2011 Fiscal Year Final Research Report
Control of the differentiation pluripotent stem cells(ES・iPS cells) by culture surface coated with functional compounds.
| Project/Area Number |
21560809
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | University of Yamanashi |
|---|
Principal Investigator |
KUROSAWA Hiroshi 山梨大学, 大学院・医学工総合研究部, 教授 (10225295)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | バイオ生産プロセス / 再生医療 |
|---|
| Research Abstract |
In this study we have attempted to control the differentiation of embryoid bodies(EBs) derived from pluripotent stem cells(ES and iPS cells) by functional compounds. The functional compounds hardly influenced the size of EBs. The generation efficiency of beating cardiac cells was low in the EBs formed under 0. 5%-and 10%-PL, and high in those formed under MPC. The expression levels of・-MHC(mesoderm marker) and TTR(endoderm marker) were high in the EBs formed under MPC and low in those formed under PL. However, the activity and pluripotency of the cells at passage stage greatly affected the EB formation. The heterogeneity in cell quality before EB formation made more difficult to properly evaluate the real effect of functional compounds on the EB formation. We reaffirmed the importance of controlling the quality of the cells before use on the experiments.
|
