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2011 Fiscal Year Final Research Report

Regulation of APOBEC3 gene expression and its transcript splicing

Research Project

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Project/Area Number 21390143
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionKinki University

Principal Investigator

MIYAZAWA Masaaki  近畿大学, 医学部, 教授 (60167757)

Co-Investigator(Kenkyū-buntansha) KAWAHARA Sachiyo  近畿大学, 医学部, 講師 (60297629)
HAKATA Yoshiyuki  近畿大学, 医学部, 助教 (30344500)
TAKAMURA Shiki  近畿大学, 医学部, 助教 (90528564)
Project Period (FY) 2009 – 2011
Keywords個体 / 感染抵抗性 / 宿主因子 / 遺伝子発現
Research Abstract

Polymorphisms at the mouse APOBEC3(mA3) gene locus have been associated with different susceptibilities to infection with mouse leukemia viruses. In virus-resistant C57BL/6(B6) mice, mA3 transcripts and protein are more abundant than those in susceptible BALB/c mice, and these strains of mice also express mA3 transcripts with different splicing patterns. B6 mice express predominantly the exon 5-deficient transcript, which confer more efficient translation than the full-length transcript. By employing in vitro splicing assays using genomic DNA clones, we identified two critical determinants that regulate exon 5 inclusion into mA3 transcripts : the number of TCCT repeats upstream of exon 5 and the newly identified single nucleotide polymorphism within exon 5 located 12 bases upstream of the exon 5/intron 5 boundary.

  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (13 results) Book (1 results) Remarks (1 results)

  • [Journal Article] 生理的に機能するレトロウイルス複製制限因子APOBEC3の分子進化2012

    • Author(s)
      宮澤正顯
    • Journal Title

      ウイルス

      Volume: 62(印刷中)

    • Peer Reviewed
  • [Journal Article] Two genetic determinants acquired late in Mus evolution regulate the inclusion of exon 5, which alters mouse APOBEC3 translation efficiency2012

    • Author(s)
      Li, J., Y. Hakata, E. Takeda, Q. Liu, Y. Iwatani, C. A. Kozak, M. Miyazawa
    • Journal Title

      PLoS Pathogens

      Volume: 8 Pages: e1002478

    • DOI

      DOI:10.1371/journal.ppat.1002478

    • Peer Reviewed
  • [Journal Article] An evolutionary analysis of RAC2 identifies haplotypes associated with human autoimmune diseases2011

    • Author(s)
      Sironi, M., F. R. Guerini, C. Agliardi, M. Biasin, R. Cagliani, M. Fumagalli, D. Caputo, A. Cassinotti, S. Ardizzone, M. Zanzottera, E. Bolognesi, S. Riva, Y. Kanari, M. Miyazawa, M. Clerici
    • Journal Title

      Mol. Biol. Evol

      Volume: 28 Pages: 3319-3329

    • DOI

      DOI:10.1093/molbev/msr164

    • Peer Reviewed
  • [Journal Article] Natural killer cells recognize Friend retrovirusinfected erythroid progenitor cells through NKG2D-RAE-1 interactions in vivo2011

    • Author(s)
      Ogawa, T., S. Tsuji-Kawahara, T. Yuasa, S. Kinoshita, T. Chikaishi, S. Takamura, H. Matsumura, T. Seya, T. Saga, M. Miyazawa
    • Journal Title

      J. Virol

      Volume: 85 Pages: 5423-5435

    • DOI

      DOI:10.1128/JVI.02146-10

    • Peer Reviewed
  • [Journal Article] Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in Rgs16 knockdown mice2011

    • Author(s)
      Hayasaka, N., K. Aoki, S. Kinoshita, S. Yamaguchi, J. K. Wakefield, S. Tsuji-Kawahara, K. Horikawa, H. Ikegami, S. Wakana, T. Murakami, R. Ramabhadran, M. Miyazawa, S. Shibata
    • Journal Title

      PLoS ONE

      Volume: 6 Pages: e17655

    • DOI

      DOI:10.1371/journal.pone.0017655

    • Peer Reviewed
  • [Journal Article] Protection of macaques with diverse MHC genotypes against a heterologous SIV by vaccination with a deglycosylated live-attenuated SIV2010

