ヒト肝細胞キメラマウスと超高密度アレイによる肝臓の網羅的トランスクリプトーム解析

2011 Fiscal Year Annual Research Report

• Project/Area Number: 21390229
• Research Institution: Hiroshima University
• Principal Investigator: 茶山 一彰 広島大学, 大学院・医歯薬学総合研究科, 教授 (00211376)
• Keywords: 肝臓学 / 転写 / 発現抑制 / 発現亢進

Research Abstract

近年、ゲノムの98%をしめるnoncoding regionにもmRNA、tRNA、rRNA以外のRNAが大量にコードされていることが発見され、しかも、それらのかなりの部分は何らかの機能を有していることが明らかにされた。その発現状態は、細胞の状況により様々に変化することが想定される。本研究では、B型肝炎ウイルス、C型肝炎ウイルスの感染、あるいはインターフェロン投与により引き起こされる種々の転写の変化を測定し、ウイルス感染に応答して起こる細胞内の変化を理解し、ウイルス感染に対する治療のターゲットとなる分子を明らかにすることを目的とて研究を行った。B型肝炎ウイルスクローン、あるいはC型肝炎ウイルスクローンを使用して感染マウスを作製した。B型肝炎ウイルスでは、Huh7細胞にクローン化DNAをtransfectionし、産生されたウイルスをマウスに静脈内投与した。C型肝炎ウイルスの場合はクローンDNAからin vitro transcriptionにより転写されたウイルスRNAを肝臓に直接注入した。得られた肝炎ウイルスクローン感染マウスあるいは、コントロールの非感染マウスを用いて以下の4群において実験を行っている。1)PBS投与群、2)ウイルス非感染IFN投与群(6時間後sacrifice)3)肝炎ウイルス感染、IFN非投与群、4)肝炎ウイルス感染、IFN投与群(投与6時間後sacrifice)。各群n=5で解析を行った。これらの実験の結果、B型肝炎ウイルス感染では細胞周期のコントロールに関連する遺伝子が、C型肝炎ウイルス感染では炎症に関連するサイトカインなどの遺伝子が増加することが示された。さらに、C型では代謝に関連する遺伝子の転写が抑制されることが明らかになった。量ウイルスに感染することによりIFNのシグナルに関しても影響があることが明らかにし、それぞれ論文として報告した。

Research Products

• [Journal Article] Impact of pegylated interferon therapy on outcomes of patients with hepatitis C virus-related hepatocellular carcinoma after curative hepatic resection (2012)
  • Author(s): Tanimoto Y, Tashiro H, Aikata H, Amano H, Oshita A, Kobayashi T, Kuroda S, Tazawa H, Takahashi S, Itamoto T, Chayama K, Ohdan H
  • Journal Title: Ann Surg Oncol Volume: 19 Pages: 418-425
  • DOI: 10.1245/s10434-011-1866-1
  • Peer Reviewed
• [Journal Article] Severe necroinflammatory reaction caused by natural killer by Fas/FasL interaction and dendritic cells in human hepatocyte chimeric mouse (2012)
  • Author(s): Okazaki A, Hiraga N, Imamura M, Nelson Hayes C, Tsuge M, Takahashi S, Aikata H, Abe H, Miki D, Ochi H, Tateno C, Yoshizato K, Ohdan H, Chayama K
  • Journal Title: Hepatology Volume: (in press)
  • DOI: 10.1002/hep.25651
  • Peer Reviewed
• [Journal Article] IL28B polymorphism is associated with fatty change in the liver of chronic hepatitis C patients (2012)
  • Author(s): Ohnishi M, Tsuge M, Kohno T, Zhang Y, Abe H, Hyogo H, Kimura Y, Miki D, Hiraga N, Imamura M, Takahashi S, Ochi H, Hayes CN, Tanaka S, Arihiro K, Chayama K
  • Journal Title: J Gastroenterol Volume: (in press)
  • DOI: 10.1007/s00535-012-0550-y
  • Peer Reviewed
• [Journal Article] Serum PAI-1 is a novel predictor for response to pegylated interferon-al pha-2b plus ribavirin therapy in chronic hepatitis C virus infection (2012)
  • Author(s): Miki D, Ohishi W, Ochi H, Hayes CN, Abe H, Tsuge M, Imamura M, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: J Viral Hepat Volume: 19 Pages: e126-133
  • DOI: 10.1111/j.1365-2893.2011.01516.x
