2022 Fiscal Year Final Research Report

Investigation of the mechanism of ortho- and non-keratinization pattern using different stiffness of scaffold and its application for fabrication of tissue-engineered oral mucosa

Research Project

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Project/Area Number	20H03870
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 57040:Regenerative dentistry and dental engineering-related
Research Institution	Niigata University
Principal Investigator	Izumi Kenji 新潟大学, 医歯学系, 教授 (80242436)
Co-Investigator(Kenkyū-buntansha)	芳賀永北海道大学, 先端生命科学研究院, 教授 (00292045) 石原誠一郎北海道大学, 先端生命科学研究院, 助教 (10719933) 加来賢新潟大学, 医歯学系, 准教授 (30547542) 佐藤大祐新潟大学, 研究推進機構, 助教 (70778703) 凌一葦新潟大学, 医歯学系, 助教 (70804540) 鈴木絢子新潟大学, 医歯学系, 特任助教 (70869916)
Project Period (FY)	2020-04-01 – 2023-03-31
Keywords	メカノバイオロジー / 口腔粘膜 / 口腔粘膜角化細胞 / 正角化 / 非角化 / 培養口腔粘膜
Outline of Final Research Achievements	Although histology of oral mucosa often demonstrates dramatic change of keratinization pattern, for instance, at muco-gingival fold. However, the mechanism is not well-elucidated. This study aimed to investigate how the histological differences are regulated in terms of mechanobiology. Using human primary oral keratinocytes culturing on two types of collagen matrices with different stiffness and regular culture dishes, we examined micro-array analyses, histological observation of tissue-engineered oral mucosa and measurement of Young’s modulus. The gene expression analysis revealed that there are many genes that are up- and down-regulated depending on the stiffness. However, there are few genes specific to biological functions of oral keratinocytes. Furthermore, there were scarce differences of histological appearances and Young's modulus among cells grown on matirces with different stiffness. It may be required to increase the number of samples and matrix stiffness to be examined.
Free Research Field	再生歯学
Academic Significance and Societal Importance of the Research Achievements	本研究の結果から、口腔粘膜上皮角化細胞の分化・増殖という特徴的な事象は、足場材の硬さの違いによっても大いに制御されていることを示すと考えられた。特に口腔と言う器官は非常に複雑な解剖学的構造を呈していることから、今後口腔粘膜の再生医療を推進していく上で、メカノトランスダクション機構による細胞応答反応の検討は不可欠であることが示唆される。