A Novel Mechanism of Genetic Mutation Independent Metal Tolerance in a Multi-Hierarchical Terrestrial Organism Population Using High Precision XAFS

2021 Fiscal Year Research-status Report

• Project/Area Number: 20K20633
• Research Institution: Hokkaido University
• Principal Investigator: 中山 翔太 北海道大学, 獣医学研究院, 准教授 (90647629)
• Co-Investigator(Kenkyū-buntansha): 藤森 崇 龍谷大学, 先端理工学部, 准教授 (20583248) ; 中田 北斗 北海道大学, 獣医学研究院, 博士研究員 (60815273) [Withdrawn]
• Project Period (FY): 2020-07-30 – 2023-03-31
• Keywords: 金属耐性機構 / 動物種差 / XAFS解析 / 野生動物 / 毒性発現 / カブウェ鉱床

Outline of Annual Research Achievements

野生ラットの臓器および内容物の鉛化学形態分布について、Pb L3端 XANESスペクトルによる解析を進めた。高濃度の鉛で汚染されたラットであることに加えて、凍結乾燥による試料均一性の増大や鉛濃度の濃縮によって、これまで鉛低濃度により測定が制約されていた生物試料のXANESスペクトルの臓器別、内容物別の測定に成功した。野生ラット3個体を主として、臓器は心臓、脳、肺、脾臓、肝臓、腎臓、筋肉など、胃・小腸・大腸の内容物、および糞便に対して、スペクトルの質を考慮したうえで系統的な解析を試みた。鉛化学形態の参照用XANESとして、グルタチオンと鉛の結合体や経口曝露源と考えられる現地の鉛汚染土壌等のデータを用いた。スペクトル形状および参照スペクトルとの線形結合フィッティングの結果、臓器別の鉛形態の違いや、消化器系や体内へ吸収された際の鉛化学形態の変化に関する知見を得ることができた。トカゲ亜目(Trachylepis wahlbergii)計112匹の臓器（肝臓、肺、卵巣/精巣）、血液、胃内容物を採集し肝臓中の金属濃度の測定を行った。土壌、トカゲ臓器、生体内で鉛と結合することが知られているグルタチオンなどと鉛の結合試料のPb L3端 XANESスペクトルによる解析を行った。鉛の周囲にグルタチオンをはじめ硫黄化合物が存在することが示唆された。このため、硫黄K端のエネルギー領域も計測し、個体間及び臓器間で異なるスペクトルパターンを得た。化学形態に加えて多角的なデータの取得を目的に遺伝子解析も実施しデータベース上にない全ゲノム配列データを得た。肝臓、肺、卵巣ついてRNA-seqも実施した。関係省庁・現地獣医師の協力の下、ニワトリ（10個体）、イヌ（26個体）の死亡個体の肝臓、腎臓、骨、腸管、脳、心臓、脾臓を採材し、鉛など10元素の濃度測定も行った。ラットの動物実験による確認実験も実施している。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

予定通り採材、解析を実施しており、成果発表も充分に実施しているため。

Strategy for Future Research Activity

今後は、今年度に引き続きXAFS解析、LA-ICP-MS解析などを行う予定である。遺伝子解析やエピジェネティクス解析も同じ個体から行うことで、遺伝子変異以外の金属耐性機構のメカニズム解明を行っていく予定である。

