2022 Fiscal Year Final Research Report
Investigation of liver kinetics in humanized liver mice and establishment of microimaging biomarkers
Project/Area Number |
20K16807
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 52040:Radiological sciences-related
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Research Institution | Jikei University School of Medicine (2021-2022) Central Institute for Experimental Animals (2020) |
Principal Investigator |
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | MRI / Liver |
Outline of Final Research Achievements |
In this study, high-resolution multi-contrast images were obtained using 7T-MRI in wild-type and humanized liver mice. To establish accurate liver function imaging, parameters were adjusted to detect the movement of water molecules at the hepatocyte level in multiple widths and directions, and specific directional anisotropy of peri-hepatocyte water molecules was demonstrated. Furthermore, we found that a sufficiently high b-value is necessary for predicting liver function from water molecule diffusion information in living liver tissue, taking into account the effect of perfusion. Humanized liver in mice showed diffuse T2 prolongation and increased diffusion coefficient. As the first imaging study in humanized liver mice, we were able to elucidate the details of humanized liver.
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Free Research Field |
MRI
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Academic Significance and Societal Importance of the Research Achievements |
臨床においては評価が難しい、本質的な肝細胞レベルでの水分子の拡散の影響を評価することができた。さらに、肝臓そのものがヒトの肝臓に取って代わるヒト化肝臓マウスにおいて、初めてのイメージング研究として、ヒト化肝臓の詳細を明らかにすることができた。本結果は、肝細胞変性など、肝疾患のイメージング評価の基礎となるデータである。さらに、ヒト化肝臓モデルをイメージングとして、構造、機能を明らかにすることで、創薬などの臨床へのフィードバックへの貢献が十分に期待できる。
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