2011 Fiscal Year Final Research Report
Induction mechanism of imposex caused by organotin compounds in gastropods : elucidation of involvement of the retinoid X receptor
| Project/Area Number |
21248026
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
HORIGUCHI Toshihiro 独立行政法人国立環境研究所, 環境リスク研究センター, 室長 (30260186)
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| Co-Investigator(Kenkyū-buntansha) |
OTA Yasuhiko 鳥取大学, 農学部, 教授 (60069078)
IGUCHI Taisen 大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (90128588)
MORISHITA Fumihiro 広島大学, 理学研究科, 助教 (20210164)
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| Project Period (FY) |
2009 – 2011
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| Keywords | インポセックス / 有機スズ / レチノイドX受容体 / 神経ペプチド / ステロイド |
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| Research Abstract |
We isolated two isoforms of RXR cDNAs, RXR isoform 1(TcRXR-1) and RXR isoform 2(TcRXR-2), in the rock shell Thais clavigera. The deduced amino acid sequences of TcRXR-1 and TcRXR-2 are highly homologous with those of other gastropods. These TcRXR isoforms displayed 9cRA-dependent activation of transcription in a reporter gene assay using COS-1 cells. The transcriptional activity of TcRXR-2, the product of which has five additional amino acids in the T-box of the C domain, was significantly lower than that of TcRXR-1.Decreases of the transcriptional activity by TcRXR-1 were observed when more than equal amount of TcRXR-2 was existed in a co-transfection assay. Meanwhile, the characteristics of the development of male genitalia(penis and vas deferens) in imposex-exhibiting female T. clavigera, were histologically examined using specimens from a wild population and tributyltin(TBT)-exposed females in the laboratory. A variety of vas deferens morphogenesis patterns were observed in wild female T. clavigera, and the characteristics were summarised. The immature vas deferens at an initial stage, however, was only observed beneath or behind the penis, and no vas deferens was observed close to the vaginal opening(i. e., vulva) of the capsule gland in TBT-exposed females, which was different from the characteristics of vas deferens formation observed in wild females. Taking into consideration both the observed results from wild female specimens and from TBT-exposed females in the laboratory, the vas deferens sequence(VDS) index for T. clavigera was proposed as VDS 1-6.
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[Journal Article] Vas deferens and penis development in the imposex-exhibiting female rock shell2012
Author(s)
Horiguchi, T., Ohta, Y., Urushitani, H., Lee, J. H., Park, J. C., Cho, H. S., Shiraishi, H.
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Journal Title
Thais clavigera, Marine Environmental Research
Volume: VOL. 76
Pages: 71-79
Peer Reviewed
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[Journal Article] Specific accumulation of organotin compounds in tissues of the rock shell2012
Author(s)
Horiguchi, T., Lee, J. H., Park, J. C., Cho, H. S., Shiraishi, H., Morita, M.
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Journal Title
Thais clavigera, Marine Environmental Research
Volume: VOL. 76
Pages: 56-62
Peer Reviewed
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[Journal Article] Cloning and characterization of retinoid X receptor(RXR) isoforms in the rock shell2011
Author(s)
Urushitani, H., Katsu, Y., Ohta, Y., Shiraishi, H., Iguchi, T., Horiguchi, T.
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Journal Title
Thais clavigera, Aquatic Toxicology
Volume: VOL. 103
Pages: 101-111
Peer Reviewed
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[Journal Article] Time course of expression of the retinoid X receptor gene and induction of imposex in the rock shell, Thais clavigera, exposed to triphenyltin chloride2010
Author(s)
Horiguchi, T., Nishikawa, T., Ohta, Y., Shiraishi, H., Morita, M.
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Journal Title
Analytical & Bioanalytical Chemistry
Volume: VOL. 396
Pages: 597-607
Peer Reviewed
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[Presentation] Analysis of the retinoid X receptor(RXR)-mediated mechanism of imposex induced by organotins in gastropods2010
Author(s)
Horiguchi, T., Ohta, Y., Urushitani, H., Iguchi, T., Nishikawa, J., Shiraishi, F., Shimizu, M., Morita, M., Shiraishi, H.
Organizer
Physical and Chemical Impacts on Marine Organisms-For Conservation of Biodiversity and Sustainability-
Place of Presentation
愛知県立大学(愛知県)
Year and Date
2010-10-25
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[Presentation] Recent trends of imposex and organotins content in the rock shell, Thais clavigera from Korean coast2009
Author(s)
Cho, H. S., Park, J. C., Choi, M. K., Choi, H. G., Horiguchi, T.
Organizer
第18回環境化学討論会
Place of Presentation
つくば国際会議場(茨城県)
Year and Date
2009-06-10
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