2011 Fiscal Year Final Research Report
Capture of diatomic molecules by supramolecular heme protein models and application to development to medicinal chemistry
| Project/Area Number |
21350097
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Doshisha University |
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Principal Investigator |
KANO Koji 同志社大学, 理工学部, 教授 (60038031)
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| Co-Investigator(Kenkyū-buntansha) |
NEGI Shigeru 同志社女子大学, 薬学部, 助手 (50378866)
KAWAGUCHI Akira 東海大学, 医学部, 准教授 (30195052)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 超分子化学 / ナノバイオ / ヘムタンパク質 |
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| Research Abstract |
HemoCD, which is composed of a water-soluble iron porphyrin(FeTPPS) and a per-methylated β-cyclodextrin dimer(Py3CD), was attached to poly(acrylic acid) s or gold nano-particles to inhibit glomerular filtration of hemoCD. These modified hemoCDs, which were regarded as the hemoglobin(Hb) and myoglobin(Mb) models, were not excluded in the urine of a rat, suggesting that the modified hemoCDs stay in the blood for a long time. We also tried to apply the present supramolecular system to medicinal chemistry. At first, the removal of endogenous carbon monoxide of a rat was examined by infusing hemoCD solution into the femoral vein. Injected hemoCD was rapidly excreted in the urine in the form of a carbon monoxide adduct, indicating that hemoCD can be used as an antidote of CO poisoning. Meanwhile, hemoCD was unfavorable for the cyanide poisoning. Then we prepared a supramolecule, Im3CD/Fe(III) TPPS complex, which strongly captured the cyanide anion in a rat's body. Since this cyanide remover did not interact with bovine proteins, the Im3CD/Fe(III) TPPS complex was assumed to be better antidote than hydroxocobalamin.
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