2011 Fiscal Year Final Research Report
Studies on microbial aromatic L-amino acid decarboxylase
| Project/Area Number |
21380062
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
KUMAGAI Hidehiko 石川県立大学, 生物資源環境学部, 特任教授 (70027192)
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| Co-Investigator(Kenkyū-buntansha) |
KATAYAMA Takane 石川県立大学, 生物資源工学研究所, 准教授 (70346104)
KOYANAGI Takashi 石川県立大学, 食品科学科, 助教 (20535041)
MATSUI Hiroshi (40336730)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 芳香族アミン / 芳香族アミノ酸脱炭酸酵素 / ビタミンB_6酵素 / 発酵生産 / 発酵食品 / 菌叢解析 / 次世代シークエンス法 |
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| Research Abstract |
Aromatic amino acid decarboxylase is a key enzyme for synthesis of various biogenic amines and their derivatives. In this study, we isolated the genes encoding the enzyme from various bacteria, and carried out enzymatic analyses for their products. We also succeeded in constructing a bacterial platform for synthesis of the plant alkaloid using a recombinant E. coli strain. In addition, fermented food microbiota was investigated by using pyrosequencing technique to evaluate the existing ratio of lactic acid bacteria with abilities to produce biogenic amines.
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[Journal Article] Changes in microbiota population during fermentation of Narezushi, as revealed by pyrosequencing analysis2012
Author(s)
清原正志, 小柳喬, 松井裕, 山本恵子, 武春美, 勝山陽子, 辻篤志, 宮前博人, 近藤哲史, 中村静夫, 片山高嶺, 熊谷英彦
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 76巻
Pages: 48-52
Peer Reviewed
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