  • Peer Reviewed
• [Journal Article] Hepatocellular carcinoma : towards personalized medicine (2012)
  • Author(s): Miki D, Ochi H, Nelson Hayes C, Aikata H, Chayama K
  • Journal Title: Cancer Sci Volume: (in press)
  • DOI: 10.1111/j.1349-7006.2012.02242.x
  • Peer Reviewed
• [Journal Article] Combination of hepatitis B viral antigens and DNA for prediction of relapse after discontinuation of nucleos(t)ide analogs in patients with chronic hepatitis B (2012)
  • Author(s): Matsumoto A, Tanaka E, Suzuki Y, Kobayashi M, Tanaka Y, Shinkai N, Hige S, Yatsuhashi H, Nagaoka S, Chayama K, Tsuge M, Yokosuka O, Imazeki F, Nishiguchi S, Saito M, Fujiwara K, Torii N, Hiramatsu N, Karino Y, Kumada H
  • Journal Title: Hepatol Res Volume: 42 Pages: 139-149
  • DOI: 10.1111/j.1872-034X.2011.00910.x
  • Peer Reviewed
• [Journal Article] Efficacy and safety of telaprevir, a new protease inhibitor, for difficult-to-treat patients with genotype 1 chronic hepatitis C (2012)
  • Author(s): Hayashi N, Okanoue T, Tsubouchi H, Toyota J, Chayama K, Kumada H
  • Journal Title: J Viral Hepat Volume: 19 Pages: e134-142
  • DOI: 10.1111/j.1365-2893.2011.01528.x
  • Peer Reviewed
• [Journal Article] Prevalence and associated metabolic factors of nonalcoholic fatty liver disease in the general population from 2009 to 2010 in Japan : a multicenter large retrospective study (2012)
  • Author(s): Eguchi Y, Hyogo H, Ono M, Mizuta T, Ono N, Fujimoto K, Chayama K, Saibara T, Jsg N
  • Journal Title: J Gastroenterol Volume: (in press)
  • DOI: 10.1007/s00535-012-0533-z
  • Peer Reviewed
• [Journal Article] Validation of the FIB4 index in a Japanese nonalcoholic fatty liver disease population (2012)
  • Author(s): Sumida Y, Yoneda M, Hyogo H, Itoh Y, Ono M, Fujii H, Eguchi Y, Suzuki Y, Aoki N, Kanemasa K, Fujita K, Chayama K, Saibara T, Kawada N, Fujimoto K, Kohgo Y, Yoshikawa T, Okanoue T, Jsg-Nafld JS
  • Journal Title: BMC Gastroenterol Volume: (Epub)
  • DOI: 10.1186/1471-230X-12-2
  • Peer Reviewed
• [Journal Article] Identification of the Niemann-Pick C1-like 1 cholesterol absorption receptor as a new hepatitis C virus entry factor (2012)
  • Author(s): Sainz B, Jr., Barretto N, Martin DN, Hiraga N, Imamura M, Hussain S, Marsh KA, Yu X, Chayama K, Alrefai WA, Uprichard SL
  • Journal Title: Nat Med Volume: 18 Pages: 281-285
  • DOI: 10.1038/nm.2581
  • Peer Reviewed
• [Journal Article] Toward the establishment of a prediction system for the personalized treatment of chronic hepatitis C (2012)
  • Author(s): Ochi H, Hayes CN, Abe H, Hayashida Y, Uchiyama T, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: J Infect Dis Volume: 205 Pages: 204-210
  • DOI: 10.1093/infdis/jir726
  • Peer Reviewed
