Development of micro total analysis system with an organic light-emitting diode and an organic photodiode for fluorescence detection

2011 Fiscal Year Final Research Report

• Project/Area Number: 21200056
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry; Functional materials/Devices
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: NAKAJIMA Hizuru 首都大学東京, 都市環境科学研究科, 准教授 (10432858)
• Co-Investigator(Kenkyū-buntansha): IMATO Toshihiko 九州大学, 工学研究科, 教授 (50117066)
• Project Period (FY): 2009 – 2011
• Keywords: μTAS / 有機EL / 有機フォトダイオード / CD / ELISA

Research Abstract

A novel micro total analysis system with an organic light-emitting diode and an organic photodiode for fluorescence detection has been developed in order to miniaturize the total size of the analytical system. A compact disk (CD)-type microfluidic device and the solution sending system using centrifugal force was developed. The enzyme-linked immunosorbent assay (ELISA) using the developed system was evaluated. The quantitative values obtained on the developed system were in excellent agreement with that on the conventional ELISA using a 96-well microtiter plate. Since the micro total analysis system developed in this study does not require large size and expensive devices such as laser, microscope, pump and valve, the system would be useful for on-site analysis, such as environmental monitoring, food safety testing and point-of-care testing.

Research Products

• [Journal Article] Performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for IgA (2012)
  • Author(s): Mayo Miyake, Hizuru Nakajima, Akihide Hemmi, Masayuki Yahiro, Chihaya Adachi, Nobuaki Soh, Ryoichi Ishimatsu, Koji Nakano, Katsumi Uchiyama, Toshihiko Imato
  • Journal Title: Talanta,(Available online 10 February 2012) Volume: (in press)
• [Journal Article] An Integrated Enzyme-linked Immunosorbent Assay System with an Organic Light-emitting Diode and a Chargecoupled Device for Fluorescence Detection (2011)
  • Author(s): Hizuru Nakajima, Yukiko Okuma, Kazuhiro Morioka, Mayo Miyake, Akihide Hemmi, Tatsuya Tobita, Masayuki yahiro, Daisuke Yokoyama, Chihaya Adachi, Nobuaki Soh, Koji Nakano, Shuhua Xue, Hulie Zeng, Katsumi Uchiyama, Toshihiko Imato
  • Journal Title: J. Sep. Sci. Volume: 34 Pages: 2906-2912
• [Presentation] An integrated fluorescence detection system using organic light emitting diodes as light source (2010)
  • Author(s): Mayo Miyake, Yukiko Okuma, Akihide Hemmi, Daisuke Yokoyama, Masayuki Yahiro, Chihaya Adachi, Toshihiko Imato, Katsumi Uchiyama, and Hizuru Nakajima
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2010-12-19
• [Presentation] An Integrated Fluorescence Detection System Using Organic Light Emitting Diodes As Light Source (2010)
  • Author(s): Yukiko Okuma, Mayo Miyake, Akihide Hemmi, Toshihiko Imato, Masayuki Yahiro, Chihaya Adachi, Katsumi Uchiyama
  • Organizer: Pittcon2010
  • Place of Presentation: Orland, Florida, USA, (930-62P)
  • Year and Date: 2010-03-01
• [Book] 分析化学用語辞典, 日本分析化学会分析化学用語辞典編集委員会編, (ISBN 978-4-274-21091-4) (2011)
  • Author(s): 中嶋秀, 他56名
  • Total Pages: 461
  • Publisher: オーム社
• [Remarks]
  • URL: http://www.comp.tmu.ac.jp/uchiyama/