2012 Fiscal Year Final Research Report
Elucidation of structure-function of unique bacterial flagellar stator complex from Bacillus spp.
| Project/Area Number |
21370074
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Toyo University |
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Principal Investigator |
ITO Masahiro 東洋大学, 生命科学部, 教授 (80297738)
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| Co-Investigator(Kenkyū-buntansha) |
TERAHARA Naoya 東洋大学, バイオナノエレクトロニクス研究センター, 研究助手 (40554738)
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| Co-Investigator(Renkei-kenkyūsha) |
IMADA Katsumi 大阪大学, 大学院・理学研究科, 教授 (40346143)
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| Project Period (FY) |
2009 – 2012
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| Keywords | べん毛 / バチルス / 膜タンパク質 / 構造解析 / 固定子 |
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| Research Abstract |
At first, we identified that the flagellar motor of B. subtilis was a hybrid motor and it rotated by using both ion motive forces, H+ and Na+, by a single molecular mechanism. Next, the critical charged amino acid residues between the second and third transmembrane cytoplasmic loop region in MotA and MotP subunits of B. subtilis were identified by using site-directed mutagenesis. Moreover, we identified a novel type flagellar motor from alkaliphilic Bacillus alcalophilus which stimulates swimming speed under elevated K+ or Rb+ concentrations. This is the first report that describes aflagellar motor that can use both K+ and Rb+ as coupling ions.
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[Journal Article] Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4.2011
Author(s)
Janto B, Ahmed A, Ito M, Liu J, Hicks DB, Pagni S, Fackelmayer OJ, Smith T A, Earl J, Elbourne LD, Hassan K, Pau lsen IT, Kolsto AB, Tourasse NJ, Ehrl ich GD, Boissy R, Ivey DM, Li G, Xue Y, Ma Y, Hu FZ, Krulwich TA.
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Journal Title
Environment al Microbiology
Volume: 13(12)
Pages: 3289?3309
Peer Reviewed
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