Treatment of radiation damage in normal tissues using Muse cells and elucidation of the mechanism of radiation resistance of stem cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H03616
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 52040:Radiological sciences-related
• Research Institution: Tohoku University
• Principal Investigator: Hosoi Yoshio 東北大学, 医学系研究科, 教授 (50238747)
• Co-Investigator(Kenkyū-buntansha): 橋本 拓磨 東北大学, 医学系研究科, 講師 (50799145) ; 漆原 佑介 東北大学, 医学系研究科, 助教 (30722269)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 放射線 / 間葉系幹細胞

Outline of Final Research Achievements

The stability of Muse cells isolated as SSEA-3 positive cells from mesenchymal stem cells (MSCs) by FACS was investigated. It was found that when SSEA-3-positive cells were isolated and cultured, the proportion of SSEA-3-positive cells gradually decreased. It was also found that when SSEA-3-negative cells were isolated and cultured, SSEA-3-positive cells appeared, and the proportion of SSEA-3-positive cells was similar to that before isolation. In addition, when SSEA-3 positive cells were isolated from MSCs and cultured for 36 days in a conditioned medium that increases stemness, the SSEA-3 positive rate was 4.56%. This value was lower than the SSEA-3 positive rate of 19.45% when cultured in a control medium for 36 days.

Free Research Field

放射線生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、臨床試験が進行しているMuse細胞を用いて、Muse細胞の多分化能を高めて正常組織の放射線障害の治療に役立てるために研究を行った。しかし、Muse細胞と間葉系幹細胞（MSC）には互換性があり、MSC中に一定の割合でMuse細胞が発現するような機構が存在する事が示唆された。また、Muse細胞を高める調整培地で培養すると、コントロール培地で培養する場合に比べ、use細胞の比率は低下する事が明らかになった。これらのことから、Muse細胞はMSCと同等かやや分化が進んだ細胞であることが示唆された。