2009 Fiscal Year Final Research Report
Induction of pyramidal neuronal cells from bone marrow stromal stemcells and application for autologous transplantation against the cerebral infarction.
| Project/Area Number |
20791019
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
NONOGUCHI Naosuke Osaka Medical College, 医学部, 非常勤講師 (70388263)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 骨髄幹細胞 / 神経分化 / 分化転換 / 神経再生 / 幹細胞移植 / 脳梗塞 / 錐体神経細胞 |
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| Research Abstract |
We demonstrated the way to induce the EACC1(glutamate transpoter)-positive pyramidal neuron-like cells from rat bone marrow stromal stem cells (BMSC). Transient hypoxia and cytokines stimulation following the genes transfection of Notch intracellular domain (NICD) and pituitary adenylate cyclase activating polypeptide (PACAP) promoted the transdifferentiation of BMSCs into the mutipolar neuron-like cells. One month after the transplantation of these cells into the infracted brain, some of recipient cells had survived in the peri-ischemic area of host brain and expressed the synapse-associated proteins.
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