2022 Fiscal Year Final Research Report
Functional analyses of clade-specific virulence genes specified from comparative genome analysis of uncommon genes
| Project/Area Number |
20K21364
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 42:Veterinary medical science, animal science, and related fields
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| Research Institution | Kyoto University (2021-2022)
The University of Tokyo (2020) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
遠矢 真理 順天堂大学, 医学部, 助教 (20804694)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | 豚レンサ球菌 / ゲノム解析 / 非共通領域 / clade特異的病原遺伝子 |
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| Outline of Final Research Achievements |
Streptococcus suis shows extensive diversity from highly virulent to avirulent, however, the real virulence factor has yet been identified. This study aimed to identify the real virulence factor by comparing uncommon genes found on their genome sequences. Complete genome sequences of 2 strains of S. suis of healthy pig origin, 4 strains of S. parsuis, and 3 trains of S. ruminantium, the latter two are the close relatives of S. suis, were determined and compared with those of 62 strains of S. suis and 2 strains of S. ruminantium. Genes for Entner-Doudoroff pathway and hyaluronidase synthesis, which were tandemly clustered, were only found in S. suis and S. ruminantium, whereas some amino acid synthesis genes were only found in S. parasuis.
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| Free Research Field |
獣医細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
Streptococcus suisおよびS. ruiminantiumは，それぞれブタおよび反芻動物の口腔など上部消化器腔内や生殖器粘膜上に正常細菌叢として生息する。一方，S. parasusはブタでは，宿主体内よりも生育環境から多く分離される。これらの３菌種間での ゲノム配列比較によって，エネルギー代謝や宿主組織侵襲性に関連するヒラルロニダーゼ合成の遺伝子の有無や，アミノ酸合成系遺伝子の有無に違いが見られ，それが異なる菌種間での宿主との親和性の違いや自然界における生態を反映しているものと思われ，今後の病原性遺伝子解明や毒力推定に向けた新たな解析方向を示すものとなった。
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