2009 Fiscal Year Final Research Report
A Study of Dentin Regeneration via Mouse Embryonic Stem Cells
| Project/Area Number |
20791406
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
OZEKI Nobuaki Aichi Gakuin University, 歯学部, 助教 (70469005)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 歯内療法学 |
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| Research Abstract |
We have examined the mechanism of mouse embryonic stem cells differentiation to odontoblasts for the purpose of dentin regeneration. Morphological analysis indicated that retinoic acid (RA) and bone morphogenetic protein-4 (BMP-4) induced differentiation to the odontogenic pathway. Differentiated cells showed DSPP and DSP ; both markers of odontoblast differentiation. Moreover, we found significant integrin α2β1, α6β1 and αVβ1 expression with increased adhesion and motility on laminin-1 and collagen type-I substrates. These interactions with the extracellular matrix (ECM) are indicative of dental pulp wound healing.
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