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2013 Fiscal Year Final Research Report

Integrative studies on mechanically mutable connective tissue

Research Project

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Project/Area Number 21570075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionTokyo Institute of Technology

Principal Investigator

MOTOKAWA Tatsuo  東京工業大学, 生命理工学研究科, 教授 (80092352)

Project Period (FY) 2009-04-01 – 2014-03-31
Keywords棘皮動物 / 結合組織 / 力学的性質 / ナマコ
Research Abstract

Mechanically mutable connective tissue (CCT) takes 3 different mechanical states, soft (S), standard (M), and stiff (H). We have isolated from sea cucumbers "NSF", a 2.4 kDa-protein, that caused M to H transition and "softenin", a 20 kDa-protein, that caused M-to-S transition. From these and the images of electron microscopy, we proposed the following molecular mechanism for stiffness changes: the transition from S to M is caused by the aggregation of collagen molecules induced by tensilin, which is antagonized by softenin; the transition from M to H is caused by cross bridging between collagen fibrils by NSF.
The oxygen consumption of 3 states of CCT from 3 different animal classes were measured and compared to that of muscles. The amount to oxygen consumption was lowest in M and highest in S that was far lower than that of relaxed muscles. The posture maintenance through CCT was shown to be the economical way that contributed the low metabolic rate of echinoderms.

  • Research Products

    (21 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (11 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Softenin, a novel protein that softens the connective tissue of sea cucumbers through inhibiting interaction between collagen fibrils2014

    • Author(s)
      Takehana, Y., Yamada, A., Tamori, M. and Motokawa, T
    • Journal Title

      PLoS ONE

      Volume: 9 Pages: e85644

    • DOI

      10.1371/journal.pone.0085644

    • Peer Reviewed
  • [Journal Article] Energy expenditure associated with softening and stiffening of echinoderm connective tissue2012

    • Author(s)
      Motokawa, T., Sato, E. and Umeyama, K
    • Journal Title

      Biol. Bull

      Volume: 222 Pages: 150-157

    • URL

      http://www.biolbull.org

    • Peer Reviewed
  • [Journal Article] Mechanical mutability in connective tissue of starfish body wall2011

    • Author(s)
      Motokawa, T
    • Journal Title

      Biol. Bull

      Volume: 221 Pages: 280-289

    • URL

      http://www.biolbull.org

    • Peer Reviewed
  • [Journal Article] A novel stiffening factor inducing the stiffest state of holothurian catch connective tissue2010

    • Author(s)
      Yamada, A., Tamori, M., Iketani, T., Oiwa K. and Motokawa, T
    • Journal Title

      J. Exp. Biology

      Volume: 213 Pages: 3416-3422

    • DOI

      10.1242/jeb.044149

    • Peer Reviewed
  • [Journal Article] Evidence that water exudes when holothurian connective tissue stiffens2010

    • Author(s)
      Tamori, M., Takemae, C. and Motokawa, T
    • Journal Title

      J. Exp. Biol

      Volume: 213 Pages: 1960-1966

    • DOI

      10.1242/jeb.038505

    • Peer Reviewed
  • [Journal Article] Low oxygen consumption and high body content of catch connective tissue in body contribute to low metabolic rate of sea cucumbers2009

    • Author(s)
      Takemae, N., Nakaya, F., and Motokawa, T
    • Journal Title

      Biol. Bull

      Volume: 216 Pages: 45-54

    • URL

      http://www.biolbull.org

    • Peer Reviewed
  • [Presentation] Connective tissues in echinoderm animals that can reversibly change their stiffness2014

    • Author(s)
      Yamada, A, Takehana,Y, Tamori, M, and Motokawa, T
    • Organizer
      American Biophysical Society
    • Place of Presentation
      San Francisco, U.S.A
    • Year and Date
      2014-02-15
  • [Presentation] A-sea cucumber derived novel protein that softens the cell-disrupted catch connective tissue through inhibiting the interaction between collagen fibrils2014

    • Author(s)
      Takehana,Y, Yamada, A, Tamori, M, and Motokawa, T
    • Organizer
      American Biophysical Society
    • Place of Presentation
      San Francisco, U.S.A
    • Year and Date
      2014-02-15
  • [Presentation] ガンガゼにおけるキャッチ結合組織と棘筋の協調的神経支配2013

    • Author(s)
      渕上芳朗、本川達雄
    • Organizer
      日本棘皮動物研究集会
    • Place of Presentation
      富山大学
    • Year and Date
      2013-12-07
  • [Presentation] シカクナマコの体壁真皮を軟化させるタンパク質の発見2013

    • Author(s)
      竹花康弘、山田章、田守正樹、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2013-09-28
  • [Presentation] ウニにおける神経ペプチド候補物質NGFFFamideとNGIWYamideの効果と局在2013

    • Author(s)
      白木智之、田守正樹、本川達雄
    • Organizer
      日本動物学会関東支部大会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2013-03-16
  • [Presentation] キャッチ結合組織(ナマコ真皮)を軟化させる因子の探求2013

    • Author(s)
      竹花康弘、田守正樹、本川達雄、山田章
    • Organizer
      日本動物学会関東支部大会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2013-03-16
  • [Presentation] Molecular mechanisms of reversible changes in stiffness of echinoderm collagenous"catch"connective tissues2012

    • Author(s)
      Yamada, A, Takehana,Y, Tamori, M, and Motokawa, T
    • Organizer
      日本生物物理学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2012-09-22
  • [Presentation] ウFGFFFamide :ウニのキャッチ結合組織を支配する神経ペプチド2012

    • Author(s)
      白木智之、田守正樹、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      大阪大学
    • Year and Date
      2012-09-13
  • [Presentation] 細胞を破壊したナマコ真皮に対する真皮抽出物の効果2011

    • Author(s)
      竹花康弘、池谷知昭、山田章、田守正樹、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
  • [Presentation] ヒトデ体壁のキャッチ結合組織2011

    • Author(s)
      本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
  • [Presentation] シカクナマコ結合組織の硬化および軟化に伴うエネルギー消費2010

    • Author(s)
      佐藤絵里子、本川達雄
    • Organizer
      日本動物学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-09-25
  • [Book] 生物学的文明論2011

    • Author(s)
      本川達雄
    • Total Pages
      250
    • Publisher
      新潮社
  • [Book] 世界平和はナマコとともに2009

    • Author(s)
      本川達雄
    • Total Pages
      287
    • Publisher
      阪急コミュニケーションズ
  • [Book] ウニ学2009

    • Author(s)
      本川達雄, 他
    • Total Pages
      462
    • Publisher
      東海大学出版会
  • [Remarks]

    • URL

      http://home.t01.itscom.net/motokawa/

URL: 

Published: 2015-07-16  

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