Modification for immunological function via Toll-like receptors in macrophages due to physical exercise

2011 Fiscal Year Final Research Report

• Project/Area Number: 21300237
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Kyorin University
• Principal Investigator: OHNO Hideki 杏林大学, 医学部, 教授 (00133819)
• Co-Investigator(Renkei-kenkyūsha): KIZAKI Takako 杏林大学, 医学部, 教授 (00322446) ; SAKURAI Takuya 杏林大学, 医学部, 助教 (20353477) ; OGASAWARA Jyunetsu 杏林大学, 医学部, 助教 (20415110)
• Project Period (FY): 2009 – 2011
• Keywords: 運動 / マクロファージ / Toll様レセプター / 炎症反応 / 感染防御能 / グレリン / 肥満

Research Abstract

Exercise training prevented the infiltration of inflammatory macrophages to adipose tissue and exerted anti-inflammatory effects for obesity-related chronic inflammation. A high fat diet increased Toll-like receptor 2 expression in peritoneal macrophages and enhanced systemic inflammation. On the other hand, exercise training enhanced expression of ghrelin, which exerts potent anti-inflammatory effects in peritoneal macrophages, suggesting that exercise training suppresses a high fat diet-induced inflammation through ghrelin.

Research Products

• [Journal Article] Effect of exercise on HIF-1 and VEGF signaling (2012)
  • Author(s): Ohno, H., Shirato, K., Sakurai, T., Ogasawara, J., Sumitani, Y., Sato, S., Imaizumi, K., Ishida, H. & Kizaki, T
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1 Pages: 5-16
  • Peer Reviewed
• [Journal Article] Copy number variations due to large genomic deletion in x. linked chronic granulomatous disease (2012)
  • Author(s): Arai, T., Oh-Ishi, T., Yamamoto, H., Nunoi, H., Kamizono, J., Uehara, M., Kubota, T., Sakurai, T., Kizaki, T. & Ohno, H.
  • Journal Title: PLoS One Volume: 7 Pages: e27782
  • Peer Reviewed
• [Journal Article] Preventive and improvement effects of exercise training and supplement intake in white adipose tissues on obesity and lifestyle-related diseases (2012)
  • Author(s): Sakurai, T., Ogasawara, J., Kizaki, Takahashi, K., Ishida, H., Miyazaki, H., Saitoh, D., Haga, S., Izawa, T. & Ohno, H.
  • Journal Title: Environ. Health Prev. Med Volume: 17(in press)
  • DOI: DOI:10.1007/s12199-012-0271-0
  • Peer Reviewed
• [Journal Article] The effects of exercise on macrophage function (2012)
  • Author(s): Kizaki, T., Sato, S., Sakurai, T., Ogasawara, J., Imaizumi, K., Izawa, T., Nagasawa, J., Saitoh, D., Haga, S. & Ohno, H.
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1(in press)
  • Peer Reviewed
• [Journal Article] Exercise training and the promotion of neurogenesis and neurite outgrowth in the hippocampus (2012)
  • Author(s): Sakurai, T., Ogasawara, J., Kizaki, T., Ishibashi, Y., Fujiwara, T., Akagawa, K., Izawa, T., Radak, Z. & Ohno, H.
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1(in press)
  • Peer Reviewed
• [Journal Article] Effects of ss_2-agonists and exercise on ss_2-adrenergic receptor signaling in skeletal muscles (2012)
  • Author(s): Sato, S., Shirato, K., Kizaki, T., Ohno, H., Tachiyashiki, K. & Imaizumi, K
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1(in press)
  • Peer Reviewed
• [Journal Article] Effects of exercise on the hexosamine biosynthetic pathway and glycosylation (2012)
  • Author(s): Shirato, K., Kizaki, T., Ohno, H., & Imaizumi, K.
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1(in press)
  • Peer Reviewed
• [Journal Article] Effect of physical exercise on lipolysis in white adipocytes (2012)
  • Author(s): Ogasawara, J., Sakurai, T., Kizaki, T., Takahashi, K., Sumitani, Y., Ishida, H., Izawa, T., Toshinai, K. & Ohno, H.