    • Author(s)
      Sugimoto C., S. Watanabe, T. Naruse, E. Kajiwara, T. Shiino, N. Umano, K. Ueda, H. Sato, S. Ohgimoto, V. Hirsch, F. Villinger, A. A. Ansari, A. Kimura, M. Miyazawa, Y. Suzuki, N. Yamamoto, Y. Nagai, K. Mori
    • Journal Title

      PLoS One

      Volume: 5 Pages: e11678

    • DOI

      DOI:10.1371/journal.pone.0011678

    • Peer Reviewed
  • [Journal Article] Diversity of MHC class I genes in Burmese-origin rhesus macaques2010

    • Author(s)
      Naruse, T. K., Z. Chen, R. Yanagida, T. Yamashita, Y. Saito, K. Mori, H. Akari, Y. Yasutomi, M. Miyazawa, T. Matano, A. Kimura
    • Journal Title

      Immunogenetics

      Volume: 62 Pages: 601-611

    • DOI

      DOI:10.1007/s00251-010-0462-z

    • Peer Reviewed
  • [Journal Article] Response to comment on "Premature terminal exhaustion of Friend virus-specific effector CD8^+T cells by rapid induction of multiple inhibitory receptors."2010

    • Author(s)
      Takamura, S., M. Miyazawa
    • Journal Title

      J. Immunol

      Volume: 185 Pages: 1349-1350

    • DOI

      DOI:jimmunol.1090059

    • Peer Reviewed
  • [Journal Article] Persistence of viremia and production of neutralizing antibodies differentially regulated by polymorphic APOBEC3 and BAFF-R loci in Friend virus-infected mice2010

    • Author(s)
      Tsuji-Kawahara, S., T. Chikaishi, E. Takeda, M. Kato, S. Kinoshita, E. Kajiwara, S. Takamura, M. Miyazawa
    • Journal Title

      J. Virol

      Volume: 84 Pages: 6082-6095

    • DOI

      DOI:10.1128/JVI.02516-09

    • Peer Reviewed
  • [Journal Article] Premature terminal exhaustion of Friend virus-specific effector CD8^+T cells by rapid induction of multiple inhibitory receptors2010

    • Author(s)
      Takamura, S., S. Tsuji-Kawahara, H. Yagita, H. Akiba, M. Sakamoto, T. Chikaishi, M. Kato, M. Miyazawa
    • Journal Title

      J. Immunol

      Volume: 184 Pages: 4696-4707

    • DOI

      DOI:10.4049/jimmunol.0903478

    • Peer Reviewed
  • [Journal Article] Replies : The 'immunologic advantages' of HIV-exposed seronegative individuals2009

    • Author(s)
      Miyazawa, M., M. Clerici
    • Journal Title

      AIDS

      Volume: 23 Pages: 1612

    • DOI

      DOI:10.1097/QAD.0b013e32832d74ca

    • Peer Reviewed
  • [Journal Article] The "immunologic advantage" of HIV-exposed seronegative individuals2009

    • Author(s)
      Miyazawa M., L. Lopalco, F. Mazzotta, S. Lo Caputo, F. Veas, M. Clerici
    • Journal Title

      AIDS

      Volume: 23 Pages: 161-175

    • DOI

      DOI:10.1097/QAD.0b013e3283196a80

    • Peer Reviewed
  • [Presentation] Resistance to HIV infection and AIDS progression2012

    • Author(s)
      Miyazawa, M
    • Organizer
      NEKKEN Research Conference : HIV Patients Care and Research in Southeast Asia
    • Place of Presentation
      Nagasaki, Japan
    • Year and Date
      20120322-23
  • [Presentation] Production of virus-neutralizing antibodies and protection against lethal retroviral infection in AID-deficient mice2011

    • Author(s)
      Kato, M., et al.
    • Organizer
      第40回日本免疫学会学術集会
    • Place of Presentation
      千葉
    • Year and Date
      20111127-29
  • [Presentation] Infection of thymus with murine retrovirus induces virus-specific central tolerance that prevents dynamic differentiation of functional memory CD8^+T cells2011

