2022 Fiscal Year Final Research Report
Functional analysis of novel Cas protein candidates for development of new genetic engineering technology
| Project/Area Number |
20H00413
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
白井 剛 長浜バイオ大学, バイオサイエンス学部, 教授 (00262890)
沼田 倫征 九州大学, 農学研究院, 准教授 (10401564)
跡見 晴幸 京都大学, 工学研究科, 教授 (90243047)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | クリスパー / 生体防御 / ゲノム編集 / ヌクレアーぜ / アーキア |
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| Outline of Final Research Achievements |
In this study, the principal investigator used a database of microbial metagenomic sequences in seawater, which he had created themselves, to extract sequences for CRISPR from it, and searched for protein-coding genes in the vicinity of the sequences to identify new CRISPR-Cas candidates. A total of 10 CRISPR-Cas candidates were found. The genes for the candidate Cas effector were cloned, and Cas proteins were produced in E. coli, and characterized them in vitro. The results of this study demonstrated that two types of miniature Cas proteins were identified with their crRNAs, PAM sequences, and cleave activity for the target genes.
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| Free Research Field |
農芸化学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果は、実施者が計画した戦略により、新規のCRISPR-Casを同定することができることを実験で証明したものであり、実際に本研究で見つかったものはゲノム編集技術に利用できる有用な資源となるので、ゲノム編集技術の幅が広がり、極めて社会的意義は大きい。また新規CRISPR-Casの性質を解析することは、CRISPR-Casの生理的な意義と作用機序の理解のためには貴重な材料となり、CRISPRの生物学の学術的発展に大きく貢献するものである。
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