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2011 Fiscal Year Final Research Report

Studies on physiological roles of ER-60 by tissue-specific gene targeting analysis

Research Project

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Project/Area Number 21380081
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionKyoto University

Principal Investigator

URADE Reiko  京都大学, 大学院・農学研究科, 教授 (90167289)

Co-Investigator(Kenkyū-buntansha) URADE Yoshihiro  大阪バイオサイエンス研究所, 研究部長 (10201360)
Co-Investigator(Renkei-kenkyūsha) KASHIWAGI Kahori  大阪バイオサイエンス研究所, 研究員 (10372666)
LAZARUS Michae  大阪バイオサイエンス研究所, 研究員 (80469650)
Project Period (FY) 2009 – 2011
KeywordsER-60 / 小胞体 / 分子シャペロン / ノックアウトマウス / 小胞体ストレス / アミロイドβ / X線結晶構造解析
Research Abstract

In this project, we found that the folding efficiency of some membrane glycoproteins was impaired by liver-specific knockout of ER-60. It was demonstrated that ER stress might be easily induced in ER-60 knockout mice by long feeding of high-fat diet. We attempted to confirm the protective effects of ER-60 against toxicity of amyloid beta peptides by brain-specific ER-60 knockout. In addition, we succeeded in X-ray structural analysis of a complex with the bb' fragment of ER-60 andβ-amyloid peptide.

  • Research Products

    (17 results)

All 2012 2011 2010 2009

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (7 results) Book (2 results)

  • [Journal Article] Accumulation ofβ-conglycinin in soybean cotyledon through the formation of disulfide bonds betweenα'-andα-subunits2012

    • Author(s)
      Wadahama H, Iwasaki K, Matsusaki M, Nishizawa K, Ishimoto M, Arisaka F, Takagi K, Urade R.
    • Journal Title

      Plant Physiol

      Volume: 158 Pages: 1395-1405

    • DOI

      DOI:10.1104/pp.11.189621

    • Peer Reviewed
  • [Journal Article] Acute administration of fluoxetine normalizes rapid eye movement sleep abnormality but not depressive behaviors in olfactory bulbectomized rats2012

    • Author(s)
      Wang YQ, Tu ZC, Xu XY, Li R, Qu WM, Urade Y, Huang ZL.
    • Journal Title

      J. Neurochem

      Volume: 120 Pages: 314-324

    • DOI

      DOI:10.1111/j.1471-4159.2011.07558.x

    • Peer Reviewed
  • [Journal Article] High-Quality Protein Crystal Growth of Mouse Lipocalin-Type Prostaglandin D Synthase in Microgravity2011

    • Author(s)
      Inaka K, Takahashi S, Aritake K, Tsurumura T, Furubayashi N, Yan B, Hirota E, Sano S, Sato M, Kobayashi T, Yoshimura Y, Tanaka H, Urade Y.
    • Journal Title

      Cryst. Growth Des

      Volume: 11 Pages: 2107-2111

    • DOI

      DOI:10.1021/cg101370v

    • Peer Reviewed
  • [Journal Article] Arousal effect of caffeine depends on adenosine A2A receptors in the shell of the nucleus accumbens2011

    • Author(s)
      Lazarus M, Shen HY, Cherasse Y, Qu W M, Huang Z L, Bass C E, Winsky-Sommerer R, Semba K, Fredholm B B, Boison D, Hayaishi O, Urade Y, Chen JF.
    • Journal Title

      J. Neurosci

      Volume: 31 Pages: 10067-10075

    • DOI

      DOI:10.1523/JNEUROSCI.6730-10.2011

    • Peer Reviewed
  • [Journal Article] A novel and comprehensive mouse model of human non-alcoholic steatohepatitis with the full range of dysmetabolic and histological abnormalities induced by gold thioglucose and a high-fat diet2010

    • Author(s)
      Ogasawara M, Ogasawara M, Hirose A, Ono M, Aritake K, Nozaki Y, Takahashi M, Okamoto N, Sakamoto S, Iwasaki S, Asanuma T, Taniguchi T, Urade Y, Onishi S, Saibara T, Oben JA
    • Journal Title

      Liver Int

      Volume: 31 Pages: 542-551

    • DOI

      DOI:10.1111/j.1478-3231.2010.02443.x.

