Elucidating molecular pathogenesis of canine prostate cancer focused on micro RNA

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K06439
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: Kobayashi Masanori 日本獣医生命科学大学, 獣医学部, 准教授 (80600428)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 犬 / 前立腺癌 / microRNA

Outline of Final Research Achievements

In this study, three canine prostate cancer cell lines were used to examine the cell proliferation suppressive effect in vitro using inhibitors of mir-18a, 95, 221, and 330, which are upregulated in cancer tissues. The results showed that mir-330 inhibitor had a high ability to inhibit cell proliferation in all of the cell lines. In addition, in order to identify the genes targeted by mir-330, we focused on the tumor suppressor genes PTPN9 and SPRY2 and performed mRNA expression analysis. The mRNA expression level of PTPN9 was significantly increased in all cells compared to the control group. On the other hand, although the expression level of SPRY2 was increased in all cells, the change in expression was not significant. Furthermore, the mRNA expression levels of apoptosis-related markers increased after the addition of mir-330 inhibitor. Mir-330 inhibitor was suggested to induce apoptosis in canine prostate cancer cells through regulation of PTPN9 expression.

Free Research Field

獣医臨床繁殖学

Academic Significance and Societal Importance of the Research Achievements

本研究ではmir-330が癌抑制遺伝子であるPTPN9の発現調節を通じて、犬前立腺癌の増殖に寄与することを示唆した。これらの知見は未だ著効を示す治療が確立されていない犬前立腺癌に対するmir-330またはPTPN9を標的とした新たな分子標的治療戦略の基盤をなすだけでなく、同様の制御機構をもつ癌治療に対しても有益な知見を提供しうるものであり社会的な意義があるだろう。