Mechanism of adrenocortical differentiation by exchange and conversion of enhancers

2011 Fiscal Year Final Research Report

• Project/Area Number: 21249018
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kyushu University
• Principal Investigator: MOROHASHI Ken-ichirou 九州大学, 大学院・医学研究院, 主幹教授 (30183114)
• Project Period (FY): 2009 – 2011
• Keywords: 副腎皮質 / エンハンサー / クロマチン構造

Research Abstract

Ad4BP/SF-1, a member of nuclear receptor superfamily, plays pivotal role in differentiation and function of the adrenal cortex through regulating various target genes. It has been hypothesized that active enhancers should be exchanged or selected through conversion of chromatin structure when Ad4BP/SF-1 functions during the tissue development. In the present study, we performed ChIP-sequence analyses of theDNA recovered with antibodies for Ad4BP/SF-1 and modified histones. As the consequence, it was found that Ad4BP/SF-1 regulates glycolytic gene expression through binding to enhancers localized in and around the genes.

Research Products

• [Journal Article] WNT family genes and their modulation in the ovary-independent and persistent vaginal epithelial cell proliferation and keratinization induced by neonatal diethylstilbesterol exposure in mice (2012)
  • Author(s): T. Nakamura, S. Miyagawa, Y. Katsu, H. Watanabe, T. Mizutani, T. Sato, K. Morohashi, T. Takeuchi, T. Iguchi.
  • Journal Title: Toxicology Volume: 296 Pages: 13-19
  • Peer Reviewed
• [Journal Article] Cbx2, a polycomb group gene, is required for Sry gene expression in mice (2012)
  • Author(s): Y. Katoh-Fukui, K. Miyabayashi, T. Komatsu, A. Owaki, T. Baba, Y. Shima, T. Kidokoro, Y. Kanai, A. Schedl, D. Wilhelm, P. Koopman, Y. Okuno, K. Morohashi.
  • Journal Title: Endocrinology Volume: 153 Pages: 913-924
  • Peer Reviewed
• [Journal Article] Identification of enhancer specific for fetal Leydig cells in Ad4BP/SF-1 gene (2012)
  • Author(s): Y. Shima, K. Miyabayashi, T. Baba, H. Otake, S. Oka, M. Zubair, K. Morohashi.
  • Journal Title: Endocrinology Volume: 153 Pages: 417-425
  • Peer Reviewed
• [Journal Article] SF-1 expression during adrenal development and tumorigenesis. Gardiner JR (2012)
  • Author(s): Y Shima, K. Morohashi, A. Swain
  • Journal Title: Mol. Cell. Endocrinol Volume: 351 Pages: 12-18
  • Peer Reviewed
• [Journal Article] DNA methylation of intronic enhancers directs tissue-specific expression of Steroidogenic Factor 1/Adrenal 4 Binding Protein (2011)
  • Author(s): E. A. Hoivik, T. E. Bjanesoy, O. Mai, S. Okamoto, Y. Minokoshi, Y. Shima, K. Morohashi, U. Boehm, and M. Bakke
  • Journal Title: Endocrinology Volume: 152 Pages: 2100-2112
  • Peer Reviewed
• [Journal Article] The fetal and adult adrenal cortex (2011)
  • Author(s): K. Morohashi and M. Zubair.
  • Journal Title: Mol. Cell. Endocrinol. Volume: 336 1 Pages: 193-197
  • Peer Reviewed
• [Journal Article] Abnormal epithelial cell polarity and migration of Emx2 KO embryonic gonads induced by ectopic EGFR expression (2010)
  • Author(s): M. Kusaka, Y. Katoh-Fukui, H. Ogawa, K. Miyabayashi, T. Baba, N. Sugiyama, Y. Sugimoto, Y. Okuno, R. Kodama, A. Iizuka-Kogo, T. Senda, S. Aizawa, K. Morohashi.
  • Journal Title: Endocrinol Volume: 151 Pages: 5893-5904
  • Peer Reviewed
• [Journal Article] Production and characterization of monoclonal antibodies to germ cells (2010)
  • Author(s): C. Yokoyama, Y. Katoh-Fukui, K. Morohashi, D. Konno, M. Azauma, and T. Tachibana.
  • Journal Title: Hybridoma Volume: 29 Pages: 53-57
  • Peer Reviewed
• [Journal Article] Transcriptional suppression by transient recruitment of ARIP4 to sumoylated nuclear receptor Ad4BP/SF-1 (2009)
  • Author(s): H. Ogawa, T. Komatsu, Y. Hiraoka, and K. Morohashi.
  • Journal Title: Mol. Biol. Cell Volume: 20 Pages: 4235-4245
  • Peer Reviewed
• [Journal Article] Transgenic expression of Ad4BP/SF-1 in fetal adrenal progenitor cells leads to ectopic adrenal formation (2009)