Causes of Carryover

コロナウイルス感染症により予定していた海外渡航およびこれに関わるサンプリングの謝金支出が大幅に減額されたため。次年度には予定通り海外渡航を行う予定である。

Research Products

• [Int'l Joint Research] ザンビア大学/ザンビア保健省/ザンビア畜産水産省(ザンビア)
  • Country Name: ZAMBIA
  • Counterpart Institution: ザンビア大学/ザンビア保健省/ザンビア畜産水産省
  • # of Other Institutions: 3
• [Int'l Joint Research] ナミビア大学(ナミビア)
  • Country Name: NAMIBIA
  • Counterpart Institution: ナミビア大学
• [Journal Article] Human Health Risk Assessment from Lead Exposure through Consumption of Raw Cow Milk from Free-Range Cattle Reared in the Vicinity of a Lead?Zinc Mine in Kabwe (2022)
  • Author(s): Zyambo Golden、Yabe John、Muzandu Kaampwe、M’kandawire Ethel、Choongo Kennedy、Kataba Andrew、Chawinga Kenneth、Liazambi Allan、Nakayama Shouta M. M、Nakata Hokuto、Ishizuka Mayumi
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 19 Pages: 4757～4757
  • DOI: 10.3390/ijerph19084757
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Alkaline Leaching and Concurrent Cementation of Dissolved Pb and Zn from Zinc Plant Leach Residues (2022)
  • Author(s): Silwamba Marthias、Ito Mayumi、Hiroyoshi Naoki、Tabelin Carlito Baltazar、Hashizume Ryota、Fukushima Tomoki、Park Ilhwan、Jeon Sanghee、Igarashi Toshifumi、Sato Tsutomu、Nyambe Imasiku、Nakata Hokuto、Nakayama Shouta、Ishizuka Mayumi
  • Journal Title: Minerals Volume: 12 Pages: 393～393
  • DOI: 10.3390/min12040393
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Geochemical identification of particulate lead pollution in shallow groundwater in inhabited areas in Kabwe, Zambia (2022)
  • Author(s): Toyoda Kazuhiro、Nakano Shota、Tanaka Shunitz、Banda Kawawa、Nyambe Imasiku A.、Ishikawa Tsuyoshi、Nakayama Shouta、Ishizuka Mayumi
  • Journal Title: Applied Geochemistry Volume: 139 Pages: 105215～105215
  • DOI: 10.1016/j.apgeochem.2022.105215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metabolomic Alteration in the Plasma of Wild Rodents Environmentally Exposed to Lead: A Preliminary Study (2022)
  • Author(s): Nakata Hokuto、Eguchi Akifumi、Nakayama Shouta M. M.、Yabe John、Muzandu Kaampwe、Ikenaka Yoshinori、Mori Chisato、Ishizuka Mayumi
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 19 Pages: 541～541
  • DOI: 10.3390/ijerph19010541
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Geospatial approach to investigate spatial clustering and hotspots of blood lead levels in children within Kabwe, Zambia (2022)
  • Author(s): Moonga Given、Chisola Moses N.、Berger Ursula、Nowak Dennis、Yabe John、Nakata Hokuto、Nakayama Shouta、Ishizuka Mayumi、Bose-O'Reilly Stephan
  • Journal Title: Environmental Research Volume: 207 Pages: 112646～112646
  • DOI: 10.1016/j.envres.2021.112646
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Environmentally relevant lead (Pb) water concentration induce toxicity in zebrafish (Danio rerio) larvae (2022)
  • Author(s): Kataba Andrew、Botha Tarryn L.、Nakayama Shouta M.M.、Yohannes Yared B.、Ikenaka Yoshinori、Wepener Victor、Ishizuka Mayumi
  • Journal Title: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Volume: 252 Pages: 109215～109215
  • DOI: 10.1016/j.cbpc.2021.109215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Solid-Phase Partitioning and Leaching Behavior of Pb and Zn from Playground Soils in Kabwe, Zambia (2021)
  • Author(s): Mufalo Walubita、Tangviroon Pawit、Igarashi Toshifumi、Ito Mayumi、Sato Tsutomu、Chirwa Meki、Nyambe Imasiku、Nakata Hokuto、Nakayama Shouta、Ishizuka Mayumi
  • Journal Title: Toxics Volume: 9 Pages: 248～248
  • DOI: 10.3390/toxics9100248
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of zinc on tissue uptake and toxicity of lead in Sprague Dawley rat (2021)
  • Author(s): KATABA Andrew、NAKAYAMA Shouta M.M.、YOHANNES Yared Beyene、TOYOMAKI Haruya、NAKATA Hokuto、IKENAKA Yoshinori、ISHIZUKA Mayumi