• [Journal Article] Telaprevir with peginterferon and ribavirin for treatment-naive patients chronically infected with HCV of genotype 1 in Japan (2012)
  • Author(s): Kumada H, Toyota J, Okanoue T, Chayama K, Tsubouchi H, Hayashi N
  • Journal Title: J Hepatol Volume: 56 Pages: 78-84
  • DOI: 10.1016/j.jhep.2011.07.016
  • Peer Reviewed
• [Journal Article] Atorvastatin improves disease activity of nonalcoholic steatohepatitis partly through its tumour necrosis factor-alpha-lowering property (2012)
  • Author(s): Hyogo H, Yamagishi SI, Maeda S, Kimura Y, Ishitobi T, Chayama K
  • Journal Title: Dig Liver Dis Volume: (in press)
  • DOI: 10.1016/j.dld.2011.12.013
  • Peer Reviewed
• [Journal Article] Dual therapy with the nonstructural Protein 5A Inhibitor, BMS-790052, and the Nonstructural Protein 3 Protease Inhibitor, BMS-650032, in Hepatitis C Virus Genotype 1b-Infected Null Responders (2012)
  • Author(s): Chayama K, Takahashi S, Toyota J, Karino Y, Ikeda K, Ishikawa H, Watanabe H, McPhee F, Hughes E, Kumada H
  • Journal Title: Hepatology Volume: 55 Pages: 742-8
  • DOI: 10.1002/hep.24724
  • Peer Reviewed
• [Journal Article] Amino Acid Substitution in HCV Core/NS5A Region and Genetic Variati on Near IL28B Gene Affect Treatment Efficacy to Interferon plus Ribavirin Combination Therapy (2012)
  • Author(s): Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Chayama K, Nakamura Y, Kumada H
  • Journal Title: Intervirology Volume: 55 Pages: 231-41
  • DOI: 10.1159/000328327
  • Peer Reviewed
• [Journal Article] Will there be an HCV meeting in 2020? Summary of the 17th international meeting on hepatitis C virus and related viruses (2011)
  • Author(s): Wakita T, Suzuki T, Evans MJ, Shimotohno K, Chayama K, Matsuura Y, Hijikata M, Moriishi K, Seya T, Enomoto N, Koike K, Kato N, Kanto T, Hotta H
  • Journal Title: Gastroenterology Volume: 141 Pages: e1-5
  • DOI: doi:10.1053/j.gastro.2011.05.027.
  • Peer Reviewed
• [Journal Article] Effects of hepatitis B virus infection on the interferon response in immunodeficient human hepatocyte chimeric mice (2011)
  • Author(s): Tsuge M, Takahashi S, Hiraga N, Fujimoto Y, Zhang Y, Mitsui F, Abe H, Kawaoka T, Imamura M, Ochi H, Hayes CN, Chayama K
  • Journal Title: J Infect Dis Volume: 204 Pages: 224-228
  • DOI: doi:10.1093/infdis/jir247.
  • Peer Reviewed
• [Journal Article] In vivo adaptation of hepatitis C virus in chimpanzees for efficient virus production and evasion of apoptosis (2011)
  • Author(s): Saeed M, Shiina M, Date T, Akazawa D, Watanabe N, Murayama A, Suzuki T, Watanabe H, Hiraga N, Imamura M, Chayama K, Choi Y, Krawczynski K, Liang TJ, Wakita T, Kato T
  • Journal Title: Hepatology Volume: 54 Pages: 425-433
  • DOI: doi:10.1002/hep.24399.