    • Author(s)
      Takamura, S., et al.
    • Organizer
      第40回日本免疫学会学術集会
    • Place of Presentation
      千葉
    • Year and Date
      20111127-29
  • [Presentation] Functional consequences of mouse APOBEC3 gene polymorphisms and multiple genetic factors that influence the production of virus-neutralizing antibodies in Friend virus-infected mice2011

    • Author(s)
      Miyazawa, M., et al.
    • Organizer
      The 23rd Workshop on Retrovirla Pathogenesis
    • Place of Presentation
      Montpellier, Farance
    • Year and Date
      20111102-05
  • [Presentation] Host immune responses determine integration of either F-MuLV alone or F-MuLV plus SFFV in Friend virus leukemogenesis2011

    • Author(s)
      Miyazawa, M., et al.
    • Organizer
      The 23rd Workshop on Retrovirla Pathogenesis
    • Place of Presentation
      Montpellier, Farance
    • Year and Date
      20111102-05
  • [Presentation] A hole in the T-cell repertoire induced after retroviral infection of immunocompetent adult mice2011

    • Author(s)
      Miyazawa, M., et al.
    • Organizer
      Frontiers of Retrovirology 2011
    • Place of Presentation
      Amsterdam, The Netherlands
    • Year and Date
      20111003-05
  • [Presentation] HIV感染抵抗性の分子機構: Rac2とAPOBEC3(シンポジウム)2010

    • Author(s)
      宮澤正顯, 他
    • Organizer
      第24回日本エイズ学会学術集会・総会
    • Place of Presentation
      東京
    • Year and Date
      20101124-26
  • [Presentation] レトロウイルス遺伝子発現と糸球体病変:拡大するヒトレトロウイルスの世界2010

    • Author(s)
      宮澤正顯
    • Organizer
      第45回日本小児腎臓病学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      20100702-03
  • [Presentation] High-level Rac2 expression associated with novel intron polymorphisms restricts HIV-1 replication in exposed seronegative individuals2010

    • Author(s)
      Kanari, Y., et al.
    • Organizer
      NEKKEN Research Conference "Ten years achievements of the Lampang HIV cohort in Northen Thailand."
    • Place of Presentation
      Nagasaki, Japan
    • Year and Date
      2010-10-27
  • [Presentation] Genetic factors that confer resistance to HIV-1 acquisition in HIV-1-exposed but seronegative individuals in Italy and Thailand2009

    • Author(s)
      Miyazawa, M., et al.
    • Organizer
      4th Nagasaki Symposium on Tropical and Emerging Infectious Diseases
    • Place of Presentation
      Nagasaki
    • Year and Date
      20091126-28
  • [Presentation] Mouse APOBEC3 affects the production of virus-neutralizing antibodies by restricting early retroviral replication, not by altering the B-cell repertoire2009

    • Author(s)
      Miyazawa, M., et al.
    • Organizer
      Frontiers of Retrovirology 2011
    • Place of Presentation
      Montpellier, France
    • Year and Date
      20090921-23
  • [Presentation] Mechanisms of immune evasion by Friend virus : T-cell exhaustion, B-cell hyperactivation, and their genetic control2009

    • Author(s)
      Miyazawa, M., et al.
    • Organizer
      21st International Workshop on Retroviral Pathogenesis
    • Place of Presentation
      Castelnuovo Garfagnana, Lucca, Italy
    • Year and Date
      20090913-17
  • [Presentation] New metabolic markers derived from gamma and deltaretrovirus envelope glycoproteins2009

    • Author(s)
      Sitbon, M., et al.
    • Organizer
      21st International Workshop on Retroviral Pathogenesis
    • Place of Presentation
      Castelnuovo Garfagnana, Lucca, Italy
    • Year and Date
      20090913-17
  • [Book] 解明病理学:病気のメカニズムを解く2009

    • Author(s)
      宮澤正顯, 青笹克之
    • Total Pages
      84-129
    • Publisher
      医歯薬出版
  • [Remarks]

    • URL

      http://www.med.kindai.ac.jp/immuno/konnano.htm

URL: 

Published: 2013-07-31  

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