  • Journal Title: Journal of Veterinary Medical Science Volume: 83 Pages: 1674～1685
  • DOI: 10.1292/jvms.20-0684
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Glutathione S-transferase gene polymorphisms in association with susceptibility to lead toxicity in lead- and cadmium-exposed children near an abandoned lead-zinc mining area in Kabwe, Zambia (2021)
  • Author(s): Yohannes Yared Beyene、Nakayama Shouta M. M.、Yabe John、Toyomaki Haruya、Kataba Andrew、Nakata Hokuto、Muzandu Kaampwe、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Environmental Science and Pollution Research Volume: 29 Pages: 6622～6632
  • DOI: 10.1007/s11356-021-16098-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of Dispersion of Lead-Bearing Mine Wastes in Kabwe District, Zambia (2021)
  • Author(s): Nakamura Shinsaku、Igarashi Toshifumi、Uchida Yoshitaka、Ito Mayumi、Hirose Kazuyo、Sato Tsutomu、Mufalo Walubita、Chirwa Meki、Nyambe Imasiku、Nakata Hokuto、Nakayama Shouta、Ishizuka Mayumi
  • Journal Title: Minerals Volume: 11 Pages: 901～901
  • DOI: 10.3390/min11080901
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lead concentrations and isotope ratios in blood, breastmilk and feces: contribution of both lactation and soil/dust exposure to infants in a lead mining area, Kabwe, Zambia (2021)
  • Author(s): Toyomaki Haruya、Yabe John、Nakayama Shouta M.M.、Yohannes Yared B.、Muzandu Kaampwe、Mufune Tiza、Nakata Hokuto、Ikenaka Yoshinori、Kuritani Takeshi、Nakagawa Mitsuhiro、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Environmental Pollution Volume: 286 Pages: 117456～117456
  • DOI: 10.1016/j.envpol.2021.117456
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome-wide DNA methylation analysis of dogs with high lead exposure living near a lead mining area in Kabwe, Zambia (2021)
  • Author(s): Yamazaki Jumpei、Toyomaki Haruya、Nakayama Shouta M.M.、Yabe John、Muzandu Kaampwe、Jelinek Jaroslav、Yokoyama Shoko、Ikenaka Yoshinori、Takiguchi Mitsuyoshi、Ishizuka Mayumi
  • Journal Title: Environmental Pollution Volume: 286 Pages: 117229～117229
  • DOI: 10.1016/j.envpol.2021.117229
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The impact of elevated blood lead levels in children on maternal health-related quality of life (2021)
  • Author(s): Nakata Hokuto、Tohyama Harukazu、Fujita Wakako、Nakayama Shouta M.M.、Ishizuka Mayumi、Yabe John、Munyinda Nosiku S.、Sakala Doreen、Choongo Kennedy、Yamasaki Shojiro、Nagai Natsumi、Yoshida Takahiko、Saito Takeshi
  • Journal Title: Chemosphere Volume: 279 Pages: 130490～130490
  • DOI: 10.1016/j.chemosphere.2021.130490
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Analysis of lead distribution in avian organs by LA-ICP-MS: Study of experimentally lead-exposed ducks and kites (2021)
  • Author(s): Torimoto Ryouta、Ishii Chihiro、Sato Hiroshi、Saito Keisuke、Watanabe Yukiko、Ogasawara Kohei、Kubota Ayano、Matsukawa Takehisa、Yokoyama Kazuhito、Kobayashi Atsushi、Kimura Takashi、Nakayama Shouta M.M.、Ikenaka Yoshinori、Ishizuka Mayumi
  • Journal Title: Environmental Pollution Volume: 283 Pages: 117086～117086
  • DOI: 10.1016/j.envpol.2021.117086
  • Peer Reviewed / Open Access
• [Journal Article] Assessment of LeadCare II analysis for testing of a wide range of blood lead levels in comparison with ICP-MS analysis (2021)
  • Author(s): Nakata Hokuto、Nakayama Shouta M.M.、Yabe John、Muzandu Kaampwe、Toyomaki Haruya、Yohannes Yared Beyene、Kataba Andrew、Zyambo Golden、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Chemosphere Volume: 271 Pages: 129832～129832
  • DOI: 10.1016/j.chemosphere.2021.129832