  • Peer Reviewed
• [Journal Article] Impact of radiation and hepatitis virus infection on risk of hepatocellular carcinoma (2011)
  • Author(s): Ohishi W, Fujiwara S, Cologne JB, Suzuki G, Akahoshi M, Nishi N, TsugeM, Chayama K
  • Journal Title: Hepatology Volume: 53 Pages: 1237-1245
  • DOI: doi:10.1002/hep.24207.
  • Peer Reviewed
• [Journal Article] A genome-wide association study of chronic hepatitis B identified novel risk locus in a Japanese population (2011)
  • Author(s): Mbarek H, Ochi H, Urabe Y, Kumar V, Kubo M, Hosono N, Takahashi A, Kamatani Y, Miki D, Abe H, Tsunoda T, Kamatani N, Chayama K, Nakamura Y, Matsuda K
  • Journal Title: Hum Mol Genet Volume: 20 Pages: 3884-3892
  • DOI: doi:10.1093/hmg/ddr301.
  • Peer Reviewed
• [Journal Article] A randomized phase II trial of intra-arterial chemotherapy using SM-11355 (Miriplatin) for hepatocellular carcinoma (2011)
  • Author(s): Okusaka T, Kasugai H, Ishii H, Kudo M, Sata M, Tanaka K, Shioyama Y, Chayama K, Kumada H, Yoshikawa M, Seki T, Saito H, Hayashi N, Shiratori K, Okita K, Sakaida I, Honda M, Kusumoto Y, Tsutsumi T, Sakata K
  • Journal Title: Invest New Drugs (Epub 21 Dec.) Volume: (E-pub)
  • DOI: 10.1007/s10637-011-9776-4
  • Peer Reviewed
• [Journal Article] Treatment of chronic hepatitis B with nuoleos(t)ide analogues (2011)
  • Author(s): Ohishi W, Chayama K
  • Journal Title: Hepatol Ees Volume: 42 Pages: 219-25
  • DOI: 10.1111/j.1872-034X.2011.00921.x
  • Peer Reviewed
• [Journal Article] Recent trend of clinical features in patients with hepatocellular carcinoma (2011)
  • Author(s): Nagaoki Y, Hyogo H, Aikata H, Tanaka M, Naeshiro N, Nakahara T, Honda Y, Miyaki D, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Takahashi S, Chayama K
  • Journal Title: Hepatol Res (Epub 13 Dec.) Volume: (Epub)
  • DOI: 10.1111/j.1872-034X.2011.00929.x
  • Peer Reviewed
• [Journal Article] Platelet count for predicting fibrosis in nonalcoholic fatty liver disease (2011)
  • Author(s): Yoneda M, Fujii H, Sumida Y, Hyogo H, Itoh Y, Ono M, Eguchi Y, Suzuki Y, Aoki N, Kanemasa K, Imajo K, Chayama K, Saibara T, Kawada N, Fujimoto K, Kohgo Y, Yoshikawa T, Okanoue T, Japan Study Group of Nonal coholic Fatty Liver D
  • Journal Title: J Gastroenterol Volume: 46 Pages: 1300-1306
  • DOI: 10.1007/s00535-011-0436-4
  • Peer Reviewed
• [Journal Article] Clinical outcome of esophageal varices after hepatic arterial infusion chemotlierapy for advanced hepatocellular carcinoma with major portal vein tumor thrombus (2011)
  • Author(s): Kodama H, Aikata H, Murakami E, Miyaki D, Nagaoki Y, Hashimoto Y, Azakami T, Katamura Y, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Takahashi S, Ishikawa M, Kakizawa H, Awai K, Kenio M, Nagata Y, Chayama K
  • Journal Title: Hepatol Res Volume: 41 Pages: 1046-1056
  • DOI: 10.1111/j.1872-034X.2011.00857.x
  • Peer Reviewed
• [Journal Article] Eradication of hepatitis C virus genotype 1 after liver transplantation by interferon therapy before surgery : Report of three patients with analysis of interleukin-28 polymorphism, hepatitis C virus core region and interferon-sensitivity determining region (2011)
