A fluorogenic click reaction for rapid quantitative analysis

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K15952
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 47010:Pharmaceutical chemistry and drug development sciences-related
• Research Institution: University of Toyama
• Principal Investigator: Kohyama Aki 富山大学, 学術研究部薬学・和漢系, 助教 (40778586)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: オルトキノジメタン / アジド / ホスフィン / Staudinger / 2分子連結

Outline of Final Research Achievements

A Ligation reaction forming a fluorophore is one of important tools for biological study and drug development. In this work, we aimed to develop such a reaction by refining a ligation reaction reported by our group. Then, we designed the reaction and substrates and carried out the plan. As a result, our experiments proved that a desired aromatization, a crucial step to form fluorophore, did not proceed. However, we could improve yield of the original ligation reaction and find a novel ligation reaction unexpectedly.

Free Research Field

生物有機化学

Academic Significance and Societal Importance of the Research Achievements

本研究課題では、所望の反応ではないものの、新しい２成分連結反応が見出された。このような温和な条件下での連結反応は、将来、生命現象の解明や医薬品創出のための道具として発展していくことが期待される。したがって、本研究成果は学術的にも医薬学への波及効果的にも意義深いものと位置付けられる。