  • Journal Title: J. Phys. Fit. Sports Med Volume: 1(in press)
  • Peer Reviewed
• [Journal Article] Oxygen consumption and usage during physical exercise : The balance between oxidative stress and ROS-dependent adaptive signaling. Antioxid (2012)
  • Author(s): Radak, Z., Ohno, H. & Atalay, M
  • Journal Title: Redox Signal Volume: 17(in press)
  • Peer Reviewed
• [Journal Article] Erythropoietin, 2, 3 DPG, oxygen transport capacity, and altitude training in adolescent Alpine skiers (2012)
  • Author(s): Son, H. J., Kim, H. J., Kim, J. H., Ohno, H. & Kim, C. K.
  • Journal Title: Aviat. Space Environ. Med Volume: 83 Pages: 50-53
  • Peer Reviewed
• [Journal Article] Induction of mitochondrial uncoupling enhances VEGF_<120> but reduces MCP-1 release in mature 3T3-L1 adipocytes : Possible regulatory mechanism through endogenous ER stress and AMPK-related pathways (2012)
  • Author(s): Miyokawa-Gorin, K., Takahashi, K., Handa, K., Kitahara, A., Sumitani, Y., Katsuta, H., Tanaka, T., Nishida, S., Yoshimoto, K., Ohno, H. & Ishida, H
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 419 Pages: 200205
  • DOI: DOI:10.1016/j.bbrc.2012.01.145
  • Peer Reviewed
• [Journal Article] Influence of mitochondrial DNA 5178 C/A polymorphism on serum cholesterol changes : a short-term follow-up in middle-aged Japanese men (2012)
  • Author(s): Honmyo, R., Kokaze, A., Karita, K., Yoshida, M., Ishikawa, M., & Ohno, H.
  • Journal Title: Environ. Health Prev. Med Volume: 17(in press)
  • DOI: DOI:10.1007/s12199-012-0268-8
  • Peer Reviewed
• [Journal Article] Involvement of leucine zipper transcription factor-like protein 1(Lztfl1) in the attenuation of cognitive impairment by exercise training (2011)
  • Author(s): Sakurai, T., Ogasawara, J., Kizaki, T., Ishibashi, Y., Fujiwara, T., Akagawa, K., Izawa, T., Oh-ishi, S., Haga, S. & Ohno, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 416 Pages: 125-129
  • DOI: DOI:10.1016/j.bbrc.2011.11.008
  • Peer Reviewed
• [Journal Article] Voluntary exercise attenuates obesity-associated inflammation through ghrelin expressed in macrophages (2011)
  • Author(s): Kizaki, T., Maegawa, T., Sakurai, T., Ogasawara, J., Ookawara, T., Oh-ishi, S., Izawa, T., Haga, S. & Ohno, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 413 Pages: 454-459
  • DOI: DOI:10.1016/j.bbrc.2011.08.117
  • Peer Reviewed
• [Journal Article] Augmented bacterial elimination by Kupffer cells after IL-18 pretreatment via IFN-γproduced from NK cells in Burn-injured mice (2011)
  • Author(s): Miyazaki, H., Kinoshita, M., Ono, S., Nakashima, M., Hara, E., Ohno, H., Seki, S. & Saitoh, D.
  • Journal Title: Burns Volume: 37 Pages: 1208-1215
  • DOI: DOI:10.1016/j.burns.2011.04.010
  • Peer Reviewed
• [Journal Article] 富士登山時の自律神経応答と身体の電気伝導率 (2011)
  • Author(s): 長澤純一、内藤祐二郎、杉山康司、笹尾真美、曽我部健、新美敬太、野口いづみ、芳賀脩光、石橋義永、木崎節子、大野秀樹
  • Journal Title: 登山医学 Volume: 31 Pages: 145-152
  • Peer Reviewed
• [Journal Article] 高度が味覚感度へ及ぼす影響-富士山における味覚定性定量検査- (2011)
  • Author(s): 笹尾真美、野口いづみ、新美敬太、曽我部健、井出正道、高野宏二、杉山康司、長澤純一、大野秀樹
  • Journal Title: 登山医学 Volume: 31 Pages: 127-131
  • Peer Reviewed
• [Journal Article] Effect of exercise training on the density of endothelial cells in the white adipose tissue of rats (2011)
  • Author(s): Hatano, D., Ogasawara, J., Endoh, S., Sakurai, T., Nomura, S., Kizaki, T., Ohno, H., Komabayashi, T. & Izawa, T.