    • Peer Reviewed
  • [Journal Article] Relationship between endogenous protein disulfide isomerase family proteins and gluten in macro polymer2010

    • Author(s)
      Koh A, Nishimura K, Urade R
    • Journal Title

      J. Agric. Food Chem

      Volume: 58 Pages: 12970-12975

    • DOI

      DOI:10.1021/jf103347p

    • Peer Reviewed
  • [Journal Article] Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs2009

    • Author(s)
      Iwasaki K, Kamauchi S, Wadahama H, Ishimoto M, Kawada T and Urade R
    • Journal Title

      FEBS J.

      Volume: 276 Pages: 4130-4141

    • DOI

      DOI:10.1111/j.1742-4658.2009.07123.x

    • Peer Reviewed
  • [Journal Article] Changes in lipid metabolism by soy beta-conglycinin-derived peptides2009

    • Author(s)
      Mochizuki, Y., Maebuchi, M., Kohno, M., Hirotsuka M, Wadahama H, Moriyama T, Kawada T and Urade R
    • Journal Title

      J. Agric. Food Chem

      Volume: 57 Pages: 1473-1480

    • DOI

      DOI:10.1021/jf8031793

    • Peer Reviewed
  • [Presentation] ダイズ子葉細胞におけるジスルフィド結合を介したβ-コングリシニン複合体の形成2012

    • Author(s)
      和田濱裕之、岩崎健佑、石本政男、西澤けいと、裏出令子
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-24
  • [Presentation] 脳におけるER-60の発現量とレプチン作用との関係2012

    • Author(s)
      裏出令子、和田濱裕之、有竹浩介、永田奈々恵、裏出良博
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-23
  • [Presentation] 小胞体分子シャペロンER-60の基質結合ドメインとアミロイドβペプチド複合体の結晶構造解析2012

    • Author(s)
      伊中浩二、古林直樹、裏出良博、魚留信子、鶴村俊治、和田濱裕之、裏出令子
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2012-03-23
  • [Presentation] ER-60ノックアウトの肝細胞小胞体機能への影響2011

    • Author(s)
      高木悠希, 有竹浩介, 大石陽, 鈴木比佐子, 和田濱裕之, 河田照雄, 裏出良博, 裏出令子
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2011-03-05
  • [Presentation] 肝臓特異的ER-60/ERp57ノックアウトの小胞体機能への影響2010

    • Author(s)
      高木悠希, 有竹浩介, 大石陽, 鈴木比佐子, 和田濱裕之, 河田照雄, 裏出良博, 裏出令子
    • Organizer
      日本生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-10
  • [Presentation] レプチンによる小胞体分子シャペロンの発現調節2009

    • Author(s)
      和田濱裕之、有竹浩介、裏出良博、河田照雄、裏出令子
    • Organizer
      日本生化学会大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-21
  • [Presentation] Thiol oxidoreductases involved in soybean storage protein biogenesis2009

    • Author(s)
      Urade R
    • Organizer
      34th FEBS Congress
    • Place of Presentation
      Prague Congress Centre Czech Republic
    • Year and Date
      2009-07-05
  • [Book] Elsevier, Fortification of bread with soy proteins to normalize serum cholesterol and triacylglycerol levels in Flour and Breads and their Fortification in Health and Disease Prevention2011

    • Author(s)
      Urade R
    • Total Pages
      417-427
    • Publisher
      Academic Press
  • [Book] Sophisticated modes of sugar recognition by the intracellular lectins involved in quality control of glycoproteins in Glycobiology Research Trends2009

    • Author(s)
      Kamiya Y, Kamiya D, Urade R, Suzuki T and Kato K
    • Total Pages
      27-40
    • Publisher
      Nova Science Publishers, Inc.

URL: 

Published: 2013-07-31  

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