Analysis of RagA, B/C, D in mTOR signal transduction system

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyushu University
• Principal Investigator: SEKIGUCHI Takeshi 九州大学, 医学研究院, 助教 (60187846)
• Project Period (FY): 2009 – 2011
• Keywords: 分子遺伝

Research Abstract

The objective of this research was to find when, where, and how the G proteins(RagA, B/C, D) regulate mTOR signal transduction pathway. Moreover, we tried to identify roles of interacting proteins of the G proteins in mTOR pathway.
We elucidated significance of Gtrlp and Gtr2p complex formation and interaction to other proteins(Ego3 and Egol) by using G protein mutant proteins.
We found that Gtrlp interacted to Torl complex proteins Kogl and Tco89 in a specific manner. Interaction between Gtrlp and Tco89p was important.
We showed that human RagA, C regulate mTOR and AMPK. It was shown that AMPK regulates mTOR. Here we found that RagA, C could be bridge proteins for mTOR and AMPK.

Research Products

• [Journal Article] 2011 Ubiquitin chains in the DSK2 UBL domain mediate DSK2 stability and protein degradation in Yeast (2011)
  • Author(s): Sekiguchi, T., Sasaki, T., Funakoshi, M., Ishii, T., Saitoh, Y., Kaneko, S., Kobayashi, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 411 Pages: 555-561
• [Journal Article] 2010 New methods for species and sex determination in three sympatric Mustelids, Mustela itatsi
  • Author(s): ekiguchi T, Sasaki H, Kurihara Y, Watanabe S, Moriyama D, Kurose N, Matsuki R, Yamazaki K, Saeki M.
  • Journal Title: Mustela sibirica, and Martes me/ampus Mol. Eco. Res Volume: 10 Pages: 1089-1091
• [Journal Article] 2009 The Gtr 1 p associated with Gtr2p and with Egol p differently
  • Author(s): Wang, YG., Kurihara, Y., Sato, T., Toh, H., Kobayashi, H. and Sekiguchi, T.
  • Journal Title: Gene Volume: 437 Pages: 32-38
• [Journal Article] 2009 Ran GTPase guanine nucleotide exchange factor
  • Author(s): Horiike, Y, Kobayashi, H., Sekiguchi, T.
  • Journal Title: RCC1 is phosphorylated on serine 11 by cdc2 kinase in vitro Mo I. Biol. Rep Volume: 36 Pages: 717-723
• [Presentation] ヘテロ2量体ヘテロ2量体ヘテロ2量体ヘテロ2量体ヘテロ2量体Gタンパク質のGtr1, Gtr2とTor複合体1との相互作用 (2011)
  • Author(s): 関口猛、鎌田芳彰、古野伸明、小林英紀
  • Organizer: 日本遺伝学会第83回大会
  • Year and Date: 2011-09-20
• [Presentation] Torシグナル伝達系のヘテロ2量体Gtr1ーGtr2のGtr1とGtr2の働きの違いの解析 (2010)
  • Author(s): 関口猛、舟越稔、古野伸明、小林英紀,関口猛、舟越稔、古野伸明、小林英紀
  • Organizer: 第33回目本分子生物学会年会
  • Year and Date: 2010-12-10
• [Presentation] Interaction of AMPK gamma to Nop 132, a nucleolar protein (2010)
  • Author(s): Takeshi Sekiguchi
  • Organizer: FASEB summer research meeting
  • Year and Date: 2010-10-04
• [Presentation] ヘテロ2量体Gタンパク質のGtrl, Gtr2は単独で相互作用タンパク質Egol, Ego3に結合する (2010)
  • Author(s): 関口猛、舟越稔、古野伸明、小林英紀
  • Organizer: 日本遺伝学会第82回大会
  • Year and Date: 2010-09-21
• [Presentation] Hideki Kobayashi, Role of Gtrl-Gtr2 complex formation on their biologic function (2010)
  • Author(s): Takeshi Sekiguchi, Yoshiaki Kamada, Yoshinori Ohsumi, Yonggang Wang
  • Organizer: The 24th annual symposium of the protein society
  • Year and Date: 2010-08-03
• [Presentation] Gtrl-Gtr 2複合体のTORングナル経路における機能解析 (2009)
  • Author(s): 関口猛、鎌田芳彰、大隅良典、王永剛、小林英紀
  • Organizer: 第32回日本分子生物学会
  • Year and Date: 2009-12-11
• [Presentation] Gtr 2Gtr1複合体形成にロイシンジッパーモチーフが必須である (2009)
  • Author(s): 関口猛、王永剛、小林英紀
  • Organizer: 日本遺伝学会第81回大会
  • Year and Date: 2009-09-16
• [Presentation] Characterization of Gtr1/2 small G proteins (2009)
  • Author(s): Takeshi Sekiguchi, Yonggang Wang, Naoyuki Hayashi, Hiroyuki Toh, Hideki Kobayashi
  • Organizer: The 23rd annual symposium of The Protein Society
  • Year and Date: 2009-07-27
• [Remarks]
  • URL: http://gtrlgtr2.blog.ocn.ne.jp/blog/