2021 Fiscal Year Annual Research Report
Elucidating plasma effect on structural modification of antioxidant enzymes: Combined experimental and computational
| Project/Area Number |
20K14454
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
Attri Pankaj 九州大学, プラズマナノ界面工学センター, 准教授 (40868361)
|
|---|
| Project Period (FY) |
2020-04-01 – 2023-03-31
|
| Keywords | Computational study / Mdm2 / p53 / Biophysical techniques |
|---|
| Outline of Annual Research Achievements |
The discharge under atmospheric conditions is also commonly known as cold atmospheric plasma (CAP). CAP generates plenty of reactive chemical species, both short and long-lived reactive species. In the last few years, CAP use in cancer treatment has increased tremendously. Besides the reported achievements of CAP to treat cancer, CAP’s action is still not known entirely. Several early studies focused on the murine double minute 2 (Mdm2)& p53(tumor suppressor protein). However, the effect of CAP treatment on Mdm2 and p53 is absent from the literature from long time. In our study we investigated the structural changes in Mdm2, p53, and the Mdm2-p53 before and after possible plasma oxidation through molecular dynamic (MD) simulations. MD calculation revealed that the oxidized Mdm2 bounded or unbounded showed high flexibility that might increase the availability of tumor suppressor protein p53 in plasma-treated cells.
|
