2011 Fiscal Year Final Research Report
Analysis of affinity maturation process of antibodies using a novel FDC line and its application.
| Project/Area Number |
21360405
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
OMORI Hitoshi 岡山大学, 大学院・自然科学研究科, 教授 (70116440)
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| Co-Investigator(Renkei-kenkyūsha) |
MAGARI Masaki 岡山大学, 大学院・自然科学研究科, 助教
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| Project Period (FY) |
2009 – 2011
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| Keywords | モノクローナル抗体 / リンパ球 / 突然変異 / アポトーシス / 生体防御 / 胚中心 / 抗体医薬 / 濾胞樹状細胞 |
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| Research Abstract |
Using a novel murine follicular dendritic cell line, FL-Y, I analyzed affinity maturation process of antibodies focusing on B cell selection in germinal centers(GCs). I found that FL-Y cells induced B cell death in the presence of a helper T cell cytokine, IL-21. The B cell death was shown to be dependent on PGE2 produced from FL-Y cells, The IL-21/PGE2-induced B cell death implicates a new negative selection mechanism of B cells in GCs.
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