Real-time observation of lectin cross-linking reactions by multivalent carbohydrates and new approach to ligand design

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K05855
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38040:Bioorganic chemistry-related
• Research Institution: Fukushima University
• Principal Investigator: Ogata Makoto 福島大学, 食農学類, 准教授 (10532666)
• Co-Investigator(Kenkyū-buntansha): 若松 孝 茨城工業高等専門学校, 国際創造工学科, 教授 (80220838)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 糖鎖 / レクチン / 凝集 / 糖鎖クラスター / 架橋反応 / 前方静的光散乱 / 分子認識

Outline of Final Research Achievements

We present a real-time observation and a structurally variable process of the cross-linking agglutination between multivalent lectins and glycoclusters using a small-angle forward static light scattering (F-SLS) technique. In this study, a cross-linking agglutination reaction was carried out using Sambucus Sieboldiana agglutinin (SSA) and a tetravalent Neu5Acα2,6LacNAc-glycocluster as a cross-linker. The scattering intensity of time-resolved F-SLS increased with the formation of the Neu5Acα2,6LacNAc-glycocluster－SSA cross-linked complex. As a result, we succeeded in observing the fine sequential cross-linking agglutination phenomenon between the multivalent carbohydrates and the multivalent lectins in real time with F-SLS. Furthermore, the structural analysis based on the fractal dimension using the time-resolved F-SLS pattern revealed that the structure of the aggregates changes densely as the cross-linking reaction progresses, and that the process is an equilibrium reaction.

Free Research Field

生物有機化学

Academic Significance and Societal Importance of the Research Achievements

ナノからサブミクロンスケールで変化する糖鎖と多価レクチン間での凝集体形成反応を、これら凝集体の分析に高感度な独自のF-LS瞬時測定技術によって明らかにした。これら一連の成果は、これまで不可能であった多価糖鎖と多価レクチン間架橋反応のリアルタイム観察を可能にした点で、学術的意義は非常に高い。また、これら相互作用はウイルスの宿主感染の際にもみられる普遍的な結合原理であるため、本技術を病原性ウイルスの検出や捕捉に応用展開が可能であり、社会的意義も高いと考えられる。