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An Investigation of the Wild Rat Crown Incisor as an Indicator of Lead (Pb) Exposure Using Inductively Couple Plasma Mass Spectrometry (ICP-MS) and Laser Ablation ICP-MS (2021)
  • Author(s): Kataba Andrew、Nakayama Shouta M. M.、Nakata Hokuto、Toyomaki Haruya、Yohannes Yared B.、Yabe John、Muzandu Kaampwe、Zyambo Golden、Kubota Ayano、Matsukawa Takehisa、Yokoyama Kazuhito、Ikenaka Yoshinori、Ishizuka Mayumi
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 18 Pages: 767～767
  • DOI: 10.3390/ijerph18020767
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of the ameliorative effect of Spirulina (Arthrospira platensis) supplementation on parameters relating to lead poisoning and obesity in C57BL/6J mice (2021)
  • Author(s): Nakata Hokuto、Nakayama Shouta M.M.、Kataba Andrew、Yohannes Yared Beyene、Ikenaka Yoshinori、Ishizuka Mayumi
  • Journal Title: Journal of Functional Foods Volume: 77 Pages: 104344～104344
  • DOI: 10.1016/j.jff.2020.104344
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Delta-aminolevulinic acid dehydratase (ALAD) and vitamin D receptor (VDR) genes polymorphisms in children residing in an abandoned lead/zinc mine area in Kabwe, Zambia (2021)
  • Author(s): Yohannes Yared B.、Nakayama Shouta M.M.、Yabe John、Toyomaki Haruya、Kataba Andrew、Nakata Hokuto、Muzandu Kaampwe、Ikenaka Yoshinori、Choongo Kennedy、Ishizuka Mayumi
  • Journal Title: Meta Gene Volume: 27 Pages: 100838～100838
  • DOI: 10.1016/j.mgene.2020.100838
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Lead concentrations and isotope ratios in blood, breastmilk and feces: contribution of both lactation and soil/dust exposure to infants in a lead mining area, Kabwe, Zambia (2021)
  • Author(s): Shouta M.M. Nakayama, Haruya Toyomaki, John Yabe, Yared Beyene Yohannes, Kaampwe Muzandu, Tiza Mufune, Hokuto Nakata, Yoshinori Ikenaka, Takeshi Kuritani, Mitsuhiro Nakagawa, Kennedy Choongo, Mayumi Ishizuka
  • Organizer: SOT2022
• [Presentation] Land Use in Habitats Affects Metal Concentrations in Wild Lizards Around a Former Lead Mining Site (2021)
  • Author(s): Nakayama SMM, Doya R, Nakata H, Toyomaki H, Yabe J, Muzandu K, Yohannes Y, Kataba A, Zyambo G, Ogawa T, Uchida Y, Ikenaka Y, Ishizuka M
  • Organizer: SETAC Europe 31st Annual Meeting
  • Int'l Joint Research
• [Presentation] Investigation of major sources of human exposure and estimation of exposure reduction effects by environmental remediation using IEUBK model in lead-contaminated area (2021)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, John Yabe, Kaampwe Muzandu, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第29回環境化学討論会
• [Presentation] Evaluation of the mitigative effect of Spirulina (Arthrospira platensis) supplementation on lead poisoning and obesity in C57BL/6J mice (2021)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Andrew Kataba, Yared B. Yohannes, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 第29回環境化学討論会
• [Presentation] Investigation of human exposure sources and estimation of exposure reduction effects by environmental remediation using IEUBK model in lead-contaminated area (2021)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, John Yabe, Kaampwe Muzandu, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 9th Zambia Water Forum and Exhibition
  • Int'l Joint Research
• [Presentation] Evaluation of the ameliorative effect of Spirulina (Arthrospira platensis) supplementation on lead poisoning and obesity in C57BL/6J mice (2021)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, Andrew Kataba, Yared Beyene Yohannes, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 日本アフリカ学会北海道支部オンラインレクチャー