  • Author(s): Kawaoka T, Aikata H, Miyaki D, Murakami E, Azakami T, Takaki S, Nagaoki Y, Hashimoto Y, Katamura Y, Hiramatsu A, Waki K, Hiraga N, Miki D, TsugeM, Imamura M, Kawakami Y, Takahashi S, Ochi H, Tashiro H, Ohdan H, Chayama K
  • Journal Title: Hepatol Res Volume: 41 Pages: 1126-1131
  • DOI: 10.1111/j.1872-034X.2011.00853.x
  • Peer Reviewed
• [Journal Article] A huge metastatic liver tumor from leiomyosarcoma of the inferior vena cava : report of a case (2011)
  • Author(s): Hashimoto M, Kobayashi T, Tashiro H, Amano H, Oshita A, Tanimoto Y, Kuroda S, Tazawa H, Aikata H, Chayama K, Fujii M, Arihiro K, Ohdan H
  • Journal Title: Surg Today (Epub 30 Nov.) Volume: (Epub)
  • DOI: 10.1007/s00595-011-0070-z
  • Peer Reviewed
• [Journal Article] Lymphocyte subset characterization associated with persistent hepatitis C virus infection and subsequent progression of liver fibrosis (2011)
  • Author(s): Yoshida K, Ohishi W, Nakashima E, Fujiwara S, Akahoshi M, Kasagi F, Chayama K, Hakoda M, Kyoizumi S, Nakachi K, Hayashi T, Kusunoki Y
  • Journal Title: Hum Immunol Volume: 72 Pages: 821-826
  • DOI: 10.1016/j.humimm.2011.05.029
  • Peer Reviewed
• [Journal Article] Prevention of hepatitis B virus reactivation in immunosuppressive therapy or chemotherapy (2011)
  • Author(s): Ohishi W, Chayama K
  • Journal Title: Clin Exp Nephrol Volume: 15 Pages: 634-640
  • DOI: 10.1007/s10157-011-0464-7
  • Peer Reviewed
• [Journal Article] IL28B polymorphism may guide pegylated interferon plus ribavirin therapy even after curative treatment for hepatitis C virus-related hepatooellular carcinoma (2011)
  • Author(s): Kawaoka T, Aikata H, Takaki S, Hiramatsu A, Waki K, Hiraga N, Miki D, Tsuge M, Imamura M, Kawakami Y, Takahashi S, Ochi H, Tashiro H, Ohdan H, Chayama K
  • Journal Title: J Viral Hepat Volume: 18 Pages: e550-560
  • DOI: 10.1111/j.1365-2893.2011.01468.x
  • Peer Reviewed
• [Journal Article] Hepatitis C virus infection suppresses the interferon response in the liver of the human hepatocyte chimeric mouse (2011)
  • Author(s): Tsuge M, Fujimoto Y, Hiraga N, Zhang Y, Ohnishi M, Kohno T, Abe H, Miki D, Imamura M, Takahashi S, Ochi H, Hayes CN, Miya F, Tsunoda T, Chayama K
  • Journal Title: PLoS One Volume: 6 Pages: e23856
  • DOI: 10.1371/journal.pone.0023856
  • Peer Reviewed
• [Journal Article] Rapid emergence of telaprevir resistant hepatitis C virus strain from wildtype clone in vivo (2011)
  • Author(s): Hiraga N, Imamura M, Abe H, Hayes CN, Kono T, Onishi M, Tsuge M, Takahashi S, Ochi H, Iwao E, Kamiya N, Yamada I, Tateno C, Yoshizato K, Matsui H, Kanai A, Inaba T, Tanaka S, Chayama K
  • Journal Title: Hepatology Volume: 54 Pages: 781-788
  • DOI: 10.1002/hep.24460
  • Peer Reviewed
• [Journal Article] Impact of viral amino acid substitutions and host interleukin-28b polymorphism on replication and susceptibility to interferon of hepatitis C virus (2011)
