2011 Fiscal Year Final Research Report
Selfish DNases at a diverging point to survival, death and evolution
| Project/Area Number |
21370001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOBAYASHI Ichizo 東京大学, 大学院・新領域創成科学研究科, 教授 (30126057)
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| Co-Investigator(Renkei-kenkyūsha) |
HANDA Naofumi 東京大学, 大学院・新領域創成科学研究科, 特任助教 (00396855)
TAKAHASHI Noriko 東京大学, 大学院・新領域創成科学研究科, 特任助教 (70466829)
MIKIHIKO Kawai 東京大学, 大学院・新領域創成科学研究科, 特任研究員 (80451904)
FURUTA Yoshikazu 東京大学, 大学院・新領域創成科学研究科, 特任助教 (40613667)
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| Research Collaborator |
UCHIYAMA Ikuo 基礎生物学研究所, ゲノム情報研究室, 助教 (90243089)
JANUSZ Bujnicki IIMCB, Poland
KOJI Yahara 学振, 特別研究員(PD)
MASAKI Fukuyo 学振, 特別研究員(PD)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ゲノム構築 / ゲノム再編 / ゲノム維持 / ゲノム進化 |
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| Research Abstract |
Our experimental analyses and genome comparisons revealed how DNAases distinguishing self and non-self genome acted on genomes and drove their evolution.1. We revealed role of antisense RNA in their gene regulation and life cycle.2. We showed several restriction enzymes cleave a DNA replication fork.3. With restriction enzyme attacking the chromosome, we demonstrated success of suicidal defense strategy against infection.4. Genome comparison of H. pylori revealed novel mechanisms of genome rearrangements and suggested that restriction modification systems frequently switch their recognition sequence.
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