2011 Fiscal Year Final Research Report
Local administration of autologous synovium-derived cells improve the structural properties of anterior cruciate ligament autograft reconstruction in sheep
| Project/Area Number |
21500400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KONDO Eiji 北海道大学, 大学院・医学研究科, 講師 (60374724)
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| Co-Investigator(Kenkyū-buntansha) |
YASUDA Kazunori 北海道大学, 大学院・医学研究科, 教授 (20166507)
ONODERA Shin 北海道大学, 大学院・医学研究科, 客員准教授 (00359481)
KITAMURA Nobuto 北海道大学, 大学院・医学研究科, 講師 (80447044)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生体制御 / 治療 / 靱帯 / 腱組織 / 液性因子 |
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| Research Abstract |
Background : It is well known that the structural properties of a tendon autograft deteriorate during the remodeling phase after anterior cruciate ligament (ACL) reconstruction.
Hypothesis : A local application of autologous synovium-derived cells cultured in TGF-betal-supplemented medium may inhibit the deterioration of structural properties of the tendon graft after ACL reconstruction.
Study Design : Controlled laboratory study
Methods : Fifty-two mature sheep were used in this study. In each animal, the right knee underwent ACL reconstruction using the semitendinosus tendon autograft. Then, the animals were divided into 5 groups of 10 sheep each. No additional treatments were applied in Group 1, while fibrin sealant was applied around the graft in Group 2. In Group 3, autologous synovium-derived cells cultured in standard medium were applied around the graft with fibrin sealant, while autologous synovium-derived cells cultured in TGF-betal-supplemented medium were applied with fibrin s
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ealant in Group 4. In Group 5, fibrin sealant containing 20-ng TGF-betal was applied around the graft. Each animal was sacrificed at 12 weeks after the surgery. In each group, 7 and 3 sheep were used for biomechanical and histological evaluations, respectively. The remaining two sheep were used to confirm whether the applied cells infiltrated into the graft at 1 week after surgery.
Results : Confocal microscope observations showed that the applied cells that were labeled before implantation infiltrated into the superficial portion of the graft at 1 week. Biomechanically, the maximum load and the stiffness of Groups 4 and 5 were significantly greater than those of Groups 1, 2, and 3. Histologically, necrotic lesions were observed in the core portion of the midsubstance in Groups 1 and 2. In Groups 3, 4, and 5, we could not find any necrotic lesions in the midsubstance. Conclusions : A local application of autologous synovium-derived cells cultured in TGF-beta1-supplemented medium, or fibrin sealant containing TGF-betal, significantly inhibit the natural deterioration of the structural properties of the tendon graft after ACL reconstruction. Less
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Research Products
(31 results)
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[Presentation] Effects of application of synovial fibroblasts on properties of the graft after ACL reconstruction2009
Author(s)
Kondo E, Hayashi R, Katsura T, Kitamura N, Miyatake S, Arakaki K, Onodera S, Tohyama H, Yasuda K.
Organizer
The 76th Annual Meeting of the American Academy of Orthopaedic Surgeons (AAOS)
Place of Presentation
The Venetian Hotel Resort Casino/Sands Expo Convention Center, Las Vegas, Nevada
Year and Date
20090225-28
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