• [Presentation] (Epi) Genetic Effects of δ-Aminolevulinic Acid Dehydratase (ALAD) in Children Exposed to Environmental Lead - a primary study from Kabwe, Zambia (2021)
  • Author(s): Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 48th Annual meeting of Japanese Society of Toxicology
• [Presentation] xenobiotics 代謝と解毒の動物種差 (2021)
  • Author(s): 石塚 真由美 , 中山 翔太 , 池中 良徳
  • Organizer: 48th Annual meeting of Japanese Society of Toxicology
• [Presentation] 鉛中毒マウスの短期作業記憶低下および血中ステロイドの分析 (2021)
  • Author(s): 村上昂暉，中山翔太，一瀬貴大，中田北斗，Yared B. Yohannes，Andrew Kataba，平井杏梨，池中良徳，石塚真由美
  • Organizer: 第164回 日本獣医学会学術集会 日本比較薬理学・毒性学会
• [Presentation] 近年の野生鳥類における鉛中毒 (2021)
  • Author(s): 小笠原浩平、渡辺有希子、安達光、河野晴子、中山翔太、石井千尋、池中良徳、大沼学、石塚真由美、齊藤慶輔
  • Organizer: 第23回環境ホルモン学会研究発表会
• [Presentation] Isotopic Evaluation of the Contribution of Dust from a Mining Site to Heavy Metal Exposure to Wild Lizards (2021)
  • Author(s): Doya Rio, Nakayama M.M. Shouta, Nakata Hokuto, Toyomaki Haruya, Yabe John, Muzandu Kaampwe, Yohannes B. Yared, Kataba Andrew, Kuritani Takeshi, Nakagawa Mitsuhiro, Uchida Yoshitaka, Ikenaka Yoshinori, Ishizuka Mayumi,
  • Organizer: The 9th SaSSOH, 2021
  • Int'l Joint Research
• [Presentation] Accumulation of toxic and trace metals in organs of stray dogs from Kabwe in Zambia (2021)
  • Author(s): Nyein Chan Soe, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Yared B. Yohannes, Kenneth Chawinga, Golden Zyambo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: The 9th SaSSOH, 2021
  • Int'l Joint Research
• [Presentation] Eco-Toxicological Investigation into Lead Exposure to Wild Lizards via Respiratory System (2021)
  • Author(s): Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Haruya Toyomaki, John Yabe, Kaampwe Muzandu, Yared B. Yohannes, Andrew Kataba, Yoshitaka Uchida, Yoshinori Ikenaka, and Mayumi Ishizuka
  • Organizer: The 14th Asian Society of Conservation Medicine
  • Int'l Joint Research
• [Presentation] Wild birds lead poisoning in Honshu area (2021)
  • Author(s): Kohei Ogasawara, Hikaru Adachi, Haruko Kono, Shouta M.M. Nakayama, Naoki Tomita, Chihiro Ishii, Yoshinori Ikenaka, Yasuko Iwami, Yukiko Watanabe, Manabu Onuma, Mayumi Ishizuka, Keisuke Saito
  • Organizer: The 14th Asian Society of Conservation Medicine
  • Int'l Joint Research
• [Presentation] Lead poisoning situation in large raptors in Japan (2021)
  • Author(s): Keisuke Saito, Yukiko Watanabe, Kohei Ogasawara, Chihiro Ishii, Shouta MM Nakayama, Yoshinori Ikenaka, Hikaru Adachi, Haruko Kono, Mayumi Ishizuka
  • Organizer: The 14th Asian Society of Conservation Medicine
  • Int'l Joint Research
• [Presentation] Investigation of Human Exposure Sources and Estimation of Exposure Reduction Effects by Environmental Remediation Using IEUBK Model in Lead-contaminated Area (2021)
  • Author(s): Hokuto Nakata, Shouta M.M. Nakayama, John Yabe, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: SETAC North America 42nd Annual Meeting
  • Int'l Joint Research
• [Presentation] 薬物 /異物代謝と毒性の種差 Species differences in drug metabolism and toxicity (2021)
  • Author(s): 石塚真由美、池中良徳、中山翔太
  • Organizer: 日本薬物動態学会 第36回年会
  • Invited
• [Presentation] Genetic Polymorphism and Promoter Methylation of δ-Aminolevulinic Acid Dehydratase (δ-ALAD) in Children from Kabwe, Zambia (2021)
  • Author(s): Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: 5th International Chemical Hazard Symposium
  • Int'l Joint Research
• [Presentation] The Impact of lead poisoning in Kabwe; a holistic approach and potential mitigation measures (2021)
  • Author(s): Yabe J, Nakayama S, Nakata H, Yohannes YB, Toyomaki H, Muzandu K, Moonga G, Kataba A, Zyambo G, Munyinda NS, Mufune T, Liazambi A, Choongo K, Ikenaka Y, Ishizuka M
