The neural circuit of "male effect" pheromone signaling pathway in goat brain

2009 Fiscal Year Final Research Report

• Project/Area Number: 20880042
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: WAKABAYASHI Yoshihiro National Institute of Agrobiological Sciences, 脳神経機能研究ユニット, 任期付研究員 (00510695)
• Project Period (FY): 2008 – 2009
• Keywords: キスペプチン / 弓状核 / GnRH

Research Abstract

In goats, the pheromone produced by the male induces out of seasonal ovulation in anestrous females, the "male effect". This pheromonal information is transmitted to the hypothalamus via main and/or vomeronasal systems, although it remains unclear about this pathway. In this study, we used goats and we investigated about this pathway in the brain using morphological techniques. The medial amygdaloid nucleus (MeA) that is one of the major nuclei for transmission of pheromone signals, connect to kisspeptin neurons in the arcuate nucleus (ARC). This result suggests that signal of "male effect" pheromone is transmitted to kisspeptin neurons in the ARC, then evokes GnRH release.

Research Products

• [Journal Article] Neurokinin B and Dynorphin A in kisspept in neurons of the arcuate nucleus participate in generation of periodi c oscillation of neural activity driving pulsatile GnRH secretion in the goat. (2010)
  • Author(s): Wakabayashi Y, Nakada T, Murata K, Oh kura S, Mogi K, Navarro VM, Clifton DK, Mori Y, Tsukamura H, Maeda K-I, Steiner RA, Okamura H
  • Journal Title: The Journal of Neuroscience Vol.30 Pages: 3124-3132
  • Peer Reviewed
• [Journal Article] Modulation of GnRH Pulse Generator Activity by the Pheromone in Small Ruminants. (2009)
  • Author(s): Murata K., Wakabayashi Y., Kitago M., Ohara H., Watanabe H., Tamogami S., Warita Y., Yamagishi K., Ichikawa M., Takeuchi Y. Okamura H. Mori Y.
  • Journal Title: Journal of Neuroendocrinology Vol.21 Pages: 346-350
  • Peer Reviewed
• [Journal Article] Gonadotrophin-releasing hormone pulse generator activity in the hypothalamus of the goats. (2009)
  • Author(s): Ohkura S., Takase K., Matsuyama S., Mogi K., Ichimaru T., Wakabayashi Y., Uenoyama Y., Mori Y., Steiner RA., Tsukamura H., Maeda K-I., Okamura H.
  • Journal Title: Journal of Neuroendocrinology Vol.21 Pages: 813-821
  • Peer Reviewed
• [Presentation] Gonadotropin-releasing hormone pulse generator activity from the arcuate nucleus kisspeptin neurons in the goat hypothalamus (2010)
  • Author(s): Ohkura S., Wakabayashi Y., Uenoyama Y., Steiner RA., Tsukamura H., Maeda K-I., Okamura H.
  • Organizer: 14th International congress of endocrinology
  • Year and Date: 20100000
• [Presentation] ヤギ視床下部におけるキスペプチン/ Neurokinin B (NKB)/ Dynorphin A (Dyn)免疫陽性神経細胞の分布 (2009)
  • Author(s): 若林嘉浩・大蔵聡・村田健・森裕司・前多敬一郎・岡村裕昭
  • Organizer: 第24回下垂体研究会
  • Year and Date: 20090000
• [Presentation] シバヤギ弓状核キスペプチンニューロン神経活動に対するNeurokinin Bの作用 (2009)
  • Author(s): 若林嘉浩・大蔵聡・中田友明・村田健・Navarro VM・森裕司・束村博子・前多敬一郎・Steiner RA・岡村裕昭
  • Organizer: 第102回日本繁殖生物学会大会
  • Year and Date: 20090000
• [Presentation] ヤギオス効果フェロモンに対するGnRHパルスジェネレーターの反応性の解析 (2009)
  • Author(s): 村田健・若林嘉浩・坂本光平・大蔵聡・武内ゆかり・森裕司・岡村裕昭
  • Organizer: 第102回日本繁殖生物学会大会
  • Year and Date: 20090000
• [Presentation] 性腺ステロイドホルモンによる視床下部弓状核キスペプチンニューロンの制御 (2009)
  • Author(s): 坂本光平・若林嘉浩・村田健・大蔵聡・前多敬一郎・森裕司・岡村裕昭
  • Organizer: 第102回日本繁殖生物学会大会
  • Year and Date: 20090000
• [Presentation] シバヤギ弓状核キスペプチン神経機能の解析 (2009)
  • Author(s): 若林嘉浩
  • Organizer: 日本畜産学会第111回大会・若手企画シンポジウム
  • Year and Date: 20090000
• [Presentation] Neurokinin B as a cotransmitter in kisspeptin neurons in the regulation of pulsatile GnRH secretion (2009)
  • Author(s): Wakabayashi Y., Nakada T., Murata K., Ohkura S., Navarro V.M., Mori Y., Tsukamura H., Maeda K. -I., Steiner R.A., Okamura H.
  • Organizer: 39th Society for Neuroscience Annual meeting
  • Year and Date: 20090000
• [Presentation] Functional significance of dynorphin as a cotransmitter in kisspeptin neurons in the regulation of pulsatile GnRH secretion (2009)
  • Author(s): Ohkura S., Wakabayashi Y., Nakada T., Murata K., Navarro V.M., Mori Y., Tsukamura H., Maeda K. -I., Steiner R.A., Okamura H.
  • Organizer: 39th Society for Neuroscience Annual meeting
  • Year and Date: 20090000
• [Presentation] Kisspeptin neurons in the arcuate nucleus of goats receive neural projections from the medial nucleus of amygdala, a key nucleus for the pheromone signaling pathway (2008)
  • Author(s): Wakabayashi Y, Mogi K, Nakada T, Murata K, Ohkura S, Mori Y, Maeda K. I, Okamura H
  • Organizer: 1st World Conference on Kisspeptin Signaling in the Brain
  • Year and Date: 20080000
• [Presentation] Distribution of kisspeptin neurons in the shiba goat brain: An immunohistochmical study using two polyclonal antibody against different epitopes of goat kisspeptin 1st World (2008)
  • Author(s): Nakada T, Wakabayashi Y, Ohkura S, Maeda K. I, Okamura H
  • Organizer: Conference on Kisspeptin Signaling in the Brain
  • Year and Date: 20080000