annual Meeting of the Ametican Society for Bone and Mineral Research (ASBMR)
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 20090911-20090915
• [Presentation] Different Regulation of the Stanniocalcin Family (STC1 and STC2) Gene Expression by Parathyroid Hormone in the Renal Proximal Tubular Cells (2009)
  • Author(s): Takei Y, Yamamoto H, Nguyen Trong Hung, Masuda M, Taketani Y, Takeda E
  • Organizer: 31th Annual Meeting of the Ametican Society for Bone and Mineral Research (ASBMR)
  • Place of Presentation: Denver, Colorado, USA
  • Year and Date: 20090911-20090915
• [Presentation] 甲状腺ホルモン受容体β1を介したビタミンD合成酵素(CYP27b1)遺伝子の転写抑制機構 (2009)
  • Author(s): 香西美奈、山本浩範、竹井悠一郎、金子一郎、竹谷豊、宮本賢一、加藤茂明、武田英二
  • Organizer: 第61同日本ビタミン学会
  • Place of Presentation: 京都
  • Year and Date: 20090530-20090531
• [Presentation] ビタミンD一位水酸化酵素遣伝子(CYP27b1)の甲状腺ホルモン受容体β1を介した転写抑制機構 (2009)
  • Author(s): 香西美奈、山本浩範、増田真志、田中更紗、竹谷豊、宮本賢一、加藤茂明、武田英二
  • Organizer: 第63回日本栄養・食糧学会大会
  • Place of Presentation: 長崎
  • Year and Date: 20090520-20090522