  • Author(s): Hiraga N, Abe H, Imamura M, Tsuge M, Takahashi S, Hayes CN, Ochi H, Tateno C, Yoshizato K, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: Hepatology Volume: 54 Pages: 764-771
  • DOI: 10.1002/hep.24453
  • Peer Reviewed
• [Journal Article] Inverse association of IL28B genotype and liver mRNA expression of genes promoting or suppressing antiviral state (2011)
  • Author(s): Abe H, Hayes CN, Ochi H, Tsuge M, Miki D, Hiraga N, Imamura M, Takahashi S, Kubo M, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: J Med Virol Volume: 83 Pages: 1597-1607
  • DOI: 10.1002/jmv.22158
  • Peer Reviewed
• [Journal Article] Variation in the DEPDC5 locus is associated with progression to hepato cellular carcinoma in chronic hepatitis C virus carriers (2011)
  • Author(s): Miki D, Ochi H, Hayes CN, Abe H, Yoshima T, Aikata H, Ikeda K, Kumada H, Toyota J, Morizono T, Tsunoda T, Kubo M, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: Nat Genet Volume: 43 Pages: 797-800
  • DOI: 10.1038/ng.876
  • Peer Reviewed
• [Journal Article] Achievement of Sustained Viral Response after Switching Treatment from Pegylated Interferon alpha-2b to alpha-2a and Ribavirin in Patients with Recurrence of Hepatitis C Virus Genotype 1 Infection after Liver Transplantation : A Case Report (2011)
  • Author(s): Kawaoka T, Hiraga N, Takahashi S, Takaki S, Tsuge M, Nagaoki Y, Hashimoto Y, Katamura Y, Muki D, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Aikata H, Ochi H, Tashiro H, Ohdan H, Chayama K
  • Journal Title: Intervirology (Elpub 25 Aug.) Volume: (Epub)
  • DOI: 10.1159/000328661
  • Peer Reviewed
• [Journal Article] Treatment strategy for early hepatocellular carcinomas : comparison of radiofrequency ablation with or without transcatheter arterial chemoembolization and surgical resection (2011)
  • Author(s): Tashiro H, Aikata H, Waki K, Amano H, Oshita A, Kobayashi T, Tanimoto Y, Kuroda S, Tazawa H, Chayama K, Asahara T, Ohdan H
  • Journal Title: J Surg Oncol Volume: 104 Pages: 3-9
  • DOI: 10.1002/jso.21745
  • Peer Reviewed
• [Journal Article] Antiviral effects of peginterferon alpha-2b and ribavirin following 24-week monotherapy of telaprevir in Japanese hepatitis C patients (2011)
  • Author(s): Ozeki I, Akaike J, Karino Y, Arakawa T, Kuwata Y, Ohmura T, Sato T, Kamiya N, Yamada I, Chayama K, Kumada H, Toyota J
  • Journal Title: J Gastroenterol Volume: 46 Pages: 929-937
  • DOI: 10.1007/s00535-011-0411-0
  • Peer Reviewed
• [Journal Article] Impact of ribavirin dose reduction on the efficacy of pegylated interferon plus ribavirin combination therapy for elderly patients infected with genotype 1b and high viral loads (2011)
  • Author(s): Kohno H, Kouno H, Aimitsu S, Aisaka Y, Kitamoto M, Kawakami H, Chayama K
  • Journal Title: Hepatol Ees Volume: 41 Pages: 626-634
  • DOI: 10.1111/j.1872-034X.2011.00811.x
  • Peer Reviewed
• [Journal Article] IL28B but not ITPA polymorphism is predictive of response to pegylated interferon, ribavirin, and telaprevir triple therapy in patients with genotyrpe 1 hepatitis C (2011)
