Identification of chondroitin sulfate isotypes with adipocyte differentiation inhibitory activity, elucidation of their functions, and regulation of fat mass

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K06411
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Kyushu University (2022); Tottori University (2020-2021)
• Principal Investigator: HOSAKA Yoshinao 九州大学, 農学研究院, 教授 (00337023)
• Co-Investigator(Kenkyū-buntansha): 田村 純一 鳥取大学, 農学部, 教授 (30221401) ; 割田 克彦 鳥取大学, 農学部, 教授 (40452669) ; 北村 直樹 鳥取大学, 農学部, 准教授 (80301951)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 脂肪細胞分化 / コンドロイチン硫酸 / 糖鎖量 / 糖鎖の構成割合 / 糖鎖長 / 3T3-L1 / 細胞外マトリックス

Outline of Final Research Achievements

Focusing on the relationship between adipocyte differentiation and chondroitin sulfate (CS), the following experiments were conducted. 1) Induced the differentiation of progenitor adipocyte 3T3-L1 into adipocytes in a medium containing CS, and attempted to identify CS species with high adipogenic differentiation inhibitory activity. As a result, C type rich CS showed strong inhibitory activity against adipogenesis. 2) Analyzed in detail the changes in CS chains before and after differentiation into adipocytes, and found that the amount of chains, the CS species constituting them, and their proportions changed. Furthermore, the average chain length also became shorter after differentiation, indicating that qualitative and quantitative changes in the CSs surrounding the cells occurred with the progress of adipocyte differentiation.

Free Research Field

糖鎖生物学

Academic Significance and Societal Importance of the Research Achievements

細胞外マトリックスとして存在するCSが、その質や量（構成する糖鎖の種類、割合、糖鎖長など）を絶妙に変化させて、脂肪細胞の分化を制御していることを本課題で、初めて詳細に確認し、報告した。得られた成果の応用、すなわち、CSの量や質を部位・時期特異的に変化させる技術を開発することで、将来、脂肪細胞の分化抑制、すなわち脂肪量の人為的制御が可能になると考えられる。