Potential of cell transplantation with bronchoalveolar stem cells derived from iPS cells for lung regeneration.

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K17751
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55040:Respiratory surgery-related
• Research Institution: The University of Tokushima
• Principal Investigator: KAWAKITA Naoya 徳島大学, 病院, 講師 (60522266)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 再生医療 / iPS細胞 / 幹細胞 / 細胞移植 / オルガノイド

Outline of Final Research Achievements

Functional analysis of iPS-derived bronchoalveolar stem cells (BASCs) is ongoing, and organoid cultures of a group of BASCs cells differentiated from iPS cells and sorted by the surface markers Sca-1/CD45/CD31 were performed. The cultures showed the formation of organoids with various morphologies, similar to previously reported BASCs derived from mouse lungs. Further immunostaining and flow cytometric analysis of the organoids formed showed that iPS-derived BASCs had self-renewal potential and differentiated into various epithelial cells in the bronchiolar region.

Free Research Field

呼吸器外科学

Academic Significance and Societal Importance of the Research Achievements

iPS由来のBASCsが生体内同様に多能性を持つことが示された。これまでiPS由来のBASCsの経気道投与が、気道上皮障害の修復を促進することは明らかとなっていたが、その機能解析がなされたことで、iPS由来BASCsが生体内BASCsに近い分化能を有する可能性が示唆されており、修復促進の機序の一因と考えられた。