2011 Fiscal Year Final Research Report
Evaluation on clarification of transport system of transition metallic ions inside body-defense cells and its association with bio-safety
| Project/Area Number |
21390526
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAGIYA Tadayoshi 岩手医科大学, 歯学部, 助教 (30405774)
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| Co-Investigator(Renkei-kenkyūsha) |
NEZU Akashi 岩手医科大学, 歯学部, 講師 (40264056)
SASAKI Minoru 岩手医科大学, 歯学部, 准教授 (40187133)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 歯学 / 生体材料 / 細胞・組織 / 遷移金属イオン / 遺伝子 / 銅イオンシャペロン / 銅イオントランスポーター / 損傷遺伝子修復酵素 |
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| Research Abstract |
We cultured macrophage in the medium with copper ions, and evaluated gene expression by quantitative PCR. All expressions of cell-membrane transporter SLC31A1 gene, ER transporter ATP7A/7B genes and copper transport chaperon ATOX1 gene declined when the copper content was larger than 200μmol/L. When high amounts of copper ions were not removed(transported) from the macrophage, it was considered that intracellular excessive copper ions might cause DNA damage(DNA-strand breakage), leading to cell death.
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[Journal Article] Proteome analyses of human macrophages exposed to low cytotoxic IC90 copper(2+) ions2011
Author(s)
Taira M., Shimoyama, Y., Kagiya, T., Sasaki, M., Nezu, T., Harada, H. and Kimura, S.
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Journal Title
Dental Mater. J.
Volume: 30
Pages: 293-299
URL
Peer Reviewed
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[Journal Article] Histological and TEM observation of subcutaneous tissues exposed to particulate copper, nickel and titanium2010
Author(s)
Saitoh, S., Sasaki, K., Nezu, T., Taira, M., Shimoyama, Y., Sasaki, M., Kimura, S. and Ishizeki, K.
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Journal Title
J. Oral Tissue Eng.
Volume: 8
Pages: 102-106
URL
Peer Reviewed
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[Journal Article] DNA microarray analyses of the effects of LPS-stimulation and IC50 nickel ions on gene expressions of mouse macrophage-like cell line RAW2642009
Author(s)
Taira, M., Sasaki, M., Sasaki, K., Saitoh, S., Nezu, T., Kimura, S. and Araki, Y.
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Journal Title
Nano-Biomedicine
Volume: 1
Pages: 59-69
URL
Peer Reviewed
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[Journal Article] First-stage genome-wide gene expression analyses of human mesenchymal stem cells exposed to IC50 Ni(2+) ions2009
Author(s)
Taira, M., Kagiya, T., Sasaki, M., Sasaki, K., Saitoh, S., 他4名
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Journal Title
J. Oral Tissue Eng
Volume: 7
Pages: 107-120
URL
Peer Reviewed
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