2023 Fiscal Year Final Research Report

Novel and rapid diagnostic appproach of AL amyloidosis by Raman spectroscopy

Research Project

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Project/Area Number	20K08712
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 54010:Hematology and medical oncology-related
Research Institution	The University of Tokushima
Principal Investigator	MIKI Hirokazu 徳島大学, 病院, 講師 (50511333)
Co-Investigator(Kenkyū-buntansha)	南川丈夫徳島大学, ポストLEDフォトニクス研究所, 准教授 (10637193) 高成広起徳島大学, ポストLEDフォトニクス研究所, 准教授 (70723253)
Project Period (FY)	2020-04-01 – 2024-03-31
Keywords	ALアミロイドーシス / ラマン分光法 / 心臓アミロイドーシス / 主成分分析
Outline of Final Research Achievements	Systemic AL amyloidosis remains a life-threatening disease that involves multi-organs including heart. The diagnosis of cardiac amyloidosis is often delayed, due to the non-specificity of clinical symptoms. Raman spectroscopy is a label-free and rapid technique that can provide structural information of a target, being adapted for biomedical analysis. Here, we explored the diagnostic utility of amyloidosis by Raman spectroscopy. Fifteen pathological specimens from 11 patients (8 AL, 2 ATTR, 1 AA) were examined. Raman spectroscopy analysis showed a specific peak of Raman shift at 1680 cm-1 in the amyloid deposited areas, which indicative of the structure of β-sheet. Interestingly, a principal component analysis of the spectra showed the differences in cardiac biopsy specimens between AL and ATTR amyloidosis. These results suggest that Raman spectroscopy may contribute to a rapid differential diagnosis in patients with amyloidosis, especially in cardiac amyloidosis.
Free Research Field	血液内科学
Academic Significance and Societal Importance of the Research Achievements	全身性アミロイドーシスは、臨床症状が非特異的であり、また初発症状から診断までに1年以上を要する症例も多い。特に心臓アミロイドーシスは心不全や不整脈などで生命予後不良である。本研究では、ラマン分光法を用いてアミロイドーシスの迅速診断を試みた。本研究結果から、ラマン分光法によるアミロイドーシスに対する新規迅速診断法の確立は，難病である全身性アミロイドーシス，および致死性の高い心臓アミロイドーシスの早期診断，治療開始の迅速化に寄与する可能性があり，臨床への貢献度は非常に大きいと考えられる。