  • Journal Title: Scand. J. Med. Sci Volume: 21 Pages: 115-121
  • DOI: DOI:10.1111/j.1600-0838.2010.01176.x
  • Peer Reviewed
• [Journal Article] Comparison of the effect of oligonol, a new lychee fruit-derived low molecular form of polyphenol, and epigallocatechin-3-gallate on lipolysis in rat primary adipocytes (2011)
  • Author(s): Ogasawara, J., Kitadate, K., Nishioka, H., Fujii, H., Sakurai, T., Kizaki, T., Izawa, T., Ishida, H. & Ohno, H.
  • Journal Title: Phytother Res Volume: 25 Pages: 467-471
  • DOI: DOI:10.1002/ptr.3296
  • Peer Reviewed
• [Journal Article] 食細胞の機能と低酸素環境 (2011)
  • Author(s): 大野秀樹、白土健、櫻井拓也、小笠原準悦、石橋義永、芳賀脩光、木崎節子
  • Journal Title: 臨床免疫.アレルギー科 Volume: 55 Pages: 239-246
• [Journal Article] Oligonol, an oligomerized lychee fruit-derived polyphenol, activates the Ras/Raf-1/MEK1/2 cascade independent of the IL-6 signaling pathway in rat primary adipocytes (2010)
  • Author(s): Ogasawara, J., Kitadate, K., Nishioka, H., Fujii, H., Sakurai, T., Kizaki, T., Izawa, T., Ishida, H., Tanno, M. & Ohno, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 402 Pages: 554-559
  • DOI: DOI:10.1016/j.bbrc.2010.10.082
  • Peer Reviewed
• [Journal Article] Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes (2010)
  • Author(s): Ogasawara, J., Nomura, S., Rahman, N., Sakurai, T., Kizaki, T., Izawa, T., Ishida, H., Haga, S. & Ohno, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 400 Pages: 134-139
  • DOI: DOI:10.1016/j.bbrc.2010.08.026
  • Peer Reviewed
• [Journal Article] Effects of exercise training on adipogenesis of stromal-vascular fraction cells in rat epididymal white adipose tissue (2010)
  • Author(s): Sakurai, T., Endo, S., Hatano, D., Ogasawara, J., Kizaki, T., Oh-ishi, S., Izawa, T., Ishida, H. & Ohno, H.
  • Journal Title: Acta Physiol(Oxf) Volume: 200 Pages: 325-338
  • DOI: DOI:10.1111/j.1748-1708.2010.02159.x
  • Peer Reviewed
• [Journal Article] Effect of exercise training on Adipocyte. size-dependent expression of leptin and adiponectin (2010)
  • Author(s): Miyazaki, S., Izawa, T., Ogasawara, J., Sakurai, T., Nomura, S., Kizaki, T., Ohno, H. & Komabayashi, T.
  • Journal Title: Life Sci Volume: 86 Pages: 691-698
  • DOI: DOI:10.1016/j.lfs.2010.03.004
  • Peer Reviewed
• [Journal Article] Exercise intensity and antioxidant ability (2010)
  • Author(s): Nagasawa, J., Kikkawa, K., Takai, T., Sakaguchi, A., Noguchi, I., Kizaki, T. & Ohno, H.
  • Journal Title: Rejuvenation Res Volume: 13 Pages: 172-174
  • DOI: DOI:10.1089/rej.2009.0933
  • Peer Reviewed
• [Journal Article] Exercise plays a preventive role against Alzheimer's disease (2010)
  • Author(s): Radak, Z., Hart, N, Sarga, L., Koltai, E., Atalay, M., Ohno, H. & Boldogh, I.
  • Journal Title: J. Alzheimers Dis Volume: 20 Pages: 777-783
  • DOI: DOI:10.3233/JAD-2010-091531
  • Peer Reviewed
• [Journal Article] 低圧.低酸素環境が引き起こす酸化ストレスとオリゴノールの抗酸化効果 (2010)
  • Author(s): 長澤純一、杉山康塚、内丸仁、笹尾真美、高野宏二、野口いづみ、鈴木康弘、北舘健太郎、芳賀脩光、前川剛輝、櫻井拓也、小笠原準悦、木崎節子、大野秀樹
  • Journal Title: 登山医学 Volume: 30 Pages: 118-124
  • Peer Reviewed
• [Journal Article] 中国青海省の玉珠峰(6178 m)短期登頂の医学的検証-日中韓三国交流学生登山における検討- (2010)
  • Author(s): 野口いづみ、小坂健一郎、笹尾真美、前川剛輝、高野宏二、大野秀樹
  • Journal Title: 登山医学 Volume: 30 Pages: 125-130
  • Peer Reviewed
• [Journal Article] 登山の魅力と効能:登山は少子化を救えるか (2010)
  • Author(s): 大野秀樹
  • Journal Title: 地域総合研究 Volume: 11 Pages: 249-266
• [Journal Article] Oligomerized grape seed polyphenols attenuate inflammatory changes due to antioxidative properties in co-culture of adipocytes and macrophages (2009)
  • Author(s): Sakurai, T., Kitadate, K., Nishioka, K., Fujii, H., Kizaki, T., Kondoh, Y., Izawa, T., Ishida, H., Radak, Z. & Ohno, H.
