Analysis of flower coloration and control mediated by multi-enzyme complexes

2011 Fiscal Year Final Research Report

• Project/Area Number: 21310135
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Tohoku University
• Principal Investigator: NAKAYAMA Toru 東北大学, 大学院・工学研究科, 教授 (80268523)
• Project Period (FY): 2009 – 2011
• Keywords: メタボロン / フラボノイド / 酵素間相互作用 / キンギョソウ / フラボン / アント シアニン / オーロン

Research Abstract

I have clarified that, in snapdragon petal cell, enzymes that are related to biosynthesis of flavones and anthocyanins form multi-enzyme complexes, which are spatially and functionally separated from enzymes related to aurone biosynthesis.

Research Products

• [Journal Article] Transcription analyses of GmICHG, a gene coding for a β-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr (2013)
  • Author(s): Yoo, D.C., Hara, T., Fujita, N., Waki, T., Noguchi, A., Takahashi, S., Nakayama, T.
  • Journal Title: Plant Sci Volume: 208 Pages: 10-19
  • URL: http://dx.doi.org/10.1016/j.plantsci.2013.03.006
  • Peer Reviewed
• [Journal Article] Purification, gene cloning, and biochemical characterization of a β-glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. KB0549 (2013)
  • Author(s): Nair, A., Kuwahara, A. Nagase, A. Yamaguchi, H. Yamazaki, T., Hosoya, M., Omura, A., Kiyomoto, K., Yamaguchi, M., Shimoyama, T., Takahashi, S., and Nakayama, T.
  • Journal Title: PLOS ONE Volume: 8 Pages: e60538
  • DOI: doi:10.1371/journal.pone.0060538
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysisof UDP-glucuronic acid :flavonol-3-O-glucuronosyltransferase (2013)
  • Author(s): Mizohata, E., Okuda, T., Hatanaka, S., Nakayama, T., Horikawa, M., Nakayama, T., Ono, E., and Inoue, T.
  • Journal Title: (VvGT5) from the grapevine Vitis vinifera. Acta Cryst Volume: F69 Pages: 65-68
  • DOI: doi:10.1107/S1744309112045095
  • Peer Reviewed
• [Journal Article] メタボロン…植物二次代謝工学におけるインパクト (2012)
  • Author(s): 中山亨*・兪東燦・高橋征司
  • Journal Title: 生物工学 Volume: 90 Pages: 576-581
• [Journal Article] Synthesis of3,5,3′,4′-tetrahydroxy- trans-stilbene-4′-O-β-D-glucopyranoside by glucosyltransferases from Phytolacca Americana Nat (2012)
  • Author(s): Iwakiri, T., Imai, H., Hamada H., Nakayama, T. and Ozaki, S.
  • Journal Title: Prod. Commun Volume: 8 Pages: 119-120
  • Peer Reviewed
• [Journal Article] Purification, characterization, and primary structure of a novel N-acyl-D-amino acid amidohydrolase from Microbacterium natoriense TNJL143-2 (2012)
  • Author(s): Liu, J., Asano, Y., Ikoma, K., Yamashita, S., Hirose, Y., Shimoyama, T., Takahashi, S., Nakayama, T. and Nishino, T.
  • Journal Title: J. Biosci. Bioengin Volume: 114 Pages: 391-397
  • DOI: doi:10.1016/j.jbiosc.2012.05.015
  • Peer Reviewed
• [Journal Article] Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevines (2010)
  • Author(s): Ono, E., Homma, Y., Horikawa, M., Kunikane-Doi, S., Imai, H., Takahashi, S., Kawai, Y., Ishiguro, M., Fukui, Y., Nakayama,T
  • Journal Title: Plant Cell Volume: 22 Pages: 2856-2871
