2011 Fiscal Year Final Research Report
Large DNA molecule introduction into animal, plant and yeast cells using Type IV secretion system
| Project/Area Number |
21510209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | DNA レベルの生物間相互作用 |
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| Research Abstract |
One of Type IV secretion systems (T4SS), which is derived from an IncP type plasmid, was applied as a novel gene introduction method into eukaryotic cells. We found that mitochondrial respiratory mutants in S. cerevisiae showed enhanced transformation efficiency. By using inhibitors of mitochondrial function, we successfully established a simple and efficient gene introduction method into S. cerevisiae from E. coli. In addition, we firstly observed direct gene transfer from E.coli to higher plant cells by applying this T4SS.
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