  • Journal Title: J Nutr. Biochem Volume: 21 Pages: 47-54
  • Peer Reviewed
• [Journal Article] Exercise training decreases expression of inflammation-related adipokines through reduction of oxidative stress in rat white adipose tissue (2009)
  • Author(s): Sakurai, T., Izawa, T., Kizaki, T., Ogasawara, J., Shirato, K., Imaizumi, K., Takahashi, K., Ishida, H. & Ohno, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 379 Pages: 605-609
  • DOI: DOI:10.1016/j.bbrc.2008.12.127
  • Peer Reviewed
• [Journal Article] Oligonol, a new lychee fruit-derived low-molecular form of polyphenol, enhances lipolysis in primary rat adipocytes through activation of the ERK1/2 pathway (2009)
  • Author(s): Ogasawara, J., Kitadate, K., Nishioka, H., Fujii, H., Sakurai, T., Kizaki, T., Izawa, T., Ishida, H. & Ohno, H.
  • Journal Title: Phytother. Res Volume: 23 Pages: 1626-1633
  • DOI: DOI:10.1002/ptr.2846
  • Peer Reviewed
• [Journal Article] Hypoxia-inducible factor-1αsuppresses the expression of macrophage scavenger receptor 1 (2009)
  • Author(s): Shirato, K., Kizaki, T., Sakurai, T., Ogasawara, J., Ishibashi, Y., Iijima, T., Okada, C., Noguch, I., Imaizumi, K., Taniguchi, N. & Ohno, H.
  • Journal Title: Pflugers Arch Volume: 459 Pages: 93-103
  • DOI: DOI:10.1007/s00424-009-0702-y
  • Peer Reviewed
• [Journal Article] Swimming training prevents induction of suppressor macrophages in mice during acute exposure to cold (2009)
  • Author(s): Ohno, H., Haga, S., Takemasa, T., Sakurai, T., Ogasawara, J., Shirato, K., Ishibashi, Y., Imaizumi, K. & Kizaki, T.
  • Journal Title: Adv. Exerc. Sports Physiol Volume: 15 Pages: 1-7
  • Peer Reviewed
• [Journal Article] 新規低分子ポリフェノールOligonolの抗酸化作用 (2009)
  • Author(s): 櫻井拓也、北舘健太郎、西岡浩、藤井創、久島達也、安部茂、木崎節子、小笠原準悦、白土健、石橋義永、井澤鉄也、今泉和彦、鈴木健二、中野法彦、大野秀樹
  • Journal Title: FOOD FUNCTION Volume: 5 Pages: 2-7
• [Journal Article] 低酸素誘導因子(HIF)-1αはマクロファージスカベンジャー受容体1の発現を抑制する (2009)
  • Author(s): 大野秀樹、白土健、前川剛輝、櫻井拓也、小笠原準悦、石橋義永、木崎節子、野口いづみ、長澤純一、荻原理江、貫田宗男、鈴木健二、大石修司、今泉和彦、芳賀脩光
  • Journal Title: 登山医学 Volume: 29 Pages: 203-212
  • Peer Reviewed
• [Presentation] Oligonolは神経細胞において小胞体ストレス減弱作用をもつ (2012)
  • Author(s): 櫻井拓也, ほか
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都
  • Year and Date: 20120324-26
• [Book] 機能性食品素材と運動療法-生活習慣病予防と運動機能維持向上を目指して- (2012)
  • Author(s): 大野秀樹, ほか
  • Publisher: シーエムシー出版(印刷中)
• [Patent(Industrial Property Rights)] 遺伝子導入促進剤及びそれを含む遺伝導入用組成物 (2010)
  • Inventor(s): 人見嘉哲、大野秀樹
  • Industrial Property Rights Holder: 人見嘉哲、大野秀樹
  • Industrial Property Number: 特許、特許第4499035号
  • Acquisition Date: 2010-04-23