  • Author(s): Chayama K, Hayes CN, Abe H, Miki D, Ochi H, Karino Y, Toyota J, Nakamura Y, Kamatani N, Sezaki H, Kobayashi M, Akuta N, Suzuki F, Kumada H
  • Journal Title: J Infect Dis Volume: 204 Pages: 84-93
  • DOI: 10.1093/infdis/jir210
  • Peer Reviewed
• [Journal Article] ME3738 enhances the effect of interferon and inhibits hepatitis C virus replication both in vitro and in vivo (2011)
  • Author(s): Abe H, Imamura M, Hiraga N, Tsuge M, Mitsui F, Kawaoka T, Takahashi S, Ochi H, Maekawa T, Hayes CN, Tateno C, Yoshizato K, Murakami S, Yamashita N, Matsuhira T, Asai K, Chayama K
  • Journal Title: J Hepatol Volume: 55 Pages: 11-18
  • DOI: 10.1016/j.jhep.2010.10.017
  • Peer Reviewed
• [Journal Article] Clinical features and prognosis in patients with hepatocellular carcinom a that developed after hepatitis C virus eradication with interferon therapy (2011)
  • Author(s): Nagaoki Y, Aikata H, Miyaki D, Murakami E, Hashimoto Y, Katamura Y, Azakami T, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Takahashi S, Chayama K
  • Journal Title: J Gastroenterol Volume: 46 Pages: 799-808
  • DOI: 10.1007/s00535-011-0384-z
  • Peer Reviewed
• [Journal Article] Significant regression of a cavernous hepatic hemangioma to a sclerosed hemangioma over 12 years :a case study (2011)
  • Author(s): Miyaki D, Aikata H, Waki K, Murakami E, Hashimoto Y, Nagaoki Y, Katamura Y, Kawaoka T, Takaki S, Hiramatsu A, Imamura M, Takahashi S, Ohya T, Sakimoto H, Arihiro K, Chayama K
  • Journal Title: Nihon Shokakibyo Gakkai Zasshi Volume: 108 Pages: 954-961
  • Peer Reviewed
• [Journal Article] Cutaneous complications after transcatheter arterial treatment for hepat ocellular carcinoma via the internal mammary artery : how to avoid this complication (2011)
  • Author(s): Kajiwara K, Kakizawa H, Takeuchi N, Toyota N, Hieda M, Ishikawa M, Tanitame K, Tani G, Suzuki T, Fujikawa K, Aikata H, Chayama K, Awai K
  • Journal Title: Jpn J Radiol Volume: 29 Pages: 307-315
  • DOI: 10.1007/s11604-011-0559-9
  • Peer Reviewed
• [Journal Article] Prediction of response to peginterferon-alfa-2b plus ribavirin therapy in Japanese patients infected with hepatitis C virus genotype 1b (2011)
  • Author(s): Hashimoto Y, Ochi H, Abe H, Hayashida Y, Tsuge M, Mitsui F, Hiraga N, Imamura M, Takahashi S, Nelson Hayes C, Ohishi W, Kubo M, Tsunoda T, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: J Med Virol Volume: 83 Pages: 981-988
  • DOI: 10.1002/jmv.22028
  • Peer Reviewed
• [Journal Article] Common genetic polymorphism of ITPA gene affects ribavirin-induced anemia and effect of peg-interferon plus ribavirin therapy (2011)
  • Author(s): Azakami T, Hayes CN, Sezaki H, Kobayashi M, Akuta N, Suzuki F, Kumada H, Abe H, Miki D, Tsuge M, Imamura M, Kawakami Y, Takahashi S, Ochi H, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: J Med Virol Volume: 83 Pages: 1048-1057
  • DOI: 10.1002/jmv.22069
  • Peer Reviewed
• [Journal Article] IL28 variation affects expression of interferon stimulated genes and peg-interferon and ribavirin therapy (2011)
