2011 Fiscal Year Final Research Report
Elucidation of mobile loop functions in food-related enzymes
| Project/Area Number |
21380064
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MIKAMI Bunzo 京都大学, 大学院・農学研究科, 教授 (40135611)
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| Project Period (FY) |
2009 – 2011
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| Keywords | タンパク質工学 / 応用構造生物学 / X線結晶構造解析 / 酵素反応機構 |
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| Research Abstract |
The functions of proteins are usually expressed by conformational changes in loop regions. We have investigated the mobile loops in food-related enzymes that are used in food industry. The mobile loops in the active sites of.-amylase and alginate lyase that are important for incorporation of substrate and release of product are analyzed by X-ray crystal analyses of their mutant enzymes. In order to reveal the enzyme mechanism of protein-glutaminase, a mutant of pro protein-glutaminase(A47Q) was prepared. It was found that the mutated Gln47 formed Michaelis complex and S-acyl covalent intermediate with a catalytic cysteine residue.
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