2011 Fiscal Year Final Research Report
Leneage analysis of postnatal neurogenesis in organotypic culture of hippocampus
| Project/Area Number |
21500299
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tohoku University |
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Principal Investigator |
ISHIZUKA Toru 東北大学, 大学院・生命科学研究科, 講師 (10344714)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 神経新生 / 海馬 / レトロウイルス / スライス培養 / オプトジェネティクス / 細胞系譜 |
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| Research Abstract |
Using the retrovirus vectors encoding EGFP, we labeled proliferating cells in an organotypic slice culture of the postnatal rat hippocampus and tracked an EGFP-labeled single progenitor cell and its descendants as a lineage over a long period. We found that the newly generated neurons became frequently untraceable and this critical traceability period was 2-14 days. It is suggested that the newly generated neurons differentiate into mature dentate granule neurons once they have survived over the critical period. We also created novel genetic probes, which consist of an optogenetic or chemical actuator and an apoptosis detecting reporter. They would help us to reveal the processes that determine the fate of newly generated neurons in an activity-dependent manner.
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Research Products
(10 results)