  • URL: www.plantcell.org/cgi/doi/10.1105/tpc.110.074625
  • Peer Reviewed
• [Journal Article] New role of flavin as a general acid-base catalyst with no dedox function in type 2 isopentenyl-diphosphate isomerase (2009)
  • Author(s): Unno, H., Yamashita, S., Ikeda, Y., Sekiguchi, S., Yoshida, N., Yoshimura, T., Kusunoki,M., Nakayama, T. Nishino, T., Hemmi, H.
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 9160-9167
  • DOI: doi:10.1074/jbc.M808438200
  • Peer Reviewed
• [Journal Article] Local differentiation of sugar donor speciocity of flavonoid glycosyltransferase in Lamiales (2009)
  • Author(s): Noguchi, A., Horikawa, M., Fukui, Y., Fukuchi-Mizutani, M., Iuchi-Okada, A., Ishiguro, M., Kiso, Y., Nakayama, T., Ono, E.
  • Journal Title: Plant Cell Volume: 21 Pages: 1556-1572
  • URL: www.plantcell.org/cgi/doi/10.1105/tpc.108.063826
  • Peer Reviewed
• [Presentation] Purification, gene cloning, and biochemical characterization of a β-Glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. Strain KB0549 (2012)
  • Author(s): Nakayama, T
  • Organizer: 6th Japan-Finland Biotechnology Symposium 2012
  • Place of Presentation: 東北学院大学土 樋キャンパス(仙台市)
  • Year and Date: 20120604-20120608
• [Presentation] ブドウのフラボノール3位グリコシルトランスフェラーゼにおける糖供与体特異性の進化 (2011)
  • Author(s): 中山亨
  • Organizer: 日本応用酵素協会第37回研究発表会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-14
• [Presentation] ブドウの生理活性フラボノイド配糖体の生合成に関わる糖転移酵素(GT)の機能進化 (2011)
  • Author(s): 中山亨
  • Organizer: 第426回ビタミンB研究協議会
  • Place of Presentation: 大津(ピアザ淡海)
  • Year and Date: 2011-11-05
• [Presentation] キンギョソウにおける花色発現の代謝戦略 (2011)
  • Author(s): 中山亨
  • Organizer: 日本生化学会シンポジウム「植物の高次機能を司る機能性低分子化合物」
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] 多様な生物に見られるユニークな代謝と酵素:機能・進化解析と応用への展望 (2011)
  • Author(s): 中山亨
  • Organizer: ダイズイソフラボン生合成酵素群にみられるユニークな代謝と酵素日本農芸化学会シンポジウム
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2011-03-25
• [Presentation] 酵素・タンパク質のユニークな活性調節機構と機能発現の新展開 (2010)
  • Author(s): 中山亨
  • Organizer: 植物グリコシルトランスフェラーゼの機能進化.日本農芸化学会シンポジウム
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-03-30
• [Presentation] Comparative biochemistry of plant glucuronosyltransferases. Italy-Japan Sympojium (2009)
  • Author(s): Nakayama, T
  • Organizer: New Trends in Enzyme Science and Technology
  • Place of Presentation: イタリアナポリ
  • Year and Date: 20091026-20091029
• [Presentation] 植物におけるグルクロノシル化反応の比較生化学第2回博多シンポジウム (2009)
  • Author(s): 中山亨
  • Organizer: 内外環境と生物応答
  • Place of Presentation: 博多
  • Year and Date: 20091009-20091010
• [Presentation] Comparative studies on biochemistry and phylogenetics of three glucuronosyltransferases involved in anthocyanins andrelated flavonoids (2009)
  • Author(s): Nakayama, T
  • Organizer: 5th International Workshop on Anthocyanins,2009 in Japan
  • Place of Presentation: 名古屋
  • Year and Date: 20090915-20090918
• [Presentation] バイオで開く21世紀の科学 (2009)
  • Author(s): 中山亨
  • Organizer: 黄色い花もいかが?～酵素ハンティングと花色改変～日本化学会東北支部地区講演会
  • Place of Presentation: 山形大学(山形市)
  • Year and Date: 2009-11-26