Development of two-dimensional ionomics and its application to plant nutrition

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K05762
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38010:Plant nutrition and soil science-related
• Research Institution: Hokkaido University
• Principal Investigator: Watanabe Toshihiro 北海道大学, 農学研究院, 准教授 (60360939)
• Co-Investigator(Kenkyū-buntansha): 平舘 俊太郎 九州大学, 農学研究院, 教授 (60354099)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: イオノミクス / 植物 / 土壌

Outline of Final Research Achievements

The aim of this study was to establish a two-dimensional analysis method for developing ionomics, a comprehensive elemental analysis method, by taking into account the differences in the chemical form of each element. Two two-dimensional elemental profiling methods were devised for plants, one based on fractionation and the other based on extraction with different extractants. These were applied to the study of elemental dynamics in the response to excess mineral stress in plants, and to the study of fruit ionome variation due to differences in the incidence of blossom-end rot among different tomato lines, to detect variation that could not be detected by total mineral analysis. Two-dimensional elemental profiling of soils with different extractants was also carried out, showing their potential application to the influence of plant roots on rhizosphere elemental dynamics and to the detailed estimation of elemental availability at different soil pH.

Free Research Field

植物栄養学

Academic Significance and Societal Importance of the Research Achievements

イオノミクスは植物栄養学では標準的な手法として近年広く使われているが、その発展のために各元素の形態に注目した解析は重要である。本研究は植物組織あるいは土壌に存在する各元素を一斉分画し、異なる形態・存在状態ごとに元素プロファイル得るという画期的な手法を提案した。この手法は作物栽培では作物-土壌間の元素動態（養分元素、有害元素）を明らかにすることに、また無機元素に起因する作物の生理障害への対策に貢献することが期待される。