Establishment of in vitro gut inflammatory model and search and mechanism of food factor possessing anti-inflammatory activit y

2011 Fiscal Year Final Research Report

• Project/Area Number: 21300276
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kobe University
• Principal Investigator: MIZUNO Masashi 神戸大学, 大学院・農学研究科, 教授 (00212233)
• Co-Investigator(Kenkyū-buntansha): NISHITANI Yosuke 神戸大学, 自然科学系先端融合研究環重点研究部, 助教 (80457093)
• Project Period (FY): 2009 – 2011
• Keywords: 健康と食生活

Research Abstract

A system for assessing the anti-inflammatory effect of food factors has been developed by establishing a co-culture system with intestinal epithelial Caco-2 cells(apical side) and macrophage RAW264. 7 cells(basolateral side) stimulated with lipopolysaccharide. Curcumin and luteolin as polyphenols, fucoidan and lentinan as polysaccharide shows the anti-inflammatory activity in this system. Particularly, it was demonstrated that fucoidan indicated its activity by producing hydrogen peroxide from Caco-2 cells.

Research Products

• [Journal Article] β-Glucan from Lentinus edodes inhibits nitric oxide and tumor necrosis factor-□production and phosphorylation of mitogen-activated protein kinases in lipopolysaccharide-stimulated murine RAW 264. 7 (2012)
  • Author(s): Xu, X. J., Yasuda, M., Tsuruta, S., Mizuno, M., and Ashida, H.
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 871-878
  • Peer Reviewed
• [Journal Article] TLR ligands of Lactobacillus sakei LK-117 isolated from seed mash for brewing sake are potent inducers of IL-12 (2011)
  • Author(s): Masuda, Y., Takahashi, T., Yoshida, K., Nishitani, Y., Mizuno, M. and Mizoguchi, H.
  • Journal Title: J. Biosci. Bioeng. Volume: 112 Pages: 363-368
  • Peer Reviewed
• [Journal Article] Oral treatment with extract of Agaricus blazei Murill enhanced Th1 response through intestinal epithelial cells and suppressed OVA-sensitized allergy in mice (2011)
  • Author(s): Bouike, G., Nishitani, Y., Shiomi, H., Yoshida, M., Azuma, T., Hashimoto, T., Kanazawa, K. and Mizuno, M.
  • Journal Title: Evid. Based Complement. Alternat Volume: Volume Pages: 11
  • DOI: doi:10.1155/2011/532180
  • Peer Reviewed
• [Journal Article] Anti-inflammatory activities of Lactococcus lactis subsp. cremoris FC in in vitro and in vivo gut inflammation (2010)
  • Author(s): Nishitani, Y. and Mizuno, M.
  • Journal Title: Biosci. Microflora Volume: 29 Pages: 169-178
  • Peer Reviewed
• [Journal Article] Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga (2010)
  • Author(s): Nagasato, C., Inoue, A., Mizuno, M., Kanazawa, K., Ojima, T., Okuda, K. and Motomura, T.
  • Journal Title: Silvetia babingtonii Volume: 232 Pages: 287-298
  • Peer Reviewed
• [Journal Article] Lipoxin A4 reduces lipopolysaccharide-induced inflammation in macrophages and intestinal epithelial cells through inhibition of NF-kappaB activation (2010)
  • Author(s): Kure, I., Nishiumi, S., Nishitani, Y., Tanoue, T., Ishida, T., Mizuno, M., Fujita, T., Kutsumi, H., Arita, M., Azuma, T. and Yoshida, M.
  • Journal Title: J. Pharmacol. Exp. Ther. Volume: 332 Pages: 541-548
  • Peer Reviewed
• [Journal Article] Different suppressive effects of fucoidan and lentinan on IL-8 mRNA expression in in vitro gut inflammation (2009)
  • Author(s): Mizuno, M., Nishitani, Y., Hashimoto, T. and Kanazawa, K.
  • Journal Title: Biosci. Biotech. Biochem. Volume: 73 Pages: 2324-2325
  • Peer Reviewed
• [Journal Article] Lactococcus lactis subsp. cremoris FC alleviates symptoms of colitis induced by dextran sulfate sodium in mice (2009)
  • Author(s): Nishitani, Y., Tanoue, T., Yamada, K., Ishida, T., Yoshida, M., Azuma, T. and Mizuno, M.
  • Journal Title: Int. Immunopharmacol. Volume: 9 Pages: 1444-1451
  • Peer Reviewed
• [Journal Article] The distribution and accumulation of fucoxanthin and its metabolites after oral-administration in mice (2009)
  • Author(s): Hashimoto, T., Ozaki, Y., Taminato, M., Das, S. K., Mizuno, M., Yoshimura, K., Maoka, T. and Kanazawa, K.
  • Journal Title: Br. J. Nutr Volume: 102 Pages: 242-248
  • Peer Reviewed
• [Journal Article] Anti-inflammatory effect of Bifidobacterium longum on macrophage-like THP-1 cells via epithelial cell Caco-2 (2009)
  • Author(s): Takizawa, R., Nishitani, Y., Mizuno, M. and Osawa, R.
  • Journal Title: Biosci. Microflora Volume: 28 Pages: 45-48
  • Peer Reviewed
• [Journal Article] Quantitation and localization of fucoidan in Laminaria japonica using a novel antibody (2009)
  • Author(s): Mizuno, M., Nishitani, Y., Tanoue, T., Matoba, Y., Ojima, T., Hashimoto, T. and Kanazawa, K.
  • Journal Title: Biosci. Biotech. Biochem Volume: 73 Pages: 335-338
  • Peer Reviewed
• [Presentation] レンチナンの腸管炎症抑制機構:小腸上皮様Caco-2細胞のTNFR1の局在性に及ぼす影響について (2012)
  • Author(s): 水野雅史
  • Organizer: 日本農芸化学会平成24年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-24
• [Presentation] Gut anti-inflammator activity of lentinan : influence on IL-8 and TNFR1 expression in intestinal epithelial-like Caco-2 cell (2011)
  • Author(s): Masashi Mizuno
  • Organizer: 16th European Carbohydrate Symposiu
  • Place of Presentation: Sorrento, Ital
  • Year and Date: 2011-07-04
• [Presentation] In vitro腸炎モデルのおけるレンチナンの炎症抑制機構の検討 (2011)
  • Author(s): 水野雅史
  • Organizer: 日本農芸化学会平成23年度大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-27
• [Book] プロバイオティクスの炎症性腸疾患抑制作用、免疫機能性食品の基礎と応用 (2010)
  • Author(s): 西谷洋輔、水野雅史
  • Total Pages: 134-144
  • Publisher: シーエムシー出版
• [Book] βグルカン高含有食品素材の免疫賦活活性、βグルカンの基礎と応用-感染、抗がん、ならびに機能性食品へのβグルカンの関与- (2010)
  • Author(s): 水野雅史
  • Total Pages: 127-132
  • Publisher: シーエムシー出版
• [Book] In vitro and in vivo immunnomodulatory and anti-allergic effects of Agaricus blazei Murill, In Dietary Components and Immune Functio (2010)
  • Author(s): Mizuno, M.
  • Total Pages: 387-394
  • Publisher: Humana Pres
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/ans-glyco-chain/