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Toxicity assessment of lead in Kabwe residents and its effect of maternal quality of life (2021)
  • Author(s): Nakata H, Nakayama S, Yabe J, Kaampwe M, Tohyama H, Fujita W, Kataba A, Yohannes YB, Toyomaki H, Zyambo G, Munyinda N, Sakala D, Yamsaki S, Nagai N, Yoshida T, Saito T, Choongo K, Ikenaka Y, Ishizuka M
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] EVALAUTION OF LEAD EXPOSURE IN COW MILK FOR RISK ASSESSMENT IN KABWE, ZAMBIA (2021)
  • Author(s): Golden Zyambo, John Yabe, Kaampwe Muzandu, Ethel M’kandawire, Kennedy Choongo, Andrew Kataba, Kenneth Chawinga, Allan Liazambi, Shouta M. M. Nakayama, Hokuto Nakata, Mayumi Ishizuka
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Lead Exposure and its Epi- (Genetic) Effects on Children in Kabwe, Zambia (2021)
  • Author(s): Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Determination of heavy metals and trace elements in organs of stray dogs from Kabwe, Zambia (2021)
  • Author(s): Nyein Chan Soe, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Yared B. Yohannes, Kenneth Chawinga, Golden Zyambo, Kaampwe Muzandu, Andrew Kataba, Patricia Bwalya, Geoffrey Mainda, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Effects of environmentally relevant lead (Pb) levels on the locomotor behaviour and molecular subcellular responses in juvenile zebrafish (Danio rerio) (2021)
  • Author(s): Andrew KATABA, Tarryn L. BOTHA, Shouta M.M. NAKAYAMA, Yared B. YOHANNES, Yoshinori IKENAKA, Victor WEPENER, Mayumi ISHIZUKA
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] How land use patterns affect animal exposure to Pb in Kabwe? (2021)
  • Author(s): Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Haruya Toyomaki, John Yabe, Kaampwe Muzandu, Yared B. Yohannes, Andrew Kataba, Yoshitaka Uchida, Yoshinori Ikenaka, Mayumi Ishizuka
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Efficeint recovery of Pb and Zn from zinc plant leach residues as means of detoxification (2021)
  • Author(s): Marthias Silwamba, Carlito Baltazar Tabelin, Ryota Hashizume, Tomoki Fukushima, Ilhwan Park, Sanghee Jeon, Mayumi Ito, Naoki Hiroyoshi
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Outline of the pilot-scale experiments at the University of Zambia (2021)
  • Author(s): Mufalo, W., Tangviroon, P., Igarashi, T., Ito, M., Sato, T., Chirwa, M., Nyambe, I., Nakata, H., Nakayama, S., Ishizuka, M
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Bio-accessibility of potentially toxic elements from Kabwe mine wastes (2021)
  • Author(s): Mufalo, W., Tangviroon, P., Igarashi, T., Ito, M., Sato, T., Chirwa, M., Nyambe, I., Nakata, H., Nakayama, S., Ishizuka, M
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] Lead-bearing soil dispersion simulations in the Kabwe mine, Zambia (2021)
  • Author(s): Shinsaku Nakamura, Toshifumi Igarashi, Yoshitaka Uchida, Mayumi Ito, Kazuyo Hirose, Tsutomu Sato, Walubita Mufalo, Meki Chirwa, Imasiku Nyambe, Hokuto Nakata, Shouta Nakayama, Mayumi Ishizuka
  • Organizer: International Symposium on Mine Impact on One Health
  • Int'l Joint Research
• [Presentation] ザンビア共和国における鉛汚染問題解決に向けて～多分野融合アプローチによる実践～ (2021)
  • Author(s): 中山翔太
  • Organizer: 令和3年度育志賞研究発表会
• [Presentation] 野生鳥類における鉛中毒問題とその解決に向けて (2021)
  • Author(s): 中山翔太、石井千尋、佐藤裕、一瀬貴大、齊藤慶輔、渡邊有希子、小笠原浩平、安達光、河野晴子、鳥本亮太、小林篤史、木村享史、松川岳久、久保田章乃、横山和仁、池中良徳、石塚真由美
  • Organizer: 日本法獣医学会 第1回学術集会
• [Presentation] 北海道大学 One Health research Center の紹介と中毒診断 (2021)
  • Author(s): 池中良徳、一瀬貴大、中山翔太、石塚真由美
  • Organizer: 日本法獣医学会 第1回学術集会
• [Remarks] 北海道大学大学院獣医学研究院 毒性学教室
  • URL: http://tox.vetmed.hokudai.ac.jp/
• [Funded Workshop] International Symposium on Mine Impact on One Health (2021)