2020 Fiscal Year Research-status Report
Distinct roles of IL-27 produced by macrophages and dendritic cells in shaping the immune response against Plasmodium parasites
| Project/Area Number |
20K16236
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| Research Institution | Nagasaki University |
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Principal Investigator |
バヤルサイハン ガンチメグ 長崎大学, 医歯薬学総合研究科(医学系), 助教 (80841353)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | Interleukin 27 / malaria / macrophage / dendritic cells |
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| Outline of Annual Research Achievements |
Mice lacking IL-27 in DCs (IL27p28fl/flCD11c-Cre) or in macrophages (IL27p28fl/flLysM-Cre) were infected compared the response to the infection with P.chabaudi. Mice lacking IL-27 in DCs showed delay in the clearance of parasitemia than that in control mice (IL27p28fl/fl) during the acute phase of the infection, while those in macrophages exhibited earlier parasitemia peak and earlier clearance. Frequencies of activated CD4+ T cells CD11ahiCD4
9dhi) and IFN-γ producing CD8+ T cells were higher in mice lacking IL-27 in DCs than those in control mice, while IFN-γ/TNF-α double producing CD4+ T cells were higher in mice lacking IL-27 in macrophages. We have also observed better antibody production in mice IL-27 in DCs than those in control mice and in mice lacking IL-27 in macrophages.These results suggest that IL-27 produced by DC and macrophages play qualitatively differential roles in the immune responses against Plasmodium infection. To confirm functional necessity of IL-27 produced by DCs and macrophages for disease control and development of immune memory against Plasmodium infection, following experiments will be done in the future. To test recall responses, groups of mice will be rechallenged with the heterologous parasite after complete clearance of the parasite. Mice will be monitored for parasitemia and disease progress after rechallenge. Expansion of memory cells in the spleen and their function will be checked after therechallenge.
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| Current Status of Research Progress |
Current Status of Research Progress
4: Progress in research has been delayed.
Reason
I have been on maternity leave since December 2020. I am planning to start working from October 2021.
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| Strategy for Future Research Activity |
To confirm functional necessity of IL-27 produced by DCs and macrophages for disease control and development of immune memory against Plasmodium infection, following experiments will be done in the future. To test recall responses, groups of mice will be rechallenged with the heterologous parasite after complete clearance of the parasite. Mice will be monitored for parasitemia and disease progress after rechallenge. Expansion of memory cells in the spleen and their function will be checked after the rechallenge.
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| Causes of Carryover |
I took leave from December 2020 and the research has been stopped since then. I have a leftover balance from fiscal year 2020.
I need to purchase fluorescent antibodies, disposables and need to maintain conditional knockout mice for my experiments. Fluorescent-labeled antibodies will be used for flow cytometry and immunohistochemistry, these are the fundamental assays in my research. Purified mouse cytokines for ELISA will be purchased. Other reagents and chemicals required for immunological assays and cell cultures must be purchased. Disposable laboratory tools and plastic wares will be used often in daily research assays.
Travel expenses are estimated for two domestic travels in each year for around 3-4 days accommodation in each trip. The purpose of the trip is attending and presenting my new data in the meeting of Japanese Society of Immunology (JSI) and Japanese Society of Parasitology (JSP). Overseas travel expense is estimated for 4-5 days accommodation and round trip flights. The purpose of trip is to presenting new data and networking with international scientists in the annual meeting International Congress of Immunology.
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