2012 Fiscal Year Final Research Report
Molecular characterization of isoquinoline alkaloid biosynthesis and its reconstitution in plant and microbial cells
| Project/Area Number |
21248013
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
SATO Fumihiko 京都大学, 大学院・生命科学研究科, 教授 (10127087)
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| Co-Investigator(Kenkyū-buntansha) |
MINAMI Hiromichi 石川県立大学, 生物資源環境学部, 講師 (90433200)
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| Co-Investigator(Renkei-kenkyūsha) |
IWASA Kinuko 神戸薬科大学, 薬学部, 准教授 (30068340)
IFUKU Kentaro 京都大学, 大学院・生命科学研究科, 助教 (50359783)
SHITAN Nobukazu 神戸薬科大学, 薬学部, 助教 (20547880)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 植物バイオテクノロジー / 薬用植物 / 代謝工学 / 合成生物学 / イソキノリンアルカロイド / 生理活性のスクリーニング |
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| Research Abstract |
Isoquinoline alkaloid biosynthesis was investigated at molecular level to understand their biosynthetic pathways and its regulation. For this purpose, cDNAs of biosynthetic enzymes and transcription factors were isolated and characterized. Genes of biosynthetic enzymes isolated from C. japonicacells were successfully used to reconstitute novel alkaloid fermentation platform with some microbial enzyme genes to produce reticuline from glucose in microbial cells. In addition, bioassay system with Caenorhabditis elegans, to evaluate lipid-accumulation-reducing activity of isoquinoline alkaloids was successfully applied for the screening of new candidate among isoquinoline alkaloids.
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[Journal Article] Bench-top fermentative production of plant benzylisoquinoline alkaloids using a bacterial platform.2012
Author(s)
Nakagawa, A. Minami, H., Kim, J.S., Koyanagi, T., Katayama, T., Sato, F. and Kumagai, H.
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Journal Title
Bioengineered Bugs
Volume: 3
Pages: 49-53
DOI
Peer Reviewed
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[Journal Article] Isoquinoline Alkaloid Biosynthesis is Regulated by a Unique bHLH-type Transcription Factor in Coptis japonica.2011
Author(s)
Yamada, Y., Kokabu, Y., Chaki, K., Yoshimoto, T., Ohgaki, M., Yoshida, S., Kato, N., Koyama, T., and Sato, F.
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Journal Title
Plant Cell Physiol.
Volume: 52
Pages: 1131-1141
DOI
Peer Reviewed
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[Journal Article] A Bacterial Platform for Fermentative Production of Plant Alkaloids.2011
Author(s)
Nakagawa, A., Minami, H., Kim, J.S., Koyanagi, T., Katayama, T., Sato, F. and Kumagai, H.
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Journal Title
DOI
Peer Reviewed
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