  • Author(s): M. Zubair, S. Oka, K. L. Parker, and K. Morohashi
  • Journal Title: Mol Endocrinol Volume: 23 Pages: 1657-1667
  • Peer Reviewed
• [Journal Article] Generation of rat monoclonal antibodies specific for Ad4BP/SF-1 (2009)
  • Author(s): C. Yokoyama, T. Komatsu, H. Ogawa, K. Morohashi, M. Azuma, and T. Tachibana
  • Journal Title: Hybridoma Volume: 28 Pages: 113-119
• [Journal Article] A critical time window of Sry action in gonadal sex determination (2009)
  • Author(s): R. Hiramatsu, S. Matoba, M. Fujisawa, M. Kanai-Azuma, N. Tsunekawa, M. Kurohmaru, K. Morohashi, D. Wilhelm, P. Koopman, Y. Kanai.
  • Journal Title: Development Volume: 136 Pages: 129-138
  • Peer Reviewed
• [Journal Article] Steroid hormones and the development of reproductive organs
  • Author(s): K. Morohashi, M. Tanaka
  • Journal Title: Sexual Development. in press. Review
  • Peer Reviewed
• [Presentation] 胎仔型ライディッヒ細胞の生理学的機能の解明 (2011)
  • Author(s): 嶋雄一、諸橋憲一郎
  • Organizer: 第84回日本内分泌学会
  • Place of Presentation: 神戸
  • Year and Date: 20110421-23
• [Presentation] 生殖腺におけるステロイドホルモン産生 (2011)
  • Author(s): 諸橋憲一郎
  • Organizer: 第6回日本生殖内分泌学会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-19
• [Presentation] Transcriptomic analysis of fetal Leydig cells (2010)
  • Author(s): Y. Shima, T. Baba, K. Miyabayashi, H. Otake, Y. Kurihara, K. Morohashi
  • Organizer: 第33回日本分子生物学会、第83回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] The Keith Parker Memorial Lecture. Ad4BP/SF-1 as the key factor for steroidogenic cell development (2010)
  • Author(s): K. Morohashi
  • Organizer: 14th Adrenal Cortex Conference
  • Place of Presentation: San Diego, USA
  • Year and Date: 20100916-18
• [Presentation] ステロイド産生細胞の発生 (2010)
  • Author(s): 諸橋憲一郎
  • Organizer: 第28回内分泌代謝サマーセミナー
  • Place of Presentation: 長崎
  • Year and Date: 20100708-10
• [Presentation] Implication of Ad4BP/SF-1 into Adrenal Development (2010)
  • Author(s): K. Morohashi, Y. Shima, K. Miyabayashi, H. Otake, T. Baba, M. Zubair
  • Organizer: 14th International Congress of Endocrinology, ICE2010
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20100326-30
• [Presentation] 生殖腺の性差構築 (2010)
  • Author(s): 諸橋憲一郎
  • Organizer: 第4回日本性差医学・医療学会
  • Place of Presentation: 下関
  • Year and Date: 20100205-06
• [Presentation] Tissue-specific Enhancers of Ad4BP/SF-1 (2010)
  • Author(s): K. Morohashi
  • Organizer: Gene Official Satellite Symposia of ICE2010
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-03-30
• [Presentation] 生殖腺の発生と性分化のメカニズム (2009)
  • Author(s): 諸橋憲一郎、馬場崇、宮林香奈子、大竹博之、嶋雄一
  • Organizer: 第54回日本生殖医学会
  • Place of Presentation: 金沢
  • Year and Date: 20091122-23
• [Presentation] Molecular mechanisms for the functions and structures of tissue specific enhancers (2009)
  • Author(s): Y. Shima, T. Baba, K. Miyabayashi, M. Zubair, K. Morohashi
  • Organizer: Fifth international Symposium on the biology of vertebrate sex determination
  • Place of Presentation: Kona, Hawaii, USA
  • Year and Date: 20090420-24
• [Presentation] Genetic Program of Gonad Differenti (2009)
  • Author(s): 諸橋憲一郎
  • Organizer: 第73回日本循環器学会
  • Place of Presentation: 大阪
  • Year and Date: 20090320-22
• [Presentation] Aug 15-19, Andover, Andover, New Hampshire
  • Author(s): Keynote Lecture, Molecular and Cellular Mechanisms of Testis Differentiation. K. Morohashi
  • Organizer: Gordon Research Conference, 2010 Reproductive Tract Biology
  • Place of Presentation: USA
• [Presentation] Milwaukee
  • Author(s): Lineage of Testicular Leydig cells and Adrenal Cortex. K. Morohashi, Z. Mohamad, T. Baba, K. Miyabayashi, H. Otake, Y. Shima
  • Organizer: 43 rd Annual Meeting of the Society for the Study of Reproduction July 28-Aug
  • Place of Presentation: USA, Invited Speaker
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/seisaseibutu/