  • Author(s): Abe H, Hayes CN, Ochi H, Maekawa T, Tsuge M, Miki D, Mitsui F, Hiraga N, Imamura M, Takahashi S, Kubo M, Nakamura Y, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 1094-1101
  • DOI: 10.1016/j.jhep.2010.09.019
  • Peer Reviewed
• [Journal Article] Elimination of hepatitis C virus by short term NS3-4A and NS5B inhibitor combination therapy in human hepatocyte chimeric mice (2011)
  • Author(s): Ohara E, Hiraga N, Imamura M, Iwao E, Kamiya N, Yamada I, Kono T, Onishi M, Hirata D, Mitsui F, Kawaoka T, Tsuge M, Takahashi S, Abe H, Hayes CN, Ochi H, Tateno C, Yoshizato K, Tanaka S, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 872-878
  • DOI: 10.1016/j.jhep.2010.08.033
  • Peer Reviewed
• [Journal Article] IL-28B predicts response to chronic hepatitis C therapy-fine-mapping and replication study in Asian populations (2011)
  • Author(s): Ochi H, Maekawa T, Abe H, Hayashida Y, Nakano R, Imamura M, Hiraga N, Kawakami Y, Aimitsu S, Kao JH, Kubo M, Tsunoda T, Kumada H, Nakamura Y, Hayes CN, Chayama K
  • Journal Title: J Gen Virol Volume: 92 Pages: 1071-1081
  • DOI: 10.1099/vir.0.029124-0
  • Peer Reviewed
• [Journal Article] HCV replication animal model (2011)
  • Author(s): Imamura M, Hiraga N, Chayama K
  • Journal Title: Nihon Rinsho Volume: 69 Suppl 4 Pages: 75-79
  • Peer Reviewed
• [Journal Article] A single nucleotide polymorphism in activated Cdc42 associated tyrosine kinase 1 influences the interferon therapy in hepatitis C patients (2011)
  • Author(s): Fujimoto Y, Ochi H, Maekawa T, Abe H, Hayes CN, Kumada H, Nakamura Y, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 629-639
  • DOI: 10.1016/j.jhep.2010.07.021
  • Peer Reviewed
• [Journal Article] Factors predictive of sustained virological response following 72 weeks of combination therapy for genotype 1b hepatitis C (2011)
  • Author(s): Chayama K, Hayes CN, Yoshioka K, Moriwaki H, Okanoue T, Sakisaka S, Takehara T, Oketani M, Toyota J, Izumi N, Hiasa Y, Matsumoto A, Nomura H, Seike M, Ueno Y, Yotsuyanagi H, Kumada H
  • Journal Title: J Gastroenterol Volume: 46 Pages: 545-555
  • DOI: 10.1007/s00535-010-0358-6
  • Peer Reviewed
• [Presentation] Biological analysis of hepatitis C virus using human hepatocyte chimeric mouse (2012)
  • Author(s): 茶山一彰
  • Organizer: アジア太平洋肝臓病学会議(APASL)
  • Place of Presentation: 台北、台湾(招待講演)
  • Year and Date: 2012-02-19
• [Presentation] Dual Oral Combination Therapy with the NS5A Inhibitor BMS-790052 and the NS3 Protease Inhibitor BMS-650032 Achieved 90% Sustained Virologic Response (SVR12) in HCV Genotype 1b-Infected Null Responders (2011)
  • Author(s): 茶山一彰
  • Organizer: The 62nd Annual Meeting of American Association for the study of Liver Diseases(AASLD)
  • Place of Presentation: アメリカサンフランシスコ(招待講演)
  • Year and Date: 2011-11-07
• [Presentation] Biological analysis of hepatitis viruses using human hepatocyte chimeric mouse (2011)
  • Author(s): 茶山一彰
  • Organizer: 第59回国際歯科研究学会日本部会総会・学術大会(JADR2011)
  • Place of Presentation: 広島(招待講演)
  • Year and Date: 2011-10-09
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/naika1/